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  1. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview... because sometimes companies have relaxed dress codes for interviews. You can also ask HR for clarification...

  3. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...reporter gene, which replaces the targeted gene and provides a way to track successfully engineered cells. ...genotype each animal to identify the ones with the desired genotype. Inducible and conditional systems Conditional...

  4. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...perform differently over time), compatible with the desired applications (such as immunohistochemistry (IHC...antibodies, we have developed a criterion that includes accuracy, strength, and reproducibility. We test...

  6. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...virus will not only ensure that your infection is designed in the best possible way, but it may also save...the transgene itself. Many researchers prefer to design primers that target the common features of viral...

  8. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ... of the DNA with a high frequency of C and G nucleotides). The conversion of cytosine to 5-methylcytosine..., which binds selectively to lysine-methylated peptides. This construct is in the middle of a FRET pair...

  9. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...success You’ve put a great deal of thought into designing your poster – now it’s time to practice your delivery...stepping into a sea of posters – where do you begin? Design a game plan Prior to attending the conference, ...

  10. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...plasmid recombination, but are also endA+. endA encodes a thermostable periplasmic endonuclease that can...exchange will prevent your cultures from reaching the desired density. The volume of your flask or culture tube...

  11. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...After this initial application, Stelzer’s group described the single-plane or selective-plane illumination...and acquire images of a sample from two opposing sides and merge the images, such as in the Ultramicroscope...

  13. AAV for Neuronal Tracing

    Type
    Collection
    ...Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...

  14. DNA Purification Without a Kit

    Type
    Blog Post
    ...plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly identical to that... Image: Kim and Morrison, 2009. This method provides DNA yield and quality similar to that obtained...

  15. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...region In 2013, the Hodaka Fujii Laboratory first described a method to purify a specific genomic region using...plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific genomic...

  16. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...case, a buffer exchange can be performed using a desalting column or an ultrafiltration concentrator. As ...suggests, you may also concentrate your prep to the desired concentration using these columns. If you are conjugating...

  17. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Biology (gDNA → mRNA → protein): their roadmap includes RNA → DNA (Cooper & Temin, 1974), as one of its...is a turn of phrase — “pseudotransduction” — to describe the direct protein transfer of RT, which would...

  18. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...improve antibody binding for some targets, they can destroy it for others. That said, organic solvents’ impact...critical to the outcome of your IF experiment and deserve careful thought and attention.   * There are other...

  19. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...LLC, a grant professional development firm that includes the social media community GrantChat. She has ... from administration and fundraising to program design and evaluation. She and her team at J. Miller &...

  22. Antibody Production

    Type
    Collection
    ...protocols, titering methods, and quality control are described below. Information for a specific antibody can...

  23. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...map these neuronal networks. For almost three decades, recombinant Adeno-associated virus (AAV) has been...symbol of opposition to the teaching of intelligent design in public schools and was started in 2005 in Kansas...

  24. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ..., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also...without being too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting ...

  25. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...tumors.  This process is not quick. It can take decades from the first exposure to asbestos to the time...T-cells to more easily recognize and cause the destruction of cancer cells. Pembrolizumab has shown some...

  26. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...around $45,000, but as I’ve discussed elsewhere (slides here) there are many postdocs paid at much lower...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...

  27. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...updated Aug 20, 2020. Cas9 can be used to modify any desired genomic target provided that (1) the sequence is... may be unintentionally mutated along with the desired target locus. The PAM sequence is of particular...

  28. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides a method to detect nucleic acids with high sensitivity...per second. A quantifiable fluorescent signal designates the presence of your DNA of interest, in this...

  29. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ... Environmental Plasmids blog). Scientists have designed small, high copy incompatible plasmids that lead...Pubmed PMID: 25889267 Ebersbach G, Sherratt DJ, Gerdes K (2005) Partition-associated incompatibility caused...

  33. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...staining. Antigen-containing samples often include undesirable molecules that may bind non-specifically to assay...specific than other methods. In addition, the assay includes both primary and secondary incubation steps, increasing...

  34. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...replication. For example, the plasmid ColIb-P9 encodes the protein RepZ to drive its own replication. ...

  35. Viral Production at Addgene

    Type
    Blog Post
    ...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...) are generated from lentiviral preparations (described above) that are subject to a concentration step...

  36. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...best to start from scratch. Obtain a vial of the desired cell line from a reputable distributor such as ...extreme changes in osmotic pressure and pH can destabilize the viral envelope. One drawback of the multiple...

  38. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...flattening the curve, it has become evident that widespread testing is crucial in controlling this pandemic...one temperature. Two Cas12a-crRNA complexes are designed, each binding to one strand of the reverse-transcribed...

  39. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...antibody-based technique, validation and proper experimental design are essential. In this article, we’ll go through...steps include: counterstaining, mounting sample on slides, and confocal   Figure 2: High-level overview...

  40. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... A) Schematic of workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single ...cryo-EM samples from damage at the AWI. Plus, tardigrades!  Find RvLEAMshort here!    Abe, K. M., & Lim...

  41. AAV Packaged on Request

    Type
    Collection
    ...all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification, and high-...

  42. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ... – including undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name...impossible deadlines, unnecessary disruptions Destabilization – including failure to acknowledge good work...

  43. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...single enzyme if your insert is flanked on both sides by that enzyme’s restriction sites, but the insert...the orientation of your insert. You may want to design a diagnostic digest for this purpose. Ideally, ...

  44. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial Expresses Aquamarine...

  45. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... are more involved in the science, experimental design, and execution. Can lead to an independent project...advice Give yourself permission to pursue your own desired path. Careers in science are also not limited to...

  47. Viral Production

    Type
    Collection
    ...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...

  48. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... recognition are a few methods by which tumors sidestep immune surveillance: Suppressing Function Antigen...target is never the answer to the multi-level barricades put up by cancer. As mentioned in this article...

  49. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...any neuroscience project or topic of interest. Despite its power and versatility, the technique is remarkably... the optogenetic constructs Expression of your desired optogenetic construct in the brain region and cell...
Showing: 701 - 750 of 983 results