... While it is certainly possible to select for desirable traits using traditional plant breeding approaches...modification of known or suspected genes that regulate a desired phenotype. In fact, CRISPR has already been used...wheat, mushroom, and rice (Khatodia et al., 2016). Despite the almost universal functionality of the CRISPR...genes in plants using the same general experimental design principles developed in other model organisms (...nanostructures (Zhang et al., 2019) or cell-penetrating peptides (Santana et al., 2020) and plants viruses (barley... through Addgene. In addition to the plasmids described above, Addgene carries several useful CRISPR toolkits...CRISPR Multiplexing Tools Validate Your Genome Edit Design Your gRNA Additional Resources on Addgene.org ...

...comment reflected the desire for a flexible generic solution, rather than an ELN designed for a specific research...to insufficient and non-standard record keeping despite scientist’s best efforts. We all have software ...practices, their opinions towards ELNs, and their desired functionalities i.e. main priorities of different...was a significant barrier to ELN adoption. This includes financial outlay, staff hours, troubleshooting... to require on-going maintenance and support.” Besides that, academic institutions tend to think about...required for regulatory purposes in many labs. Besides this being of crucial importance when applying ...

...comments section.  FX cloning kit This kit is designed to be used with Type IIS restriction enzymes and...this kit have been used to purify a variety of peptides including antibiotic efflux pumps and ankyrin ...et al. "A transcription blocker isolated from a designed repeat protein combinatorial library by in vivo...followed by leader sequences (encoding small leader peptides) downstream of which you can clone your gene of...Shine-Delgarno sequences used in this so-called bicistronic design (BCD). The 49 vectors in this kit can be used to...(2015): 31-37. pCri system The Gomis-Ruth lab designed the pCri system to simplify the protein purification...for cloning. Furthermore, some plasmids contain peptides to facilitate protein folding and/or protease ...

...CRISPR inhibition (CRISPRi). A) Lentiviral vector design. B) Strategy for leak-free transcriptional control...Organoid EasyTag workflow combines thoughtfully designed plasmid components with Fluorescence Activated... heavy optimization. Claudia Vickers’s lab has designed novel SL fluorescent biosensors by integrating... rather than on the fluorescence intensity, it provides a direct and quantitative detection of SLs. The...biosensors could have a major economic impact.   Design and mechanism of action of the receptor-based SL...targets all mouse genes with 10 gRNAs per gene. The guides are optimized to maximize on-target cleavage and...

... Roth BL (2015) DREADDs (Designer Receptors Exclusively Activated by Designer Drugs): Chemogenetic Tools...less invasive and more flexible as it relies on designer ligands that can be simply introduced by injection...lab and Takafumi Minamimoto’s lab known as deschloroclozapine (DCZ) has been developed to meet these requirements...should be carefully controlled for in experimental design.   Optogenetics Chemogenetics Timing Fast ...Find our chemogenetics and optogenetics science guides ...

...controls and be able to use this information to design thoughtfully and thoroughly controlled experiments...is generally a sample or group that will have a desired response. For example, if you are studying the ...sample, the antibody binds specifically to its desired target (purple) and nonspecifically through the...The Preimmune serum control will not bind to the desired target but will bind nonspecifically through the...the Fc, allowing you to detect this undesirable interaction. When non-specific interactions are suspected...nonspecifically with positively charged groups, such as aldehydes in fixed samples. To address this, block positively... will be a useful starting point for you when designing rigorous experiments. While this blog touched ...

...tagging method that labels cells with heritable barcodes. Barcodes consist of an 8bp variable region and a 6...Direct reprogramming describes the process where differentiated cells are turned into a cell type of ...with the majority of cells failing to develop the desired identity. The development of single cell technologies...Morris Lab at Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously...towards successful iEP reprogramming. MettI7a1 encodes an uncharacterized putative methyltransferase. ...

... been described in this blog. If your data does not meet the assumptions for the tests described above...experimental groups, but how do you determine if they deserve that oh-so coveted asterisk of significance? You...referred to as two-sample or independent, but these designations all refer to the same test. On some occasions...Correction and the Benjamini-Hochberg procedure are designed to reduce false positive rates and control for...error rate than the multiple comparison tests described above. Violated assumptions and statistics Statistical... making your statistical analysis plan when you design your experiment, instead of after you’ve already...

...to a wide range of industries and is a highly desirable quality sought after by employers. These skills...correctly the first time. These skills are very desirable to employers because they save time and money ...Meteorologist Journal Editor Textile Technologist/Designer     Fieldwork and traveling Many people are...employers. Increasingly, computer skills are highly desired, as roles are becoming more reliant on technology...(Python, R, SAS, HTML, Matlab etc) are highly desirable in a range of roles, including: Science Reporter...find answers to include: Will you be sitting at a desk all day or be on your feet constantly in a lab? ...

