... and management process or the whole process, including grant readiness, funding opportunity research,...grant training, mentoring and coaching services, including Smartegrants, to help those entering the Grants...LLC, a grant professional development firm that includes the social media community GrantChat. She has ...

...improvements? You could publish a paper that happens to include your improvements, or maybe even a methods paper...variety of useful molecular cloning protocols, including making LB agar plates, restriction digest of plasmid...been known to recommend protocols listed on OWW, including those for preparing your own competent cells. ...

...scientists employ GFP for a wide number of functions, including: tagging genes for elucidating their expression...detect a variety of intracellular conditions, including ion (such as Ca2+) concentrations and pH, using...selection: Expression constructs like plasmids often include GFP as a marker to help identify which cells have...

...N-glycosylase (UNG) enzymes. Various other proteins included in different CGBE tools allow the researcher to...again, this post has a lot of acronyms, so we've included a glossary at the end of this post! Figure...for their application from among many options.  Conclusion With these advances, every base can now be directly...

...proteins. Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance ...can be used for an array of detectable proteins including fluorescent proteins, antibiotic resistance, and...

... bioinformatics pipelining In many use-cases, including ours at Addgene, the overall purpose of a data...workflow management system for scientific pipelines, including bioinformatics. We loved the parallels between...language.  The modern architecture of Nextflow included built-in support for containers, such as Docker...

...many types of pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA libraries...viruses are sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests specifically...aliquot to full use! Common conditions to optimize include incubation time, antibiotic conditions, and amount...

...previously not associated with ricin regulation, including almost all genes involved in the production of...non-targeting guides and 6,750 safe-targeting guides were included in the library. Safe-targeting guides were designed...activity, Morgens et al drew some generalizable conclusions about off-target activity, demonstrate safe-targeting...

...replication control that are not necessarily mutually exclusive. In the first, the replication initiation factor...Asymmetric plasmid loss occurs for several reasons, including one plasmid blocking replication of another and...incompatibility caused by random assortment of pure plasmid clusters. Molecular Microbiology 56:1430–1440 . https:/...

...rather late stages (i.e. postdoc level) and can include the pressure to publish in high-impact journals...for insights from my time in technical support including tips, advantages, and disadvantages of this overall...without being too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting ...

...If you choose this strategy, it’s important to include the proper protein expression elements (ribosome...entry clones available for many popular genes, including Hs.KRAS4a. Use our website to search for your ...Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression Systems...

...the origin and propagation of the line, and can include assays for authentication and contamination) and...International Cell Line Authentication Committee includes a database listing all cross contaminated or misidentified...working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune, and Infors & Applikon...

...expression strains to accommodate high protein levels including:  ompT: Strains harboring this mutation are deficient...Rhamnose-tunable T7 RNAP expression alleviates inclusion body formation. Expression vector cannot have ...insoluble globs of misfolded protein are known as inclusion bodies, and can be recovered and purified using...

...The first time I heard about FRET during a journal club, my guitarist brain automatically thought about ...and several other pairs have been used since - including: mCerulean/mVenus, mCerulean/Amber, mCerulean/...Other issues that can affect FRET measurements include: the brightness of a fluorophore pair, donor:acceptor...

...have created synthetic promoters, which typically include some combination of other promoter elements, and...promoters for eukaryotes and prokaryotes We have included two reference tables below listing some of the... simian vacuolating virus 40 Constitutive  May include an enhancer. PGK1 (human or mouse) General expression...

...qualitative and quantitative measurement of processes including RNA movement by tagging RNA sequences with an ...Kellenberger et al., 2013). Other variants of Spinach include Baby Spinach, a miniaturized version of Spinach...aptamer fluorophores, more are in development, including Carrot and Radish from the lab that developed ...

...chloramphenicol-resistant strains, we have also included versions with gentamicin-resistance (amplified...broad group of Gram-negative Enterobacteriaceae including in E. coli O157:H7, Shigella flexneri, Enterobacter...where conjugation may be difficult, we have also included versions of pTOX that encode one of two visible...

...integrins are key to a diverse array of processes, including cell adhesion, migration, and signaling, as well...available through Addgene’s repository. The collection includes 26 unique antibodies that bind discrete subunits...domains. All bind specific integrin alpha subunits, including αL, αM and α5. IPI’s anti-integrin rAbs are unconjugated...

...have been used to purify a variety of peptides including antibiotic efflux pumps and ankyrin seven-repeat...constructs. Some applications of plasmids from this kit include the production of a suicide switch in cyanobacteria...PUF protein fusions reported in the literature include RNA detection, RNA cleavage, and translational ...

...resources needed to accomplish your science, and can include: equipment, reagents, animals, glassware, and even...the institution/funding source. Major equipment includes large centrifuges, sequencers and analyzers, and... as many of these tools as possible, this even includes clocks, timers, and phones.  As for reagents and...