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Showing: 561 - 600 of 974 results

  1. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...trivia, and everything in between). Eleven years later,  Addgene is a company of fifty instead of three...situation- do we work so hard so we have time to play later? Or, does our play encourage us to work hard? Whatever...employees. The teambuilding culture helps us build relationships and thereby work better together.I realize I...encourage everyone to make an effort to build relationships with their colleagues. It can be easy to get...

  2. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...bi-directionality. For predominately anterograde tracing, biotinylated dextran amines (Veenman et al., 1992) or fluorescent...., 2007; plasmids at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have...overview see Addgene’s AAV guide. AAV particles are relatively small (20 nm) in size which makes them capable...Transport: Cargo-Specific Mechanisms of Motility and Regulation. Neuron 84(2), 292–309. PubMed PMID: 25374356...Veenman, C.L., Reiner, A., and Honig, M.G. (1992). Biotinylated dextran as an anterograde tracer for single-...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    ...Adenoid Cystic Carcinoma, Up-Regulation of pVHL along with Down-Regulation of HIF-1α by NDRG2 Expression...a cell line even decades after it is initially isolated. Regrettably this is not usually the case if you...official biorepositories or have been directly isolated from patients. In these cases, a test known as...

  4. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...Possible roadblocks only become obvious at rather late stages (i.e. postdoc level) and can include the ...non-academic career options require additional study for late career scientists to become more appealing on the.... The issue was with a custom-made reagent to manipulate cells (manufactured by one of the larger biotech...signals are obtained. Or someone may heat an ELISA plate during incubation steps in order to enhance the ...

  5. How to Write a Scientific Review Article

    Type
    Blog Post
    ...it helps you later on to remember where you read particular studies or experiments. Later, you can easily...have to worry about having to hunt down sources later on.  A comment about writing: initially I felt that... all the proteins in the class that showed the relative sizes and the conserved domains. This information...

  6. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... added on a regular basis, so the ecosystem of related tools is always growing. Beyond the ability to ...formatting. In this way data is moved from a static and isolated vehicle and becomes part of the dynamic and connected...understand and/or attempt to reproduce research relating to data deposited into a repository access to ...can be viewed – and conceivably additional work relating to reproducing the original research can be documented...

  7. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...binding specificity: SpCas9 variant Mutations (relative to SpCas9) PAM sequence D1135E variant ...bacterial species Many more Cas9 orthologs have been isolated, and our understanding of their PAMs is shown ...same species. Expanding the CRISPR toolbox The isolation of novel CRISPR proteins has and will continue... an intrinsically high-fidelity genome-editing platform. Genome Biol 19: . https://doi.org/10.1186/s13059...

  8. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...to be translated into Modern Greek to be easily accessible today. Roughly speaking, we translated our software...sprints is themed and the stories in that sprint relate to the theme. For example, we could have a shipping...feature to work. We will then go back to this area later and tidy it up. Conclusion While none of us are...

  9. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...available to many countries, import and export regulations, differing customs processes, and the distances...through the use of popular Chinese social media platforms.  As you can imagine, shipping to so many countries... a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture Collections ... Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial Cultures Restriction Digest ...

  10. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...called CROPseq-multi (Walton et al. 2024). This platform is compatible with enrichment, single-cell sequencing...fast-spiking neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar...donor site; SAS, splice acceptor site; pA, polyadenylation signal. Image reused from Riedmayr et al. 2023...therapies. REVeRT supports advanced gene expression regulation, enhancing both the mechanisms of delivery and...

  11. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...Regardless, the amount of reporter protein activity correlates to both the amount of DNA transfected into the...general, technical replicates can be thought of as "plate controls". They are NOT independent and are typically...transfection of multiple separate wells (within the same plate) with purified plasmid from the same aliquot/preparation... the same source and were incubated on the same plate at the same time. Although the replicate control...

  12. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...requires time and knowledge. In a recent article, we translated our knowledge of electronic lab notebooks into...ELN should allow you to collaborate within the platform and avoid countless emails. Important collaborative...permissions to different lab members to avoid any issues later on. Do you need external teams or partners to access...lab (user, date, time, action) are required for regulatory purposes in many labs. Besides this being of ...

