We narrowed to 492 results for: ARA-2

...Joachim ended up at the University of Amsterdam Chapter 2 - Joachim’s Academic and career goals. Career advice...microscopy (University of Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes...

...short-read sequencing are highlighted in Figure 2. Figure 2: Overview of short-read sequencing technology...synthesized (Figure 1). Sanger sequencing requires four separate reactions, where a fraction of one of the four...The resulting DNA fragments are pooled and then separated by size using electrophoresis, typically capillary...deconvoluted to identify the bases (and, if necessary, separate different samples), the resulting data, or reads...nucleotide that passed through based on these characteristic drops. Figure 3: Overview of Nanopore...To address this issue, the QC team developed a separate script for manually inputting known innocuous ...

...green squares, purple circles and blue triangles. 2) An antibody immobilized on a bead binds specifically...protein G, a novel IgG-binding reagent. J Immunol 133(2):969-974. Hjelm H, Hjelm K, Sjöquist J (1972) Protein...controls for an IP. When possible, perform the IP in parallel with both a sample known to express the protein...identify any non-specific interactions, perform parallel IPs with beads only and an isotype control antibody...

...diffusion test or Kirby-Bauer susceptibility test (Figure 2), and requires at least 18-24 hours to obtain results...." London: Wellcome Trust & HM Government (2016). 2. CDC Information Pages on Antibiotics 3. WHO Antimicrobial...antibiotic’ can also be used to describe anti-parasitic drugs and anti-fungal drugs, because ‘antibiotic...Resistance can be intrinsic, meaning a basic characteristic that naturally occurs in the bacterium, and...

...from animals or hybridomas.   Figure 2: (Left) Figure 2: (Left) The light chain and heavy chain genes..., Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P...

...due to the presence of additional helper sequences (2-3). As alluded to above, termination and polyadenylation...and translation processes as well.   Figure 2: The conserved eukaryotic polyadenylation signal directs...transcriptional read-through. Chris Voigt’s lab has characterized a set of prokaryotic terminators and deposited...

...from another within a fully stained sample. Figure 2 is an example of the emission spectra for two fluorophores...another during spectral unmixing.     Figure 2: The full emission spectra for BV421 and BV711 on ...experiment, it is important to consider how many parameters you want to use in a given experiment. For very...

... follow the same general cyclical process (Figure 2). The differences typically come in when generating...starting point for the next cycle. Figure 2: General process of antibody directed evolution. Created...module_attribute "schema_version" is_json="true" %}{% raw %}2{% endraw %}{% end_module_attribute %}{% module_attribute...antibodies developed by IPI are extensively characterized and validated, with the application data from...

...the chromosome, ending replication.   Figure 2: Prokaryotic chromosomal replication. Adapted from...Microbiology and Molecular Biology Reviews: MMBR, 62(2), 434–464. https://doi.org/10.1128/MMBR.62.2.434-464.1998...cooperatively to separate nearby AT-rich regions of DNA. In a test tube, the DNA helix can be separated by DnaA...ATP-bound DnaA, the proteins cooperatively can separate the DNA strands. Created with BioRender.com. ...

...gene activation, with some genes experiencing around 2-fold activation levels. Although other methods have.... This method has been shown to activate genes at 2- to 3-fold higher than SAM, SunTag, and VPR (Zhou ...once), however, SAM exhibits activation levels comparable to other popular activation methods (VPR and ...

... digest and compare it to some reliable controls. 2. Viral stocks may be sensitive to freeze thaw cycles...PMID: 24003179. PubMed Central PMCID: PMC3760253. 2. Rohwer, Forest. "Global phage diversity." Cell 113.2...10634178. 8. Murnane, John P., Michael J. Yezzi, and Barbara R. Young. "Recombination events during integration...

...mean you have one copy of the gene; an RQ value of 2 means you have two copies, and so on and so forth....quantitative PCR. Expert Rev Mol Diagn. 2005 Mar;5(2):209-19. doi: 10.1586/14737159.5.2.209. PMID: 15833050...falciparum multidrug resistance-1 gene (pfmdr1). J Parasitol. 2011 Oct;97(5):939-42. doi: 10.1645/GE-2792.1...

...module_attribute "schema_version" is_json="true" %}{% raw %}2{% endraw %}{% end_module_attribute %}{% module_attribute...stereotaxically, STX) to the brain. Figure 2: Selection, prep, and testing of AAV enhancers in ...B., Ben-Simon, Y., Opitz-Araya, X., Martinez, R. A., Way, S. W., Thyagarajan, B., Otto, S., … Ting, J....Resources References Ben-Simon, Y., Hooper, M., Narayan, S., Daigle, T. L., Dwivedi, D., Way, S. W., Oster... J. K., Taormina, M. J., Martinez, R. A., Opitz-Araya, X., Roth, J. R., Alexander, J. R., Allen, S., Amster...E. E., Mahoney, J. T., Kojima, Y., Ding, Y., Somasundaram, S., Miller, J. A., Kalmbach, B. E., Radaelli...

...pHelper: 1 RepCap OR 3 Transfer Plasmid : 5 pHelper: 2 RepCap Select what type of tissue culture dish you...goes, “failing to plan is planning to fail.” Preparation is a key step to any experiment and can help ...

...Addgene swag. Good luck and happy naming!   Fig. 2: The mascot trio!     ...our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent distribution...

...Halloween is only 2 days away and here at Addgene we couldn’t be more excited! As a small, tight-knit...(old board games and movies can provide great character costumes). All of this costume planning is for...

...expressed as a fusion with your favorite protein and (2) a synthetic fluorophore-contianing “ligand” that ...cell 58.4 (2015): 644-659. PubMed PMID: 26000849. 2. Grimm, Jonathan B., et al. "A general method to improve...: 26575238. 5. Crivat, Georgeta, and Justin W. Taraska. "Imaging proteins inside cells with fluorescent...

...connectivity to other data sources used in research; (2) connectivity to other tools used in research; and...by making it easy to get data out of the ELN; and (2) facilitate inter-operability with other research ... delve deeper and look specifically at the characteristics and capabilities an ELN needs to facilitate...

...blotting  In a western, proteins are: (1) separated by size, (2) transferred to a membrane, and  (3) detected...system-specific sandwiches.   Figure 2: The western blot dry apparatus includes a stack of filter paper, ...range to be separated on a single gel.  Immobilizing proteins on a membrane Once separated, the proteins... process. The sample, a mixture of protein is separated in a gel, transferred to a membrane, stained with...tertiary structures remain intact and the protein is separated through a matrix by charge. In a denaturing western...protein is denatured to its primary structure and separated by size with smaller molecules moving more quickly... we will be focusing on denaturing westerns.  Separating a protein mix by size Sodium dodecyl sulfate-...

...to a specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID ...cells will be ready for the spotlight.    Figure 2: Opto-RhoGEFs to control Rho GTPase activity. A) Schematic...cytoskeleton. They provide cells with physical structure, separate chromosomes during mitosis, support transport ... called a katana. Opto-katanin consists of two separate blue light-sensitive systems, VVDfast to control...your protein of interest and express the other separately, then use blue light to generate CRY2-CIB1 oligomers...