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We narrowed to 1,144 results for: Gene

Showing: 281 - 300 of 1144 results

  1. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...explains how gene drives work and what work he's planning on doing with CRISPR-based gene drives focusing...developing new CRISPR tools, and the potential uses of gene drives. Listen here! Podcast breakdown (Time : ...- 31:25 : Nature's defenses against gene drives. 31:26 - 33:08 : Plans for future work at the Wyss Institute... gene drives...became a member of the Addgene advisory board where he will help shape Addgene's future. Listen to this...member of the Addgene Scientific Advisory Board with a particular interest in generating Cas9 based tools...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  2. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...lifecycle. Halloween genes The Halloween genes are a set of cytochrome P450 genes discovered in Drosophila...Mutations in these genes are lethal, associated with defective exoskeleton formations. These genes were appropriately...1983): 813-816. Gilbert, Lawrence I. "Halloween genes encode P450 enzymes that mediate steroid hormone...We’ve had teams dressed up as plasmids, Blugene, and the Addgene ping pong table. This year, in addition...Additional resources on the Addgene blog Read about company culture at Addgene See some of our previous ...costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways to incorporate science themes...Halloween contest, we’ve also carved pumpkins (yes, Blugene made an appearance there too) and shared fascinatingly...

  3. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...mixture of XFP genes. Stochastic multicolor labeling is possible when each of the XFP genes is introduced...fluorescent protein (XFP) genes in a transgene. When multiple copies of the transgene cassette are introduced...result in a combinatorial expression of these three genes with different copy numbers, producing dozens of...modern neuroscience, plasmid or viral vector-mediated gene delivery is more common than conventional transgenic...transgenic strategies. In such vector-based gene delivery systems, the stochastic expression of XFPs is possible... Plasmid concentrations and AAV titer - The XFP genes have to be introduced at ~2 copies per cell per ...plasmids (Addgene #104102 - #104105) using in utero electroporation or with AAVs (prepared with Addgene #104110...

  4. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...zebrafish genes; many of these were generated as part of an NIH-sponsored effort to provide gene-editing...zebrafish genes. TALengineering... TALengineering Reagents TALEN Zebrafish Reagents TALENs for Endogenous Zebrafish Gene Targets...gene-editing nucleases to the zebrafish community. Target Gene Name TALENs Full Target Site (5' to 3'; half-sites...

  5. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...knockout of a target gene and expression of a separate gene. In these mouse lines, gene knockout can be induced... type specific) control of DNA recombination and gene expression can be advanced by the coordinated use...recognizes FRT sites and initiates site directed gene recombination between those sites. FRT sites and...sequence of interest can lead to poor expression of genes within that sequence and should therefore be prevented...recombination of the loxP sites and thereby to the gene knockout. In this experiment it is possible to compare...morphology and synaptic connectivity before and after the gene knockout in the same mouse.   Scientists always...researcher exquisite control over expression of multiple genes in specific cells at specific times. Many thanks...

  6. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...varies across targets, they tested two genes in mice. For both genes, they saw indel formation and phenotypic...in vitro and germline genome editing, but in vivo gene editing approaches have been limited by problems...viral vectors (AAV) are commonly used for in vivo gene delivery due to their low immunogenicity and range...al. successfully used CjCas9 with AAV to target genes in mouse muscle and eye tissue. Neisseria meningitidis...cell is transduced by both AAVs, the full length gene and/or protein is reconstituted. One way to reconstitute...split intein spCas9-AAV toolbox that retains the gene-targeting capabilities of full-length SpCas9. This... of plasmids includes AAV-Cas9C-VPR for targeted gene activation. Split inteins have also been used to...