  13. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ... design and engineering of fusion proteins is relatively straightforward. However, generating a fusion...sites or motifs that are necessary for post-translational modifications. Despite these potential issues...fluorescent protein. Thus, scientist began using relatively long linkers between the protein of interest ...C-terminal residues of the fluorescent protein are relatively close together (figure 1). Where should the fluorescent...

  14. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...color, allowing for the fate mapping of cell populations over time. The ability to track single cell dynamics... possible from Brainbow. Tracing clonal cell populations using the Confetti mouse For mammalian systems...not present at the same frequencies. The small population size of CFP++ cells permitted researchers to ... and Lgr5 identify two functionally distinct populations." Proceedings of the National Academy of Sciences...

  15. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...fractionation kit or a lysing protocol developed for isolating proteins from a specific compartment. If your ...blockers, you can run a comparison of different formulations instead of concentrations. Most labs use protein-based... For nitrocellose membranes, you can even give gelatin a shot.  Remember that protein-based blockers degrade...in an assay, meaning that your normalization calculations will not be correct, and you’ll need to adjust... Splice variants Check literature Posttranslational modifications Check literature Highly...

  16. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...harassment Isolation – including preventing access to opportunities, physical or social isolation, withholding... questions in the sections below. Mentoring relationships can help identify your priorities and needs,...regretted it when the same thing happened to her a year later. I hear too many science trainees get warnings and...

  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...certain region or calculate a colocalization factor to assess the spatial relationship between the targets...need more signal, another strategy is to use a biotinylated secondary antibody then label that with fluorescently-conjugated... other antibodies are disrupting the binding of later targets, the sample is being damaged by all the ...

  18. Important Considerations When Using AAVs

    Type
    Blog Post
    .... Speed: AAV transduction experiments are relatively quick. We have found that expression occurs quickly...AAV selection, there are three important factors related to location. Location (1): What tissue? Location...tools for neural tracing in very specific cell populations. Find Ready-to-Use Retrograde AAV Preps at Addgene...and Mark A. Kay. "Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene...

  19. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...sequence but not the amino acid sequence of the translated protein.  These changes can be 5’ or 3’ of the...strains, expression of exo, beta and gam is tightly regulated by the endogenous phage promoter pL and repressor...for a mobile recombineering system, but tight regulation of expression is required for a successful experiment...endogenous red promoter pL and the cI857 repressor to regulate expression. An antibiotic can be used to select...

  20. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... day or a week planning an experiment, even a relatively simple experiment. However, by taking this time...surprising findings when studying a seemingly unrelated problem. However, you have finite time and resources...background slides. If you don't have much new data, speculate on what you hope to accomplish and how your research...satisfying. Finally, many PFs are in committed relationships and/or have started a family. Those people are...

  21. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...structural changes in the ribosome during protein translation (Ermolenko et al., 2007). All that is required... UAG) engineered into a desired site. During translation, the novel tRNA/unnatural amino acid finds the...which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET efficiencies ...applications." Current Chemical Genomics and Translational Medicine 3.1 (2009). PubMed PMID: 20161833. ...

  22. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...hope that these perspectives, the optimism they encapsulate, and the solutions they promote help motivate...infertility or to argue against unnecessary clinic regulations, evidence-based research is vital in combating...Greenbaum Science and evidence-based policy and legislation hit home hard in Michigan, where programs like...worried that, because we were speaking out against legislators and because the march had been so well-publicized...

  23. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...The machine is useful for distinguishing cell populations that have been tagged with different fluorescent...The optimization of GFP and its derivatives by the late Roger Tsien changed everything. For the first time...between 1,000 nm and 1,200 nm, where there is relatively little tissue absorption, weak tissue scattering..."Monitoring of blood vessels and tissues by a population of monocytes with patrolling behavior." Science...

  24. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ...LinkedIn. We are also exploring other social media platforms with active scientific communities and welcome...functionality and features that previously made the platform a mainstay of conversation in the scientific community...considering a gradual move off X as a response to major platform changes, such as limiting access and increased...

  25. The 12 Days of CRISPR

    Type
    Blog Post
    ...celebrate the 12 Days of CRISPR with some of the latest and greatest in CRISPR tools and resources! Ready...gave to me: Enhanced prime editing systems by manipulating cellular determinants of editing outcomes🎵 ...variants for targeted RNA degradation with minimal collateral effects🎵 On the 1st day of CRISPR, Addgene gave...

