We narrowed to 650 results for: EPO
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TypeBlog Post...catalog would allow us to enhance our biosensor repository as we find ourselves lacking several crucial ...open science, Hébert plans on building upon and depositing their modified constructs to Addgene. Congratulations...
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Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post... be noted that the photostability measurements reported in the literature are performed in different ways...information can help guide your understanding of reported data. Photobleaching results in a loss of fluorescence... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...research. Abhi Aggarwal: Glutamate sensing reporters Abhi Aggarwal is a postbaccalaureate research ...third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...differentiated and quiescent it is, and the more depolarized (positive) the cell is, the more embryonic, stem...technologies are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators... -
The Future of Research Symposium Boston 2015
TypeBlog Post...workforce working group report. http://acd.od.nih.gov/biomedical_research_wgreport.pdf Resources at Addgene... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...are easy with CRISPR. Addgene: You’ve made many deposits to Addgene over the years. Can you describe the...will be available on GitHub, an open source code repository. Addgene: Over the longer term, where is this... -
Working with Nuclear Receptors
TypeBlog Post...the development of a vast number expression and reporter plasmids and the free exchange of these vectors...characterization of NRs and coregulators, and although the reporter gene has over the years evolved from chloramphenicol... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...Associate Professor at Boston College. National reports stress the importance of providing authentic research...Students have used these plasmids, which have been deposited with Addgene, to successfully analyze Met protein... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post... (abundant in eukaryotes) A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein Interactions...green-yellow or orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...researchers to develop and deposit the many plasmid tools available in the Addgene repository. To learn more about... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...such as the serotonin 3 receptor, will promote depolarization in neurons. Alternatively, combining with the...DREADD-based chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus... -
Viral Vectors 101: Virus Safety
TypeBlog Post...they are integrating viruses – they physically deposit their cargo into the host’s DNA. These viruses ...familiarize yourself with your organization’s reporting mechanisms before you start an experiment, just... -
Tips for Technical Support Calls
TypeBlog Post...the experiments performed by our thousands of depositors. That means their original articles are your ... Addgene Help Center Get Help with the Addgene Deposit Process Resources on Addgene.org Check Out Our... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog Post...supervisor might expect weekly or biweekly progress reports. However, a hands-off busy supervisor might get...update, and whether he or she would like a written report or to meet in person. Regardless of your supervisor... -
3D Printing Meets CRISPR Cas9
TypeBlog Post... nuclease active site wasn’t even close to its reported cleavage site on the complementary DNA strand....model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce off-... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post..., which takes about one week from the initial deposition of the fertilized egg by its mother. Inside the...Drosophila Plamids Drosophila CRISPR Resources Deposit Your Drosophila Plasmids ... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...sections of DNA can be more difficult. Addgene depositors have developed new CRISPR-based methods for various...or cell lines, please consider sharing them by depositing with Addgene! Figures were adapted from: Hall... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...