...HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target virtually...either an unprocessed or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown...dementia-associated tau, delivering dCasRx and a 3-spacer array targeting the MAPT exon 10 splice acceptor...

...substrate, they synthesized diacetyl coelenterazine-h. Unlike coelenterazine-h, diacetyl coelenterazine-h does... to the media. Cellular esterase then converts diacetyl coelenterazine-h to coelenterazine-h, producing...

...cell!)—ATP. There are biosensors for intracellular, extracellular, and even the ADP:ATP ratio. Most of... from dopamine to glutamate, all the way to acetylcholine. These sensors have been tested in vivo, in ...

...genetic backgrounds. The standard RNAi vector, L4440, places a target gene between two inverted T7 promoters...’s new plasmid, T444T, contains T7 terminators adjacent to the T7 promoters to prevent the incorporation...it is essential to contain outbreaks when they surface. But detection of the virus costs time and money...

...One of the most pivotal decisions many PhDs face when transitioning from academia to industry is whether...requires deliberate preparation and a willingness to embrace new responsibilities. After my second postdoc,...permission to explore, reflect on your priorities, and embrace these transitions as an integral part of your broader...

...guided by a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases...now, it’s time to cast aside those concerns and embrace a new resource to solve the problem: CasPEDIA, ...

...forms tRNA-gRNA and tRNA-gRNA-tRNA, with short 5’ spacer sequences preceding gRNAs, to test for gRNA cleavage...predicted with no addition of nucleotides to the 5’ spacers. The 3’ ends were modified slightly with either...once, and each <200 bp tRNA-gRNA takes up half the space of a standard pol III-gRNA, which will facilitate...

... There is essentially no better place for a scientist to make new relationships than at scientific conferences...rut of hanging out with only your lab mates (let’s face it, you see enough of them).  Before the conference...students and postdocs. There are many inexpensive/free places to order cards. There are a few good reasons to...

...biggest difficulties that plant biotechnologists face is public perception of genetic modification (GM...and we’d love to hear about the obstacles you’ve faced as a plant scientist, and how you were able to overcome...Bioengineering Follow RevBio on Twitter Like RevBio on Facebook Read more Addgene blog posts about synthetic...

...27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells. The results were even ...into M phase oocytes, thus allowing repair to take place before DNA replication. Indeed, they observed that...articles and commentaries) pop up on Twitter and Facebook, shared by biologists and non-biologists alike...

...quicker to pick up new languages!) Jargon has a place in science communication, but the more you use, ...of detail Again, this is an obvious one on the surface, but it’s quite easy to add in “necessary” detail...communicate. It’s okay (and encouraged) to leave blank spaces or to gloss over some aspects when needed. The ...

...transition to stationary phase, Segall-Shapiro et al. placed a trans-acting RepA protein under the control of...needed sequentially. When Segall-Shapiro et al. placed the genes under the control of a constitutive promoter...production titers decreased. But when the genes were placed under the control of a stabilized promoter, deoxychromoviridans...

...talked with Voigt about this collision that’s taking place between CRISPR technology and synthetic biology,.... The Apollo 11 mission circuitry that took a spaceship to the moon was built on NOR gates. It’s a fundamental... Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 ...

...the complex, such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant...version of which will feature a simplified user interface, expanded numbers of curated molecules, including...over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic assay remains widely...

...and recurrent infections along with a mild or exacerbated inflammatory response which leads to nearly 25%...inhibits a potassium channel, causing release of acetylcholine. Given its unique molecular biology and previous...pathophysiological role of glycans. Follow her on Facebook: @glycoimmunologygroup.     Michael McMurray is...

...ago. I just recently started working in the pharmaceuticals industry after earning my Master’s in 2016 ... like cliff notes, so I use these as a starting place for drafting my stories. In total, I prepare 5-6...arrangements with those you live with to have a quiet place to take your video call.  Expect technical difficulties...

...FRET pair. If you need to free up crowded optical space for multiplexing, GECO sensors are available in ... violet light and (2) exposure to increased intracellular calcium. It has been applied to mark active ...Whether you’re studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry...

...experimenter picks up a mouse from the home cage and places it into the experimental setup, the mouse becomes...Both AAV and lentiviral vectors are used for intracerebral injections. AAV particles are smaller and may...liquor of the cerebral ventricles (ICV, intracerebroventricular) to minimize tissue damage; however, the...

...for you in one place! First, a quick refresher: prime editing is a “search and replace” genome editing..., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand breaks or ...

...module entirely, but instead will have to replace it with a spacer.) Each component available on the Fragmid...and selection markers. Need fewer components? A spacer can be inserted instead, allowing everything to...