CRISPR Guide
Type
Guide
...genomics using CRISPR-Cas9. 2015. Shalem O, Sanjana NE, Zhang F. Nat Rev Genetics . 16(5):299-311. PMID:...Screening in Human Cells. 2014. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl... and a user-defined ∼20 nucleotide spacer that defines the genomic target to be modified. Thus, one can...CRISPR was originally employed to knock out target genes in various cell types and organisms, but modifications...extended CRISPR to selectively activate/repress target genes, purify specific regions of DNA, image DNA in live... Cas protein you use. We'll use the popular S. pyogenes Cas9 (SpCas9) as an example, but check out our...insertions or deletions (indels) at the DSB site. The randomness of NHEJ-mediated DSB repair has important practical...