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We narrowed to 801 results for: MAK

Showing: 561 - 600 of 801 results

  1. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... available as lentiviral plasmids if you want to make a stable cell line. This design allows highly localized...bioRxiv, under a CC-BY-NC-ND 4.0 license.   Just make sure you don’t overdo it! The moderately-enhanced...

  2. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...contributed to this post. Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene...approach requires G418 selection of infected clones, making it unsuitable for most in vivo applications.  Third...

  5. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...easy, but the risk of PCR-introduced mutations can make alternative approaches more favorable if you want...restriction sites. Alternatively, if you want to make your protein non-functional, you may simply introduce...

  6. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ... unmethylated DNA, and more. Many mutations that make these features possible are present in most commercial...commercial strains, especially mutations that make major improvements such as those that increase plasmid...

  7. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...scenarios below for a CRISPR experiment which involves making specific knock-out edits in a gene(s) of interest...off-target frequency of 5%. Your experiment involves making a large sgRNA library to thousands of genes and...

  8. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ... disease states, and targeting the epigenome may make certain processes, like cellular reprogramming of... likely cell type- and context-dependent, it may make sense to try multiple CRISPR tools when setting ...

  9. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...plasmids encoding antibodies from Addgene that you can make on your own.  To narrow your search, first pay attention...knocked down or knocked out. If you are interested in making your own knockout line for validation, please view...

  10. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...exquisite for the so-called base editor Cas9, which makes DNA changes in the absence of dsDNA breaks (Rees... modification of interest, it would be unwise to make conclusions on the basis of the activity of a single...

  13. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...during chemical synthesis of these molecules. To make large amounts of pure product in an affordable way...constitutive and inducible promoters, linkers, and tags makes EcoFlex a great option for those interested in recombinant...

  15. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...immunoglobulin (Ig) heavy chains and two Ig light chains make up each antibody molecule. The Fc region and part...epitopes on the protein. However, each animal will make antibodies against different protein epitopes and...

  16. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...controls needed to pressure-test your FRET assay to make sure it is working? This article addresses these...FRET experiments. However, remember that, if you make an FP fusion, you are inserting about 20-25 kDa ...

  18. Deep Dive: qPCR

    Type
    Blog Post
    ... plateau stage after exponential amplification, making it difficult to properly analyze your data.  To...understanding how the analyses work - you do need to make sure the assumptions in your dataset match the assumptions...

  19. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...The ease and flexibility of the LACE technology makes this system widely accessible for many potential...interest within the INT location. One plasmid to make note of is the INT construct bearing the "Bunch ...previous studies, and provide new insights into the make-up of multiprotein complexes like the EPC1 complex...

  21. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ... While helpful from a biological standpoint, it makes delivering AAVs to the brain difficult. Traditionally... References Asencor, A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & Chaudhari, N. (2022). Selectively...

  24. CRISPR Guide Update

    Type
    Blog Post
    ...section and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...

  30. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...pipette boxes, wire, cable ties) received a festive makeover today✂️❄️🦠thanks to the awesome templates by ...

  37. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...CRISPR X from Michael Bassik's Lab - A new tool for making diverse, localized point mutations. Listen to Our...

  38. CRISPR Cheat Sheet

    Type
    Blog Post
    ...Editors: Modified versions of CRISPR nucleases that make single base changes in the DNA sequence without ...

  39. Our New Antibody Mascot is....

    Type
    Blog Post
    ...suggestions here or by using the hashtag #AntibodyMakesThree on Twitter. As with Blugene and Aavery, we...

  40. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...news (pop culture events of the past year obviously make for good costumes), science and our plasmid collection...
Showing: 561 - 600 of 801 results