...transcription factor bind to a particular sequence? Do these two proteins interact? What happens when I ...of modularly assembled zinc fingers. Return to Top Do you have suggestions for other plasmids that should...

... Cas9 orthologs also have distinct PAM sites that do not cross react, allowing multiple Cas9s to be used...have also developed CRISPR editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors...

...protospacer sequence) is G, do not add another G ( e.g., sgRNA-B in Table 4 ) and do not add C to the final...

...influenced by temporally ordered biochemical events. How do RSMs work? The state of an RSM is defined by the ...live, intact cells; the sequencing and qPCR methods do not. How might one implement RSMs? In our study (...that correspond to the desired inputs. Such sensors do not necessarily have to be based on transcriptional...that best suits your application. How efficiently do RSMs work? We tested a two-input (ATc and Ara) RSM...

...prepared me that those challenges would be from doing a remote internship during a pandemic. Before I ...role in the company. One of the things I wanted to do was create content that was unique but also brand...

... job descriptions clearly describe that the roles do not involve bench research. I am always surprised... 5) Apply for the job as requested in the posting Do this online and/or via email exactly as the job posting...

...received over 2,000 entries. Do you mix your passion for art and science? Do you look for the beauty in ...

...and tell your audience just that. Explain what you do know about a related topic, and let them know while...overwhelming than stepping into a sea of posters – where do you begin? Design a game plan Prior to attending ...poster you want to know more about? Interested in doing a postdoc in a presenter’s lab? Want to collaborate...

...it. Biotech companies are known to have tools that do this internally, but they are not available publicly...advised, there is little detail published on how to do it best. Scientists are typically advised to mutate...boosted our productivity in the Biohub. We hope it will do the same for you. Send us your questions or comments...

...blue to ultraviolet light, whereas chromoproteins do not fluoresce at all or fluoresce at very low levels...identified in sea anemone (Chan et al., 2006). Why do these organisms have chromoproteins? One reason may... mutation and excision of the vector backbone. To do this, rhamnose is added which induces expression ...

...Accuracy needed Do you need to call exact copy numbers or just general trends? How confident do you need to...researchers can use to quantify said copies. They do so using copy number variation (CNV) assays. There...

... on the inside of the cell. However, assuming you do need to permeabilize, you have a few options for ...organellar membranes. Created with BioRender.   How do I choose?! Ok, so now you know some of the possible...possible types of fixatives or permeabilizers, but how do you decide which ones to use? Here are some tips ...

...which are doing important work in our microbiomes. Given that viruses are everywhere and doing everything...we try to use them in an experiment and they don't do anything. Viruses are complicated and they perform...

...before using them to screen colonies. The best way to do this is by using your vector with and without an ...clones for later use. There are a few ways you can do this. If you are going to complete your colony PCR...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about ...

...times).     I guess it's cool to be popular, but how do I know if a plasmid is actually useful to me? Plasmid... I want to use this plasmid in a publication, how do I know who to acknowledge? Depositing lab: The name...Our Walkthrough of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene....

...questions about scientists on Twitter: who are they? What do they share? And how they are connected? Here are ... (left), life (center), and physical (right). How do you use Twitter as a scientist? If you don’t use ...

...at Addgene. You are the reason we’re able to do what we do!          Special Thanks Trophies and engraving...

...disease. Figure 1: Scientists are continuing to do essential research into COVID-19 (like here at Addgene...boundaries. To be able to beat this virus, we will need to do the same with science,” said Guillaume Bourque, a...

...shadowing members of my team to learn what I was to do for my time at Addgene. This training prepared me...where each team gives a presentation on what they do and how it relates to other teams at the company....

...publications relevant to your research interests. We do this by promoting citation, supporting sophisticated...can put straight into your reference section. Why do this? Not only does citing plasmids from Addgene ...