We narrowed to 657 results for: EPO

... fields, including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor have been...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...

...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...bacterium. Plasmids containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent ...transcription factor to bind to a small molecule, the reporter plasmid can be used to detect the presence of ...it. Bacterial one-hybrid (B1H) systems also use reporter genes to identify interactions between proteins...

...s take a look at a few examples with plasmids deposited at Addgene.  PRESTO-TANGO: The ligands that activate...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ...

...off for the day. 1:45 PM--Write final report. Work on a final report for a user research project I led. ...

...activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate...control protein activity Read about RANbodies, reporter nanobody fusions Learn more about using the secondary...

...To make luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using...

... information on each cancer clinical sequencing report includes rare variants that haven’t been characterized...MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319. PubMed Central PMCID...

...antibodies expressed from plasmids that have been deposited with Addgene. Here, we take a closer look at the... VH and VL sequence. These plasmids have been deposited with Addgene for distribution to the scientific...

...populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ...Purification Protocol Video rAbs in the Addgene repository...

...ability to infect cells with a high efficiency and deposit a DNA/RNA cargo into their targets makes them attractive...Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...

... get started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about what makes...make your mentor want to keep working with you is report back with progress. Tell them how their advice ...

...catalog would allow us to enhance our biosensor repository as we find ourselves lacking several crucial ...open science, Hébert plans on building upon and depositing their modified constructs to Addgene. Congratulations...

...differentiated and quiescent it is, and the more depolarized (positive) the cell is, the more embryonic, stem...technologies are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators...

...Ramanathan et al. also used the Contamination Repository for Affinity Purification (CRAPome), to identify...identify highly biotinylated background proteins reported by other research groups that performed BirA*-based...

... be noted that the photostability measurements reported in the literature are performed in different ways...information can help guide your understanding of reported data. Photobleaching results in a loss of fluorescence...

...research.   Abhi Aggarwal: Glutamate sensing reporters Abhi Aggarwal is a postbaccalaureate research ...third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule...

...Many of these tools can be found in Addgene's repository (Table 1). Figure 2: Adding function to... (with rHUH). Many of these tools are already deposited at Addgene in multiple formats: for use in different...

...workforce working group report. http://acd.od.nih.gov/biomedical_research_wgreport.pdf Resources at Addgene...

...are easy with CRISPR. Addgene: You’ve made many deposits to Addgene over the years. Can you describe the...will be available on GitHub, an open source code repository. Addgene: Over the longer term, where is this...

...the development of a vast number expression and reporter plasmids and the free exchange of these vectors...characterization of NRs and coregulators, and although the reporter gene has over the years evolved from chloramphenicol...