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We narrowed to 1,006 results for: RING

Showing: 561 - 600 of 1006 results

  1. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...your experiments. Get organized now! Start by gathering that pile of lab notebooks and binder on your ...frequently. Copy these into an electronic file sharing tool, such as Google Drive or Dropbox, and file...to organize your cryo freezer. No more issues deciphering handwriting or trying to find to where the binder...

  2. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...co-op) is what has brought me, a second year bioengineering student, to Addgene. I am currently working...Addgene, I felt comfortable asking questions without fearing they would be disregarded - everyone was very approachable...comfortable.     Lucie Wilson is a rising junior and bioengineering student at Northwestern University. She is ...

  3. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...disease research and provide hope to patients suffering from these devastating afflictions. Elia Di Schiavi...for peritoneal mesothelioma and thereby relieve suffering from this little-known disease. You can find more...Nabavi and will continue to work to help those suffering from rare diseases. These underrepresented individuals...

  4. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...much easier to get around our site.  Community, sharing, and fun Along with improving search and navigation...the values that make Addgene unique: community, sharing, and fun. The Addgene repository is built on the..., fun, and promote our values of community and sharing.  One big feature upgrade? Blugene now has a full...

  5. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...preferences using HEK293 cells. They started by comparing D10A and H840A total editing efficiency in PAM-in...H840A creates 3’ overhangs in a PAM-out design.  Exploring nickases for homology directed repair The potential... Mary Gearing...

  6. Antibodies 101: Affinity Tags

    Type
    Blog Post
    .... If you decide not to cleave the GST tag after/during purification, be aware that the tag dimerizes in...purifications due to the presence of more naturally occurring histidine-rich proteins in mammals. Mammalian ...possible but requires optimization and often more stringent washing. Nonetheless, this tag can be purified...

  7. Hot Plasmids - October 2022

    Type
    Blog Post
    ...for 2-photon imaging of voltages. This includes monitoring of fast bursts, as well as slow up/down voltage...work also proposes promising avenues for future engineering to shift the spectra and improve the performance...not just because of the title!) The Myc-tag is a string of 10 amino acids, EQKLISEEDL, that can be incorporated...

  8. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ..., had to keep him from running too much as any jarring of his body could initiate very painful FOP flare...donation via the link below. Please continue to help bring HOPE to AJ.   PS: The superhero race is the perfect...running on behalf of AJ Gonzales, a young boy suffering from FOP, raising money for NORD for the past ...

  9. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...obstacles that stand in the way of the doers and by ensuring good communication. A manager must both hire and...delegating tasks and in motivating their doers by ensuring they are as engaged as possible. To learn more...of leader, one needs to take the initiative by bringing and effecting solutions to both known and unidentified...

  10. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...associated genes) system. This popular genome engineering tool offers flexibility, multiplexibility, and...system that scientists have harnessed for genome engineering. The diversity of the CRISPR/Cas systems provides...who has the strongest will to survive. Genome engineering and future directions CRISPR/Cas inspired genome...

  11. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...pR6K ~15-20 R6K* C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300...of replication is referred to as "relaxed" or "stringent" depending on whether the ori is positively regulated...

  13. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ...Agreements, your TTO signs each agreement just once, covering nearly all future requests/deposits. This reduces...Institute  California Institute of Technology Cold Spring Harbor Laboratory  Dana-Farber Cancer Institute...Hutchinson Cancer Research Center Memorial Sloan-Kettering Cancer Center  Monash University  Montana State...

  14. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...different CellTag libraries at multiple timepoints during reprogramming. These library-specific identifiers...different CellTag libraries at three time points during the 28 day study: prior to reprogramming (CellTagMEF...CellTagging. By constructing lineage trees and comparing them with the gene expression changes of more ...

  15. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ...reached out to MIT labs in the Synthetic Biology Engineering Research Consortium. That is how Aditya Kunjapur...robust tools that have practical applications in monitoring the accidental or intentional release of engineered...complementary tools in a time where biological engineering and synthetic biology is expanding rapidly.  ...

  16. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...preventing them from moving forward? Answering these questions will bring you and your audience - via the ...sense of curiosity and wonder why you’re doing and sharing science in the first place?   References and Resources...

  18. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...considerable number of researchers, I had high hopes of securing tenure in a British or Irish University where ...how to sell these products to other scientists. Tailoring my CV for the job To become an attractive candidate...removed my techniques and protocols, courses taken during my PhD and Undergraduate sections and brought in...

  19. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...identify potential career pathways, education and mastering new skill sets must occur by doing work outside...can: Introduce new perspectives in science and engineering Looking at your research from a broader viewpoint...Networking As the number of qualified scientists entering the workforce increases, EAs allow graduate students...

  20. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...common ways to quantify DNA. This method involves measuring the absorbance/transmission of light through a...fluorometer to quantify DNA this way. First, start by pouring your gel containing a DNA intercalating dye (ex... the run time is just a few minutes per sample. During the run, DNA fragments move through a micro- or...

