...and departments will have qPCR machines available for use. ddPCR machines are not as commonly available...measurement of the low-affinity Fc gamma receptor genes FCGR3A and FCGR3B. PloS one, 10(1), e0116791. https...

...generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase chain reaction, qPCR...interest  Molecular Cloning Site-directed mutagenesis Molecular Diagnostics qPCR Quantify starting... PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase...

...system, in which the required adenoviral helper genes are supplied on two different packaging plasmids...pHelper plasmid containing Ad E2A, E4, and VA RNA genes; and the pRepCap plasmid containing replication ...replication factors and serotype-specific capsid genes.  In this helper-free system, HEK293T cells, expressing Ad...

...The degradation tag (dTAG) system functions by harnessing the power of a novel degrader of the 12 kDa protein...round of evolution (AID-2) to reduce some of the leakiness associated with the system, but the general concept...all the tags discussed here and profile their effectiveness with over a dozen different proteins. In the...

...are millimeters in thickness (8,9).  In addition, there is a drop in image brightness with increasing depth... Ultramicroscope.  In addition, adjusting the thickness of the laser sheet and using multi-view imaging...

...students learned something. Based on the initial guidelines I received from the teachers, I formulated the...own wonderful tangents. Some groups asked about vaccines, others were curious about the agricultural repercussions...special thanks to Joanne Marks, Jonathan Marks, and Vanessa Bullard of Chenery Middle School for their tremendous...

...editing from the Cas13b REPAIR system To test the robustness of REPAIRv1, the lab targeted 34 pathogenic G...cytosine deaminase. Three rounds of rational mutagenesis on the ADAR2DD residues that contact the RNA ...RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A mutation in RESCUE...

...teammates want to be assured that you will contribute openness, clarity, approachability, and professionalism...you can learn more about possible strengths and weaknesses, and view everything as a learning experience... weaknesses...

...should be run in duplicate or triplicate. The closeness of the data points generated by replicate values...be linear or nonlinear. BCA assays have linear trendlines while those for ELISAs and other enzymatic assays...advanced graphing software designed to plot complex trendlines. If you don’t have access to this kind of software...

... Joseph Provost, then a new faculty member at Minnesota State (and now Professor of Chemistry and Biochemistry...my students published their work identifying new genes required for stem and progenitor cell maintenance...for increasing diversity and inclusion in STEM disciplines (Bangera & Brownell, 2014; Duboue et al., 2022...

...adapted for a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner...method of tagmentation for next-gen sequencing pipelines (e.g. using Tn5), and as knock-in tools for human...plasmid (pEffector) expresses all seven required genes and the CRISPR RNA under the same promoter, and ...

...studying transcriptional and translational dynamics of genes and proteins in living plants, to complementing ...process right the first time. I’ll lay out some guidelines here. A detailed transformation protocol, as ...potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop....

...hybrid system (also called the interaction trap), genes encoding proteins of interest(POI’s) are fused to...variations, including many that use auxotrophic rescue genes as reporters and some that repress instead of activate... Vectors Read our Blog Post About Tagging Yeast Genes ...

...areas (protected) as opposed to brightly illuminated ones (risky). They also prefer to explore novel environments... (BDNF). BDNF is a small protein involved in neurogenesis, neuronal survival, and synaptic plasticity.... insights into the complex relationship between genes, neurons, and behavior. There are several other ...

...coat! These are the things the rest of the world imagines when they think “scientist.” But leaving the bench...that at a desk, it is much easier to stick to timelines. This is probably because you don't have to worry...transferable skills guide Read blog posts about career readiness Resources at Addgene.org Find careers at Addgene...

...Austrian physician, Andreas Rett, who tried to raise awareness and published his observations in a German medical...global transcriptional regulator of thousands of genes. Studies have suggested roles in transcriptional..., Zoghbi HY (2013) An AT-Hook Domain in MeCP2 Determines the Clinical Course of Rett Syndrome and Related...

...(Chen et al., 2018). Both SHERLOCK and DETECTR harness the promiscuous cleavage and degradation of neighboring...ability to cleave non-specific (trans) ssDNA and harnessed this ability to create a DNA detection platform...Kellner MJ, Tan AL, Paul LM, Parham LA, Garcia KF, Barnes KG, Chak B, Mondini A, Nogueira ML, Isern S, Michael...

...sharing information through the use of popular Chinese social media platforms.  As you can imagine, shipping...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...

