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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: GAN

Showing: 281 - 300 of 610 results

  1. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...is reflected in the biology, too. CRISPR is an elegant bacterial immune system and an efficient gene editing...distinguishing the foreign sequences and keeping the array organized. Target strand: The strand of foreign DNA that...

  4. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...The best trap depends on your experiment and the organism you are working in. The spacing of genes and whether...

  6. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ... editing tool of choice for a number of model organisms: mammalian cells, yeast, drosophila, plants, worms...obligate thermophiles without the need to grow the organisms at lower than optimal temperatures. GeoCas9’s ...

  7. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...skills”. Leadership is prized at all levels of an organization and is also one of the most common criteria ...work alone much of the time, depending on the organization they lead. Effective leaders rarely give orders...

  8. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...valued This often stems from being included in organizational decision-making. It is imperative that employees...understand the part they play in the goals of the organization and feel the importance of their contributions...

  9. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...your expression system or environment. The host organism will determine the type of vector that you will...your plasmid has been incorporated into the host organism, usually achieved with the proper selection marker...

  10. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...where they are. 8)   Look into training from an organization such as COMPASS. Although COMPASS has a special... Kendall Morgan...

  14. What the HEK?

    Type
    Blog Post
    ... etc.) and are ready to use on another cell or organism. This pipeline is utilized to transduce cell lines...resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral...

  15. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... 5’ of the gene they control. Promoters can be organism or tissue type-specific, which can help you restrict....2019.00093 Wilmott P, Lisowski L, Alexander IE, Logan GJ (2019) A User’s Guide to the Inverted Terminal...

  20. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...tools by increasing gRNA stability. First, they began with the Broccoli aptamer system to visualize the... will recruit the HaloTag with red fluorescent ligands to the loci of interest. Dual color detection In...
Showing: 281 - 300 of 610 results