  26. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...cells. The goal is to use cells that can be easily isolated from donors. Apart from fibroblasts, human keratinocytes...epithelial cells from urine are some of the easily isolated somatic cells that have been reprogrammed to iPSCs...the reprogramming factors) More Chances of accumulation of harmful mutations More, due to selection ...from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci...

  27. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...Abhi Aggarwal gets the reader up-to-date on the latest version of the iGluSnFR glutamate sensorRUBY-Red...CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest blogger Daniel M. Sapozhnikov tells us...modifications!) as a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically...

  28. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...the scientific community. Here are a few virus-related protocol videos that may help to save you time ...AAV preparation. For detailed instructions and calculations on how to prepare a plasmid stock used to generate...with tissue cultures. To keep up on all of the latest protocol videos, lab tips, and more video content...

  29. Deck the Lab 2022!

    Type
    Blog Post
    ...Früh (@Michelderapfel) December 19, 2022   Congratulations to all our #DeckTheLab contestants!           ...tubes, hang up your pipettes, and seal up those plates – it’s time to turn your attention to decorating...decorations and that you’re following all safety regulations. That’s one sticky situation our customer service...

  30. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...t cross the blood-brain barrier. By lowering circulating levels of kynurenine in the body, the brain is...Addgene. References: Skeletal muscle PGC-1α1 modulates kynurenine metabolism and mediates resilience ...doi: 0.1016/j.cmet.2014.09.012. PMID: 25295784 Related Materials: PGC-1a Plasmids      ...

  31. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ... CRISPR, there's a pretty good reason for that. Lately, significant advances in the understanding and ...another very different bit of CRISPR news was circulating. Scientists at North Carolina State, in the labs...as an even more precise and useful tool. A week later, researchers at the Broad Institute and MIT published...

  32. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...incubation step and go straight to plating if your transformed cells are being plated on agar with ampicillin selection...competent cells, don’t forget to do a negative control (plate your cells without adding plasmid) to make sure ...

  33. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...spooky science stories Zombie ants Ophiocordyceps unilateralis is a fungus that infects ants and controls their...to female. Then, it detaches from the gills and latches onto the base of the tongue where it pierces the...References Biron, D. G., et al. "Behavioural manipulation in a grasshopper harbouring hairworm: a proteomics...

  34. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...that really needs it, but then it might to be too late,” Sacher says. As the labs in the directory step...phages or with phages targeting organisms closely related to the clinical sample. For treatment, it is best...phages for clinical use. There are also different regulations from country to country when it comes to administering...

  35. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    .... With this information in hand, you can then formulate a plan to further develop your transferable skills... for career planning, it shouldn’t be used in isolation. Without clear dialogue between trainee and PI...10.1016/j.molcel.2015.04.025. PubMed PMID: 26046646. Related blog posts: Extracurricular Activities for a Strong...

  36. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...on the Central Dogma of Biology, I would stop by later this year when it is displayed!).  The Addgene themed... intention of ending the celebration. The party later moved to State Park, where I had a great (maybe ...helped bring it to fruition. The concept, though relatively simple, changed the course of scientific research...

  37. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...promoter regulating the gene of interest with a constitutive, robust M24 promoter. Originally isolated from.... In total, three MCS regions permit multiple regulatory elements to be inserted, further customizing ...

  38. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ...sequence. In contrast, Dre recombinase, the close relative of Cre, displayed an excision efficiency of only...Multiple new site-specific recombinases for use in manipulating animal genomes. Nern A, Pfeiffer BD, Svoboda...Drosophila cells by estrogen-dependent activity regulation. Heidmann D, Lehner CF. Dev Genes Evol. 2001 ...

  39. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ... be a bad fridge freezing your samples, or a dysregulated water bath boiling your experiments, just about...the LEGOs were mirrored by the nearly infinite regulatory capabilities of gene pathways just waiting to... husband Enzo will spend a few days editing and later looking for songs and sounds to synchronize with...

  40. Hot Plasmids - October 2022

    Type
    Blog Post
    ...-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21 positions...'s more than 50 antibodies target neuroscience-related proteins, but we recently added several broadly...here!   Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-...
Showing: 561 - 600 of 974 results