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We narrowed to 801 results for: Eng

Showing: 281 - 300 of 801 results

  1. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...soluble and insoluble proteins, which can be a challenge for classic antibody based affinity purification...for your proximity labeling experiment! APEX: engineered ascorbate peroxidase that can rapidly biotin ...available, the Alice Ting Lab demonstrated that engineered ascorbate peroxidase (APEX), used as a genetic...Ramanathan et al., 2018). To develop RapID, scientists engineered a mutant BirA* from B. subtilis, termed BASU....PMCID: PMC4296904. Martell, Jeffrey D., et al. "Engineered ascorbate peroxidase as a genetically encoded...

  2. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...BIOFAB Plasmid Set contain terminators of varying strengths centered within GFP/RFP bicistronic reporters ...required for eukaryotic systems and instead directly engages the ribosome for translational complex assembly...populations through time, Christopher Marx's lab has engineered and validated a new method called FREQ-Seq. This...proteins are composed of repeat segments that can be engineered to bind specific RNA bases. By swapping out these...2016): 895-899. Choudhury, Rajarshi, et al. "Engineering RNA endonucleases with customized sequence specificities...

  3. Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!

    Type
    Blog Post
    ...Regulating telomere length with TZAP. Listen to the segment on regulating telomere length with TZAP Hot Plasmid...tools for plants. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  4. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...🎄🎁🎅🤶🎄 pic.twitter.com/rDaEjxTNZX— Heng Chooi (@YitHengChooi) December 4, 2021   3rd Place: O! Beaker... Though 2021 had more than its fair share of challenges, the annual Deck the Lab Contest brought us smiles...Falcon Tube created by the team efforts of the Yit Heng Chooi Lab at the University of Western Australia...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method that harnesses the...template. Although effective, ivTRT may be more challenging for sequences with complex secondary structure... systems (ttA or rtTA) are between 1 to 2 kb in length. There are plenty of Addgene plasmid options for...conditional alleles by CRISPR/Cas-mediated genome engineering." Cell 154 (2013):1370-9. DOI: 10.1016/j.cell... a robust method for in situ germline genome engineering using CRISPR nucleases. Genome Biol. 19 (2018...

  6. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...  We’ll look at examples of both natural and engineered repressible promoters. Chemically repressible...transactivator components of the system have been engineered and improved. Takara’s “2nd generation Tet-Off...system is a positive repressible promoter, and was engineered from the cmt and cym bacterial operons. A chimeric... and repress pADH1 activity. Researchers have engineered pADH1 variants that are less sensitive to ethanol...Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS...

  7. Finding Your Perfect Job After University

    Type
    Blog Post
    ...become bored because I was not being mentally challenged. For these and other reasons, I started  looking...tuition fees had just gone up dramatically and, in England, you could not receive government loans to study...reason why I chose Australia was because it is an English speaking country with a strong economy and plenty...combination of genetic mutations. This is a particular challenge in pancreatic cancer where one drug does not work...while I’m based in London) does bring its own challenges. I’ve had to become skilled with technology like...

  8. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...PhD, achieving our career goals can be a real challenge. On top of that, in the midst of a rapidly changing...mentees with a broader, first-hand vision of the challenges and career opportunities that truly exist outside...was much more than that. I discovered that the challenges that I am facing in my career are common problems... that many others have faced yet, still, this challenge did not hinder them from advancing their careers...mentoring program extends its scope to the fields of Engineering, Economics, and Philology (in addition to Biomedicine...

  9. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...University of Malawi where he focused on agricultural engineering through biotech. After graduating with a masters...Kingdom's PhD projects - HIV inhibitors, biofuels, challenges in Malawi, and more 23:45-28:07 : How Seeding...Seeding Labs here. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  10. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD we've had on the podcast...The Wyss Institute for Biologically Inspired Engineering and why Alex decided to work there. 13:22 - 22...of DNA and RNA.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  11. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...Alternatively, endogenous miRNA precursors can be engineered to created artificial miRNAs. The processed miRNA...frequently used. Although similar, amiRNAs are engineered from endogenous precursors of microRNAs found...specific than long hpRNAs, they must be individually engineered and empirically evaluated for their gene silencing...using a fluorescent output to monitor RNAi, we engineered a positive selection reporter whereby survival...Gong B-Q, Guo Y, Xu L, Zhang X-N, Li J-F (2018) Engineering Artificial MicroRNAs for Multiplex Gene Silencing...

  12. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...weighted by the position of the mismatch along the length of the gRNA given the stricter sequence requirement...cleavage assays to detect mutations triggered by engineered nucleases." G3: Genes| Genomes| Genetics 5.3 ...23934178. PubMed Central PMCID: PMC3782611. 8. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling...detection of DNA double-stranded breaks induced by engineered nucleases." Nature biotechnology 33.2 (2015):...23503052. PubMed Central PMCID: PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "Defining and improving...

  13. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...and creativity in the face of funding challenges. By strengthening our commitment to collaboration and ...and increased tariffs present an unprecedented challenge to the scientific research ecosystem, not just...years to come. We Are Here to Help Despite these challenges, Addgene remains steadfast in our mission to ...

  14. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ...repair, stem cells, gene therapy, and tissue engineering. How the multidisciplinary nature of the Center... Chapter 6: Near-term possibilities for using engineered cells in therapy. The lungs are difficult, but...Boston Medical Center. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  15. Typing CRISPR Systems

    Type
    Blog Post
    ...2013. Since that point, it has become the most engineered Cas enzyme, with adaptations for increased fidelity...target and degrade select RNA transcripts, or be engineered to conduct direct RNA editing. There are four... L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/CAS systems. Science, 339(6121),...Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng, D., Haft, D. H., Horvath, P., Moineau, S., Mojica... J., Chong, S., Makarova, K. S., Koonin, E. V., Cheng, D. R., & Scott, D. A. (2018). Functionally diverse...

  16. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...simply with the presence or absence of the correct wavelength of light. This response is immediate, on the ...activation to behaviors. Scientists continue to engineer opsins to operate at increasingly faster kinetics...Optogenetics also requires that light of the correct wavelength reach receptors in the brain. One way to address...can be simply introduced by injection. The main challenge in using ligands is ensuring the agonist is able...

  17. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...should possibly take a composition class in the English Department. He suggested that I sign up for the...the SWPI online course because a course in the English department wouldn’t have trained me in the scientific...not prose; it is to be straight to the point. 3. Engage the reader Scientists forget that the reader is...has to introduce the reader to the manuscript and engage them in the material. So the manuscript should ...

  18. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...CASTs quickly became popular bacterial genome engineering tools, with early reports of 40%–60% efficient...catalyzes DNA insertion, along with both evolved and engineered adjustments to the other components. It drove... & Sternberg, S. H. (2024). Bacterial genome engineering using CRISPR-associated transposases. Nature ...the Addgene blog INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Degrading DNA with ...

  19. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...in the past year. Cambridge Biological Society, England Since 1996, Cambridge Biosoc’s goal has been to... up to professors. Oxford Biological Society, England The Biological Society based at the University ...networking events. Imperial Synthetic Biology Society, England The Synthetic Biology Society is a new society ...incredibly glad to see these biological societies engaging with young scholars. We believe it’s very important...

  20. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...the full length plasmid. If you find that your bacterial cultures contain a mix of full length plasmid ...will also outgrow bacteria that contain the full length plasmid.   Figure 1: The full lentiviral...like DH5-alpha are recA-, some strains have been engineered to further reduce recombination. Recombinase-...
Showing: 281 - 300 of 801 results