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We narrowed to 919 results for: IRE;

Showing: 561 - 600 of 919 results

  1. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything..., work-life negotiation, and mentorship. We can direct people to the AMA in the future and link to it ...an organizer/promoter was that I didn’t have the direct link to send people until the day of the AMA. This...

  2. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...all the steps and technical considerations are directly applicable to the rat [6]. Technical differences...Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins." Nature...Central PMCID: PMC3082132. 9.Del Bene, Filippo, and Claire Wyart. "Optogenetics: a new enlightenment age for...

  3. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...which is ideal for observing and manipulating the entire developmental cycle. A majority of studies involving...most significant advances is the sequencing of the entire axolotl genome, revealing an abundance of genes...year 2000, becoming the first plant to have its entire genome sequenced (Koornneff and Meinke, 2010). ...

  4. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... the activity of the original, generated using directed evolution (Tei et al., 2023).  PLD can hydrolyze... doesn’t matter. Plus, for folks interested in directed-evolution methods, the activity-based selection... J. C., & Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian cells...

  5. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...really are selling yourself. A sales pitch that requires reading two pages or even a paragraph to get to... Simply stated, what are your aims? You could directly state your official aims, but I suggest distilling... and Structural Biology, and now serves as the Director of Student and Postdoctoral Programs at the Janelia...

  6. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... developed using yeast display technology, are directed to integrins that bind the Arginine-Glycine-Aspartate...2022). By contrast, LVDP-binding integrin α4β1 required and conferred less force, leading cells to spread...integrins, αVβ3 and αVβ5, participated but were not required for cell adhesion. The results surprised the investigators...

  7. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...speaking early and often. Two of my jobs have indirectly resulted from someone seeing me speak in a non-interview... research, “aha” moments, and future research directions. Don’t worry if your work hasn’t been commercialized...does follow the general structure and flow of the entire session. I myself have a terrible habit of saying...

  8. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Brainbow-1.0 in that transient Cre recombination is required to prevent constant inversion of the construct...derivatives of the fluorescent proteins, which are directly trafficked to cell membranes. Membrane trafficking... Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire E, Morin X, Livet J. Neuron. 2014 Feb 5;81(3)...

  9. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...advantage to using direct epigenetic modifiers? As with any experiment, your desired outcome will determine... DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety of ...

  10. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ... in a DNA double strand break (DSB), the event required for frameshift and knock-in mutations. If one ...will generate a ssDNA break which can easily be repaired by cells with a near zero likelihood of mutation...sequencing The only way to quantify off-target effects entirely is to perform whole genome sequencing. While this...

  11. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...guide and the transposon RNA homology both help direct the fusion construct to the new genomic locus. ...indel formation than methods relying on homology-directed repair (HDR), and insertion is scarless, unlike...where those interactions occur in the genome. This requires both targeting a specific genome location and ...

  12. Important Considerations When Using AAVs

    Type
    Blog Post
    ... Is Bitter, but Its Fruit Is Sweet Time If you require a quick onset of expression, AAV may not be the...AAVs for DREADD delivery indicates ~7-21 days are required for the onset of gene expression. However, it ... As single stranded DNA viruses, AAVs normally require second strand synthesis before gene expression ...

  13. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...variable region of an antibody does not recognize the entirety of the target protein: instead, it recognizes ...between the IgG antibody and scFv. Because the entire antibody molecule is not necessary for antigen ...other markers can be injected into an animal, to directly label circulating cells or other cells that are...

  14. Guide to Using Pooled Libraries

    Type
    Guide
    ... and allow you to design additional hypothesis-directed experiments. As you might have guessed from their...For some libraries, plasmid DNA can be delivered directly to the cells of interest. With others, notably...

  15. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...antigen of interest in the given protocol. This requires use of one of the five pillars of antibody validation...intended application — here, flow cytometry — is the desired target, using one or more of the approaches outlined...relative protein expression. Its reliability will be entirely dependent on the selectivity of the antibody used...

  16. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...with two homology arms on each side flanking your desired insertion or mutation. Each arm is around 800 bp... in the HR template. Since the PAM sequence is required for successful targeting and cleavage, changing...

  17. Modular Cloning Guide

    Type
    Guide
    ...assembly step, compatible Level 0 parts are directionally assembled into a Level 1 vector creating a single...highly-tunable GPCR-based biosensors in yeast (requires MoClo-YTK parts for use). Yeast GoldenBraid Cloning...

  19. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein...each and every Addgenie, from our co-ops to our directors, we can't celebrate Addgene without recognizing...

  20. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...John Jumper! The Baker Lab focuses on designing entirely new proteins, improving our understanding of how...John Jumper! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani, G., Stoddard, B. L., & Baker, D...

  21. Summer Fun at Addgene!

    Type
    Blog Post
    ...around the corner. Here at Addgene, we’re always inspired by the new seasons, so we've made some big summer...Landscape for Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite...

  22. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...Collins labs at the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD we've had... - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why Alex decided to work there...

  23. CRISPR Cheat Sheet

    Type
    Blog Post
    ...Components of CRISPR: gRNAs: Sequences of RNA that direct the CRISPR system to cut other DNA or RNA sequences...Nucleases: Proteins that bind to gRNAs and are directed by the gRNAs to cut particular DNA or RNA sequences...

  24. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...below the ordering table. Click on it, select your desired serotype and volume, and submit your request.  ...simply request your plasmid of interest in your desired serotype, place your order when ready, and we’ll...

  25. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ...would be an average of 673 plasmids each day. This requires tremendous coordination amongst the lab team, ...above), but as Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard ...

  26. Editor's Choice, October 2016

    Type
    Blog Post
    ...Increased Postdoc Wages,” Future of Research executive director, Gary McDowell, gives a thorough yet concise rundown...postdocs various academic institutions can afford to hire. It will be difficult for some of these institutions...

  27. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...Matthew J. Sikora (@mjsikora) December 18, 2018 Fireplace from the Dyer Lab If you’re feeling as chilly ..., they turned one of the lab computers into a fireplace scene.  Got our @EmoryChem lab office looking...

  31. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ... an introduction to Addgene with our Executive Director, Joanne Kamens. As a regular contributor of careers...teams at Addgene. An introduction to plasmids with Director of Biology Lianna Swanson was very popular, with...

  32. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...cups of coffee would be consumed throughout the entire 5 day event. Though, to be fair, I think this is... a day, and I had colleagues who were much more wired. Here at #SfN14 there are thousands of posters being...

  34. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...target sites 5' of the 5'NGG3' PAM sequence as directed by a gRNA. 2A-Puro In this plasmid, SpCas9 is ...stand breaks and may better facilitate homology directed repair. You can find all of these variants, as...

  36. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ..., and almost all universities and institutions require them for material transfers. When Addgene entered...and emails it straight to you, or we can send it directly to your institution’s MTA coordinator. Organizations...

  38. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...otherwise ubiquitous promoter. You could make an entirely new mouse line with mCherry controlled by a neuron...gene of interest in neurons. This ability to be paired with any Cre-controlled gene of interest makes ...

  39. Editor's Choice, September 2016

    Type
    Blog Post
    ...smartphone apps you can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember...contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher at ...
Showing: 561 - 600 of 919 results