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We narrowed to 802 results for: Clu

Showing: 281 - 300 of 802 results

  1. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...viral vectors in the lab. Advantages of lentivirus include a large genetic capacity and the ability to transduce...CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: lentiCRISPR... observed in small trials for other diseases, including the common hematopoietic disorders sickle cell...

  2. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ... and management process or the whole process, including grant readiness, funding opportunity research,...grant training, mentoring and coaching services, including Smartegrants, to help those entering the Grants...LLC, a grant professional development firm that includes the social media community GrantChat. She has ...

  3. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...scientists employ GFP for a wide number of functions, including: tagging genes for elucidating their expression...detect a variety of intracellular conditions, including ion (such as Ca2+) concentrations and pH, using...selection: Expression constructs like plasmids often include GFP as a marker to help identify which cells have...

  4. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ...(GTA). The GTA has a wealth of information and includes annotated images of the brain sections describing...enhancers and their neuroscience research. This includes other data, tools, and resources. Luckily for ...are also more resources available at Addgene, including the Allen Institute for Cell Science Plasmid Collection...

  5. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...N-glycosylase (UNG) enzymes. Various other proteins included in different CGBE tools allow the researcher to...again, this post has a lot of acronyms, so we've included a glossary at the end of this post! Figure...for their application from among many options.  Conclusion With these advances, every base can now be directly...

  6. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...many types of pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA libraries...viruses are sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests specifically...aliquot to full use! Common conditions to optimize include incubation time, antibiotic conditions, and amount...

  7. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...previously not associated with ricin regulation, including almost all genes involved in the production of...non-targeting guides and 6,750 safe-targeting guides were included in the library. Safe-targeting guides were designed...activity, Morgens et al drew some generalizable conclusions about off-target activity, demonstrate safe-targeting...

  8. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...rather late stages (i.e. postdoc level) and can include the pressure to publish in high-impact journals...for insights from my time in technical support including tips, advantages, and disadvantages of this overall...without being too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting ...

  9. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...proteins. Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance ...can be used for an array of detectable proteins including fluorescent proteins, antibiotic resistance, and...

  10. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...replication control that are not necessarily mutually exclusive. In the first, the replication initiation factor...Asymmetric plasmid loss occurs for several reasons, including one plasmid blocking replication of another and...incompatibility caused by random assortment of pure plasmid clusters. Molecular Microbiology 56:1430–1440 . https:/...

  11. Bioinformatics at Addgene

    Type
    Blog Post
    ... bioinformatics pipelining In many use-cases, including ours at Addgene, the overall purpose of a data...workflow management system for scientific pipelines, including bioinformatics. We loved the parallels between...language.  The modern architecture of Nextflow included built-in support for containers, such as Docker...

  12. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...If you choose this strategy, it’s important to include the proper protein expression elements (ribosome...entry clones available for many popular genes, including Hs.KRAS4a. Use our website to search for your ...Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression Systems...

  13. The Challenges of Cell Culture

    Type
    Blog Post
    ...the origin and propagation of the line, and can include assays for authentication and contamination) and...International Cell Line Authentication Committee includes a database listing all cross contaminated or misidentified...working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune, and Infors & Applikon...

  14. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...expression strains to accommodate high protein levels including:  ompT: Strains harboring this mutation are deficient...Rhamnose-tunable T7 RNAP expression alleviates inclusion body formation. Expression vector cannot have ...insoluble globs of misfolded protein are known as inclusion bodies, and can be recovered and purified using...

  15. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...The first time I heard about FRET during a journal club, my guitarist brain automatically thought about ...and several other pairs have been used since - including: mCerulean/mVenus, mCerulean/Amber, mCerulean/...Other issues that can affect FRET measurements include: the brightness of a fluorophore pair, donor:acceptor...

  16. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...have created synthetic promoters, which typically include some combination of other promoter elements, and...promoters for eukaryotes and prokaryotes We have included two reference tables below listing some of the... simian vacuolating virus 40 Constitutive  May include an enhancer. PGK1 (human or mouse) General expression...

  17. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...integrins are key to a diverse array of processes, including cell adhesion, migration, and signaling, as well...available through Addgene’s repository. The collection includes 26 unique antibodies that bind discrete subunits...domains. All bind specific integrin alpha subunits, including αL, αM and α5. IPI’s anti-integrin rAbs are unconjugated...

  18. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...chloramphenicol-resistant strains, we have also included versions with gentamicin-resistance (amplified...broad group of Gram-negative Enterobacteriaceae including in E. coli O157:H7, Shigella flexneri, Enterobacter...where conjugation may be difficult, we have also included versions of pTOX that encode one of two visible...

  19. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...qualitative and quantitative measurement of processes including RNA movement by tagging RNA sequences with an ...Kellenberger et al., 2013). Other variants of Spinach include Baby Spinach, a miniaturized version of Spinach...aptamer fluorophores, more are in development, including Carrot and Radish from the lab that developed ...

  20. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...have been used to purify a variety of peptides including antibiotic efflux pumps and ankyrin seven-repeat...constructs. Some applications of plasmids from this kit include the production of a suicide switch in cyanobacteria...PUF protein fusions reported in the literature include RNA detection, RNA cleavage, and translational ...
Showing: 281 - 300 of 802 results