...or FP) is one of the most important steps in the design of an imaging experiment.  The choice is non-trivial...fusion protein, etc...).  There are many online guides – including an excellent series of posts by Joachim...information about fluorescent proteins.  It was designed with the goal of encouraging user contributions...while also maintaining a relatively strict database design (schema).  Registered users can submit new fluorescent...risk of inaccurate data submissions, so FPbase includes a versioning system that allows users to see when...for, the Table of Fluorescent Protein Properties provides a master list of all proteins in the database,...

... lacks context unless it links to a verified description of that reagent online. Furthermore, if the link... link between the catalog number and reagent description is lost (e.g. a supplier is bought out by another...directly to the original article online. It is also designed to be persistent and exist online indefinitely...specific information about the plasmid: name, description, how the plasmid was generated, validated sequence...authors comply with journal policies, Addgene now provides an RRID and a resolvable compact identifier for...you directly to the lenti-Cas9-VQR plasmid page. Despite their differences, both of these PID formats for...

...pathogen. It includes physical barriers such as the skin and mucosa, antimicrobial peptides and proteins...adaptive immune response is antigen-specific and includes cellular and humoral immunity. Once an adaptive...activate immune components that also lead to the destruction of pathogens. Over the course of an infection...immune response. Over 60 interleukins have been described. NK Cell Signaling Natural killer (NK) cells can... NENF neuron derived neurotrophic factor CIR2, NEUDESIN, SCIRP10, SPUF NGF nerve growth factor (beta polypeptide...immune response. Over 60 interleukins have been described. Symbol Name Synonyms EBI3 Epstein-Barr Virus ...applications in mammalian systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and...

...infectivity): The use of the VSV-G envelope provides the widest tropism or range of cells a virus can infect...The widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted...lentiviral packaging methods. The three components described above (envelope, packaging, and transfer) are ...into the 293T packaging cell line. This system provides the greatest flexibility to pseudotype γ-retrovirus...nomenclature of the plasmid backbone is meant to be descriptive of the components found within the backbone. ...

...is not enough on its own to help you find your desired plasmid. In many cases, you will need a screening...you will need to sort through them to find the desired clone. Do your selection, then screen Why add a...what about identifying clones that contain your desired insert? This is where a  screen comes in. Let’s...blue-white screen, which relies on the lacZ gene. lacZ encodes the enzyme 𝛽-galactosidase which can hydrolyse...Sanger sequencing determines the precise order of nucleotides within a DNA molecule, in this case a plasmid... of the information you need, so you'll want to design a custom primer. Because Sanger sequencing can ...

...created SRIRACCHA in 2017 to enrich and identify desired CRISPR mutants (Wen et al., 2018). SRIRACCHA uses...constructs for the reporter to work and to make the desired genome edit:  A donor plasmid that contains a ...OH-T and doxycycline. A gRNA that targets your desired gene (whose sequence is also found in the reporter...well. The ratio of RFP to GFP expressing cells provides a real-time read out of Cas9 activity.  By using...integrated GFP reporter leaving you with just your desired genomic edit.  Find SRIRACCHA plasmids at Addgene...DSB is repaired by NHEJ which depending on the nucleotides inserted or deleted, can result in an in-frame...

... has precluded widespread adoption of the technology. In 2012, Saito et al. described the first Nano-...molecular and cell biologists over the last two decades, but their use can be limited by a few caveats....hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki et al. improve the brightness of previous...substrate furimazine. In addition to the previously described applications, the enhanced signal of eNL allows...autofluorescence and a high signal-to-noise ratio. Despite the many benefits of Nano-lantern technology, there...

...educational resources — including blog posts, protocols, guides, and collections — is a lot of material to sift... launched earlier in the year, as well as our redesigned homepage and our new search tool, are the results...a few of the strategies we used in our software design. In each case, we leveraged the team’s knowledge...per resource category to expose as filters and designed the search engine to return only the filters that... bits of information for a given resource, and designed the search engine to weight search results differently...'s new search Read to learn more about why we redesigned our homepage and mascot ...

...assessed for the desired parameter. A droplet containing a cell that is positive for a desired parameter is...their observations. However, while flow cytometry provides analysis at the cellular level, it cannot be used...degree of scatter is captured by detectors and provides useful information about the cell. Light that ... traveling is called forward scatter (FSC) and provides information about the relative size of the cell...initially traveling is called side scatter (SSC) and provides information about the cell’s complexity. A cell...side scatter (SSC). Users can gate around their desired cell population for further analysis. In this example...

...into agriculture as species-specific pesticides and insecticides, an advancement over previous chemical...prone to degradation – those are the words that describe RNA! Double stranded? Well, that’s just for DNA...also known as RNAi, are short, dsRNA molecules designed to degrade mRNA transcripts. RNAi activates the...sequence is found (typically RNA that the RNAi is designed against), RISC can repress translation and/or ... and repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular...

... could participate. Despite the group not having a public website, all the slides, handouts, and resources...science or even to discuss potential solutions. Despite all the talk, not much was being done to address...practices could be an actionable way to tackle widespread irreproducibility. Luckily, there were other ...thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman’s vast network...practices could be an actionable way to address the widespread issue of irreproducibility. Use your network...