  21. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...from Janelia (jGCaMP7) continue to be useful for monitoring neuronal activity in the form of calcium dynamics...fluorescence and resting fluorescence (achieved through lowering sensor resting fluorescence, while maintaining...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  22. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...serotype for their particular study, which includes comparing the transduction patterns of different AAV serotypes...its calcium-bound state, which was achieved by lowering the basal fluorescence of the sensor. This “contrast...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  23. The MTA at Addgene

    Type
    Blog Post
    ...process. Even though this process is essential to sharing plasmids around the globe, it can be a bit of a...clear expectations from the beginning makes the sharing process smoother, more consistent, and more pleasant...MTA YouTube video for more information. Happy transferring!    Many thanks to our Tech Transfer team members...

  24. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...proteins or cells while having little effect on neighboring cells. The photosensitizer used in this work ...effective killing of dL5** cells without death of neighboring bystander wild-type cells.   Zebrafish experiments...photosensitizers had similar rates of off-target damage to neighboring cells. TL;DR MG-2I-dL5** is a NIR photosensitizer...

  25. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...hypothesized that she could make enzymes controllable by tethering them to Dronpa, a fluorescent protein known to... HMOs are thought to play an important role in nuturing the development of beneficial bacteria in the ...HMO biosensors and displayed her findings at the Spring 2017 ACS meeting. She hopes these biosensors and...

  26. Addgene Depositors Get More Citations

    Type
    Blog Post
    .... While clearly an outlier, this story had us wondering: is there a larger trend here? Do papers associated...Management. She is particularly interested in exploring how innovative tools influence the direction of...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...

  27. Hot Plasmids - January 2023

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    Blog Post
    ...the weaponized T-cells (CAR-T cells) - and then delivering them to a tumor - they used focused ultrasound...staining, and light exposure can negatively affect FPs during these processes. The Liu and Petsko labs recently...only brilliantly bright, but it also withstands denaturing conditions, high temperatures, and other chaotropic...

  28. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on ...aim to alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select...the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs from the Broad...

  29. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... and understand. All of this communication and sharing fits together to make for a transparent and collaborative... for data management plans and for evidence of sharing for grant renewal—but there is clearly room to ... all its different forms as part of graduation, hiring, funding, and promotion processes, I’m confident...

  30. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...pathways can modify the sequence of a gene (thus altering its function). HDR is crucial for certain applications...Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed sgRNA, and...al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery....

  31. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...resulting from the catalysis of a chemical reaction requiring luciferin, ATP and oxygen as substrates. Production... have been identified in various species with differing kinetics, substrate requirements and photon emission...these luciferases have been subjected to further engineering to improve upon the properties of the native ...

  33. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...these omissions, so it is unlikely to be caught during the peer review process, which often focuses more...respond as expected, use of representative images, measuring the main outcomes in several different ways, and... a particular result. Anything else that occurs during the course of the study, say a fifth time has unpredictably...

  34. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...until it is triggered to enter the lytic cycle.   During both of these life cycles bacterial DNA can be ...in a wild type E. coli (Griffiths et al., 2000). During conjugation, genetic material is transferred from...Guillemin. "Multiple phases of competence occur during the Helicobacter pylori growth cycle." FEMS microbiology...

  35. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...ends to insert genes into plasmid backbones, base pairing between these ~4 base overhangs isn’t strong enough... be accessible to allow for complementary base pairing, so SLIC can’t be used if the overhangs would have...ligase! This post was originally written by Mary Gearing in December 2015 and was updated by Emily P. Bentley...

  36. Hot Plasmids - February 2022

    Type
    Blog Post
    ...creates challenges for in vivo delivery and cell engineering applications. CasMINI, a new miniature CRISPR-Cas... Figure 4: Overview of CasMINI for genome engineering applications. Image from Xiaoshu, et. al. 2021...system opens up new possibilities in both cell engineering and gene therapy.  Xiaoshu X, et al. Molecular...

  37. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...do not require breakdown of the nuclear envelope during cell division to enter the nucleus as gamma-retroviruses...AAV also has multiple serotypes (12 naturally occurring and more synthetic capsids are routinely being...lentivirus, retrovirus, adenovirus, and AAV. If you’re wondering more about what applications these viruses are...

  38. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...approaches can be found. As an example, say you are considering either a cellular screen or a selection. A selection...scene, there are other options which are worth considering. RNAi screens are similar to CRISPR, but instead...temperature sensitive screens. These involve raising or lowering the culture temperature to exacerbate a mutant...

  39. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit the number...examined editing efficiency in intact rice plants, comparing traditional gRNA constructs with PTGs. In PTG ... Mary Gearing...

  40. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...of experimental problems or questions you are pondering and be ready to talk about them. People love to... to help especially when solving problems.  During the conference The conference may start in the airport...post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn users notice this sort of...
Showing: 561 - 600 of 1006 results