...27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome engineering...analysis, they identified 53 potential candidate genes that fell into 3 categories based on the architecture... to bind and cleave target RNAs, although its usefulness in bacterial cells will likely be limited by ...

...options: Early detection, immunotherapy, and angiogenesis inhibitors New research is also going into two...bevacizumab (Avastin). This drug (known as an angiogenesis inhibitor) prevents the growth of new blood ...asbestos. Nonprofits like the Asbestos Disease Awareness Organization (ADAO) are working toward such a ...

...Plasmids can carry one or more antibiotic resistance genes, which confer resistance to a specific antibiotic...

... comb, run the gel on the slower side, and skip lanes between samples. In addition to a DNA ladder standard...

...signal amplification. Another reason is cost effectiveness. Generating and validating primary antibodies...outcome of your assay. So how do you choose good ones? Choosing a secondary is often much more straightforward...non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody will ...

... post. Figure 2: iGluSnFR3 displays enhanced brightness, expression, and sensitivity compared with earlier...2P has faster response kinetics and increased brightness, sensitivity, and photostability under two-photon...screen, selected for increased voltage response, brightness, and photostability. It enables sustained deep-tissue...

...my days as an undergrad working with cancer cell lines. As I started planning out my own experiments in...when prioritizing, you should always be aware of deadlines and think about what they mean in terms of the...favorite podcasts. JUST GET STARTED - One Harvard Business Review article on procrastination recommends testing...

...are amenable to genetic manipulation, nor can all genes be easily knocked down or out.   Example: You just...data that convinced you of your antibody’s trustworthiness. You may also consider sharing your validation..., Anderson AP, Cordell JL, Cragg MS, Šerbec VČ, Jones M, Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual...

... possible in a wide range of organisms and cell lines. In C. elegans, one of the model organisms which...several aspects of editing. First, they devised guidelines for designing single-stranded repair templates...on gene regulation in nematode molting and spermatogenesis. His group also builds tools to improve genome...

...transition Base changes between purines (A ↔ G) or between pyrimidines (C ↔ T).   Base transversion  ...conducted a CRISPRi screen to identify endogenous genes that impact CGBE efficiency. In addition to using...

...Corning 25-015-CI) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

...Created by Joanne Jary from Lab Support at the John Innes Centre, and submitted by the Lab Support Team at...at the Centre.     Lab support team @JohnInnesCentre rule pic.twitter.com/WMb97fpUOI— Sam Mugford (@samtmugford...

...research (check out this article in the GSA’s blog Genes to Genomes about a TMPRSS2 plasmid from Roger Reeve...blog posts showcasing the work of depositors) Wednesday: Why deposit? Thursday: Throwback Thursday (Blue...

...AAV9 is so strong that you can even see dendritic spines!  Curious? Watch our AAV Data Hub Challenge recording..., when published, will meet FAIR data-sharing guidelines. But what if my AAVs didn't work?  We love negative...

... 3,000 antibodies and associated hybridoma cell lines. The DSHB was created by the National Institute ...clonal cells are screened to identify hybridoma lines, each producing a monoclonal antibody against a ...

...simultaneous activation and/or repression of multiple genes, and more. The researchers now plan to adapt the...powerful tools to modify the expression of multiple genes at the same time.” For more details on how to apply...

...rules for naming plasmids but here are some good guidelines to follow in order to ensure that people can ...of published and commercially available empty backbones, visit Vector Database. Step 2: Insert name  Include...

...library (~60,000 gRNAs) that targets ~4,000 E. coli genes with ~15 gRNAs per gene and includes ~10,000 gRNAs...APOBEC1 causes genome-wide deamination of RNA cytosines in human cells. Thus, they engineered two CBE ...

...online from your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at...to generate the legal agreement (MTA), which streamlines the process and saves you time. Anyone affiliated...

...biggest changes, there are plenty of less obvious ones to discover as you browse. You may find one of your...choices informed by Web Content Accessibility Guidelines (WCAG).  But don’t take our word for it — check...

...the DNA sequencing reaction should be repeated. Guidelines to help with DNA sequencing troubleshooting and...Most chromatogram viewing programs (even the free ones) allow you to edit the sequence.   I hope these...

...reaction. STAgR is highly customizable as vector backbones, and gRNA structure, numbers, and promoters can...marker excision method for marking distinct cell clones developed by the Michalis Averof lab. It’s an alternative...