We narrowed to 372 results for: Fus

1. CRISPR Activation: A Practical Guide

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   ...for the upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity...

2. Tips for CRISPR Gene Editing in Mice

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   ...the microinjection was done as the pronuclei were fusing, you may have several mosaic mice. To figure out...

3. Antibodies 101: Beyond Surface Labeling

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   ...visualizing DNA instead of proteins. Some stains can diffuse through the cell membrane freely, while others ...

4. An Integrin Antibody Toolkit from IPI

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   ...antibodies composed of rat or mouse variable regions fused with rabbit constant domains. All bind specific ...

5. Tips for Getting a Faculty Position

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   Blog Post

   ...answer an expert's question than to leave everyone confused about your research because you waded too deep...

6. Transferable Skills Guide: Conflict Resolution

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   ...taking care of that”, because that can indicate confusion around... Ownership: Misunderstandings about who...

7. Quick Guide to Near-Infrared Fluorescent Proteins

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   ...less interference with the oligomeric state of a fusion partner. Therefore, we recommend using miRFPs and...

8. Adeno-associated Viruses (AAVs) for Genome Editing

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   ...templates for applications that require large gene fusions. The current state-of-the-art in combining the ...

9. The Fluorescent Vegetables in Aptamer Soup

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   ... composed of a “Top” and “Bottom” portion, each fused to a separate piece of RNA. Fluorescence is observed...

10. Five Popular Model Organisms, Part 2

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    ...tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms including Xenopus. ...

11. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    ...available from Addgene. This system uses the dCas9-VP64 fusion commonly used to activate transcription, but with...

12. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post

    ...techniques, such as Golgi staining, injection of diffusible labels, or electron microscopy of stained sections...

13. Antibodies 101: Multiplex Immunofluorescence

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    ... antibody against your target protein, consider fusing a protein tag to the target and using an epitope-based...

14. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    ...actually performed, potentially curtailing future confusion (and hours sifting through paper lab notebooks...

15. Generating Mouse Models Using CRISPR/Cas9

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    ... of the skyline of Acadia National Park. I was confused. It was supposed to be hard. I was mentally prepared...

16. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    ...minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF...

17. Important Considerations When Using AAVs

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    ...axonal transport desirable or not desirable? If diffuse gene expression is tolerated, then axonal transport...

18. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    ...homeostasis maintained through mechanisms like fission, fusion, biogenesis, and mitophagy, can be tracked using...

19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    ...stimulus. Using an epifluorescence microscope and GFP fusion proteins controlled by promoters specific to the...

20. Chemogenetics AAV Preps

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    ...nEF CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity...Inhibition HA fusion none 2, 5 Richie 121539 pAAV SYN1 HA-hM3D(Gq) hM3D(Gq) - Activation HA fusion none 2, ...mCherry fusion none 5 Roth 50479 pAAV-GFAP-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion none 5...-hM3D(Gq)-mCherry hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 44362...-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 65417...pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Roth 50455 pAAV-hSyn-DIO-HA-hM4D...pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Roth 50458 pAAV-hSyn-DIO-rM3D...