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...which are doing important work in our microbiomes. Given that viruses are everywhere and doing everything...we try to use them in an experiment and they don't do anything. Viruses are complicated and they perform...

...before using them to screen colonies. The best way to do this is by using your vector with and without an ...clones for later use. There are a few ways you can do this. If you are going to complete your colony PCR...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about ...

...times).     I guess it's cool to be popular, but how do I know if a plasmid is actually useful to me? Plasmid... I want to use this plasmid in a publication, how do I know who to acknowledge? Depositing lab: The name...Our Walkthrough of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene....

...comes from. Then consider the following questions: Do you need polyclonal or monoclonal antibodies? Will...direct or indirect detection method? If indirect, do you already have secondary antibodies available in...have identical features and epitopes, to ensure you do not validate the same antibody multiple times. You...antibodies will also have a source species. What conjugate do you want to use? Most secondary antibodies will be...buy small aliquots of many different antibodies and do batch validation up front rather than risk delaying...

...disease. Figure 1: Scientists are continuing to do essential research into COVID-19 (like here at Addgene...boundaries. To be able to beat this virus, we will need to do the same with science,” said Guillaume Bourque, a...

...shadowing members of my team to learn what I was to do for my time at Addgene. This training prepared me...where each team gives a presentation on what they do and how it relates to other teams at the company....

...questions about scientists on Twitter: who are they? What do they share? And how they are connected? Here are ... (left), life (center), and physical (right). How do you use Twitter as a scientist? If you don’t use ...

...deposit had already moved on to their next position. We do routinely review deposits that are left in the hold...materials the way we will with journal articles. But we do encourage you to consider sharing them openly as ...

...at Addgene. You are the reason we’re able to do what we do!          Special Thanks Trophies and engraving...

...publications relevant to your research interests. We do this by promoting citation, supporting sophisticated...can put straight into your reference section. Why do this? Not only does citing plasmids from Addgene ...

...you may not have the chance to stop what you’re doing and make a phone call or write out an email. You...which you can describe your issue and press send. Doing so will bring your concern directly to our support...

...education? Let us know in the comments section below! Do you know of a society or event which would benefit...While we are not able to help with every request, we do aim to support events and scientific community activities...

...using microfluidic devices. Addgene: What other uses do you foresee for this tool? Howarth: Polyproteins ...infected with malaria or other parasites. Addgene: Do you have any particular tips or tricks for those ...

...and institutions are not aware of what Addgene can do for them and how the repository can help them share...other side of the force and as an Addgenie I will do all my best to help them in their tasks. But I won...

...experiments and why? What techniques and skills do you need to use to reach these milestones? Can you...milestones along the way so that you don't get stuck doing the same experiment for too long. Keep evaluating...

... yourself with this passion. “Love what you are doing!” It helps when the time comes to work with full...become more aware of careers outside of academia. How do PIs assist their mentees who have goals outside of...

...: Apply library Apply selection Most cells die or do not pass selection (in the case of a reporter) Sort... screen, the goal is to identify those cells that do not survive the selection mechanism. You’ll infect...

...CRISPR could cut DNA, the second generation could do base editing, and with the third generation came ...? The AbuGoot lab realized that they could likely do it using Prime editing alongside some creative (and...represent a major step forward for what the field can do.    References and Resources References  Özcan A...integrases.   Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells...

...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...staining Can be expensive Nanobodies   Recombinant; do not require animal sacrifice Good tissue penetration...Unlike large secondary antibodies, small nanobodies do not oligomerize with primary antibodies when incubated...

... out of the park. It’s clear that you know how to do the job. You may think this means you’re guaranteed...to thrive at the company and add value, not just “do the job”. When they ask about your creativity, your...good but focused on all the other things you have to do. Pause! Enjoy that feedback, bask in the glow of ...