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...are coming along at a fast and furious pace. In December, as we've blogged about before, there was the ...via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos. Niu Y, et al. Cell. 2014 January 30. (Pubmed) ...

...gRNA plasmids, including: Multiplex CRISPR/Cas9 Assembly Systems Multiple Lentiviral Expression System ...CRISPR Blogs CasPEDIA Content last reviewed: 19 December 2025 Do you have suggestions for other plasmids...

...how it's used. Chapter 12: Darrell's goals as a member of the Addgene board of directors - increasing ...stick to our core mission. Chapter 13: Outro - remember to step back and ask, "Why did I start doing this...

...genome editing plasmids String assembly gRNA cloning (STAgR) String assembly gRNA cloning (STAgR), developed...

...to specific drugs in specific contexts, so he assembled a plasmid kit designed to do exactly that. As ... described in the journal Science Signaling in December 2014, he and his colleagues at Duke University...

... former Co-director of Science in the News, and member of the SITN podcast team. You can follow Vini on...former Co-director of Science in the News, and is a member of the SITN podcast team. You can follow Amy on...

...algae cell is just the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in...appropriately named as spook, spookier, phantom, disembodied, shadow, and shade. Tongue-eating louse Cymothoa...

...immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-CHIME and S-CHIME...

...create Phage Directory in a whirlwind three days in November 2017. #Phage researchers! I am working with...Strathdee, 🗡️Superbug Slayer 🗡️ (@chngin_the_wrld) November 8, 2017 Facilitating the search of phages for...

...benefits for both PI and trainee. DePace and her lab members have noticed that the meetings make the lab run...the PI has an opportunity to check in with lab members, determine how to work through obstacles, and take...

...all of the Addgene employees, the Addgene board members, vendors, Addgene supporters, and of course, Executive...integral part in its success. The Addgene board members were also recognized for their essential advice...

...filled with family, food, and travel, and you can’t remember where you put your flow cytometry protocol. Or...Tip 3: Share protocols and spreadsheets with lab members Now that you’ve organized your protocols, wouldn...

...participate in a brief fifteen minute interview. I remember leaving the interview hopeful to continue on in...the beginning, I spent a lot of time shadowing members of my team to learn what I was to do for my time...

...Addgene’s Lianna Swanson, who has been working with members of Davidson's lab to coordinate the impressively...F-Actin to mitochondria, from the nucleus to cell membrane, this collection has fluorescently tagged versions...

...adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction Bunz Return to top Adenoviral...using the AdEasy™ System Content last reviewed: 12 November 2025 Do you have suggestions for other plasmids...

...for the first time, being introduced to all the members around the office one by one, I knew that I belonged...right concentration of a reagent during our HiFi assembly but decided to go ahead anyway. We were running...

...The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended...Llamas Required - Synthetic Nanobodies Against Membrane Proteins More resources from addgene.org  Plasmids...

...performed with a probe surrounded by a semipermeable membrane, which is inserted into the region of the brain...passive diffusion of DA across the semipermeable membrane from the extracellular fluid into the perfused...

...checklist as champagne or cupcakes, or maybe the lab member originally responsible for the deposit had already...workflows and keep things moving internally. And remember, a publication isn’t required to share your plasmids...

...insights they glean from a reading session. To remember what happened when drug X was added to cell line...with a specific gene knock out, often you have to remember the experiment, not the paper. To get around reading...