Skip to main content
Addgene
Showing: 281 - 300 of 576 results

  1. Bioinformatics at Addgene

    Type
    Blog Post
    ... to keep up with the latest tools. In Addgene’s early days, we would ‘spot-check’ critical regions of ...us to keep pace with our current need to analyze nearly 300 sequences each week. Our internal software ...

  2. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...easier to find materials and learn about them. As early as 2007, we began grouping similar tools together...12 guides available on our website, which are regularly used by scientists around the world. Collectively...

  3. Hot Plasmids Spring 2024

    Type
    Blog Post
    ... Janelia, are now available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into...

  4. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...the introduction of this mutation can be any site early in the coding region of the gene of interest. This...Repressing target genes using dCas9-based repressors Early experiments using dCas9 in bacteria demonstrated...

  5. The Challenges of Cell Culture

    Type
    Blog Post
    ... is still a problem today. It is now known that nearly 500 cell lines suffer from contamination with HeLa...out on low passage cells and this means you can regularly discard the cells you are working with because...

  6. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...American Type Culture Collection and freeze as many early passage vials as possible. One problem that often...can make this assumption incorrect. One of the earliest steps in lentiviral transduction is the interaction...

  8. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...between cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...

  9. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...the use of bright red fluorescent proteins (e.g. mScarlet) or infrared fluorescent proteins (Chernov et ...interfere with the function of the protein. This is clearly the case when proteins undergo post-translational...

  10. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...vehicles for episomal gene expression and are particularly useful for gene delivery to the nervous system...is composed of single stranded DNA, which is particularly well suited for strand invasion, a key step ...

  11. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...how much time you can possibly handle doing a particularly annoying task and then dedicating that much ...dinner plans at 6 pm, you had better get started early enough to finish everything you need to do by 6 ...

  13. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...conducted a large scale sequencing and concluded that nearly all disease-linked human genes have counterparts...the most popular model amphibians in science with early research providing insight into some of the most...

  14. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...distancing. With countries like South Korea, one of the earlier epicenters of the outbreak ultimately flattening... is a one-pot, one-temperature reaction, and is nearly single-molecule sensitive. In the case of HIV-1...

  16. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...other videos. For starters, I hadn’t anticipated nearly every webcam recording only 1280x720 resolution... to labs that need them. The lab re-opened very early in the pandemic with some incredibly strict safety...

  18. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...while requiring a lower concentration of sample — nearly ten times less protein. While the addition of LEAs...Methyltransferase (CHARM), with several advantages over the earlier system.  Rather than overexpressing an exogenous...

  19. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... glycosylation patterns. Surface labeling is particularly useful for classifying and sorting cells by ...Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects ...

  20. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... under CC-BY 4.0 license.   Building off their earlier work on Rab11, the team used optogenetic clustering...constructs to the plasma membrane or lysosomes (the earlier article also provided CIB constructs for targeting...
Showing: 281 - 300 of 576 results