  7. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ...CRISPR reagents and other specific gene sequences available from Addgene. Dr. McMurray hopes that by demonstrating...lab has generated mice wherein it's possible to delete the functional portion of the Nfix gene using Cre...phosphomannomutase (PMM) activity due to mutations in the PMM2 gene. Videira’s group recently reviewed and reported ...interactions and how these interactions regulation gene expression. Recent studies have shown that the conditions... Smith Syndrome are due to mutations in the NFIX gene. These diseases are characterized by overgrowth ...studying the function of the mouse Nfia, Nfib and Nfix genes in brain development since 2000 and have shown several...NFIX function. They will then mutagenize the NFIX gene and test whether variants of unknown significance...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...understanding of B. subtilis gene functions and interactions. The single-gene deletion library is derived...studying the phenotype of an essential gene, the CRISPRi Essential gene Knockdown library was constructed ...Bacillus subtilis libraries with Addgene – the B. subtilis Single Gene Deletion Library—Kanamycin and the...compatible cloning sites for easy cloning of your genes of interest. In order to improve the study of specific...cassette. However, it was unknown whether variations in gene expression from these polycistronic cassettes could... stoichiometry impacted reprogramming and global gene expression patterns. Polycistronic cassettes that... the CRISPRi Essential gene Knockdown library. Although B. subtilis has a fully sequenced genome, many...

  9. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...possible deletion strategies for genes and non-coding elements. For creating a gene knockout, two sgRNA located...evaluate the RNA, perform RT-qPCR for gene expression of the relevant gene 7,8 . To evaluate the protein, perform...genome editing tool that allows genetic perturbation of genes and genetic elements. Here we present a simple...for efficient loss-of-function studies of genes and genetic elements in mammalian cell lines. Protocol...theory and methods, perspectives for drug delivery, gene therapy and research. Gehl J. Acta Physiologica...Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud to present... be utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include...

  10. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...found in the same gene. If we only have small, directed editing abilities, any gene editing treatment ...functional copy of the entire gene, allowing a one-size-fits-all approach to gene replacement therapy for most...insertions, leaving a several kilobase-sized gap in the gene editing field. The AbuGoot lab has now developed...to work. (It was also found to be independent of gene repair.) This lowers the chance of the insert being...activity. They now had a system that could integrate genes up to 36 kb in length, with an integration rate ...system.     Fig. 2: Schematic of programmable gene insertion with PASTE. The PASTEsystem involves insertion...primary cells, and can be easily programmed for new genes. In comparison with other large insert techniques...

  11. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...Versatile Viral Tool for Gene Expression in Mammals Additional Resources on Addgene.org Find Ready-to-Use ...the cell on their way to the nucleus where their gene cargoes can be expressed. The bottom line is that...successful transduction. In another example, neuronal gene expression by an anterograde AAV was shown to drop...cell, you may not be able to detect AAV-mediated gene expression in a target cell. Practical considerations...experimental setup. Some experiments require higher gene expression and therefore higher titer than others... to such a high titer when looking for effective gene expression. How do you achieve high titers in the...therapy : the journal of the American Society of Gene Therapy. 2008;16(7):1189-1199. doi:10.1038/mt.2008.103...

  12. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...EGFP can be used as positive controls for gene editing or gene activation, while the BRAF and MAP3K4 gRNAs...provide the Brunello (targeting human genes) and Brie (targeting mouse genes) pooled libraries from the labs...editing. You can also enhance expression of your gene of interest with a variety of Cas9 activators available...pooled gRNA libraries that can be used to screen for genes involved in a wide variety of cellular processes...80,000 gRNAs to target around 20,000 individual genes. We provide enough lentivirus for you to both perform...and member of the Addgene Advisory Board) says: "When you're setting up to do a genetic screen, you need... are members of the Addgene Advisory Board. Additional Resources on the Addgene Blog Get the Most from...

  13. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...alternative method for generating transgenic mice strains and for non-invasive in vivo gene editing. The bottom...repression, transgenic mouse line creation, and in vivo gene editing of mice, with the potential to be used for... Primary cells are often refractory to gene delivery methods, but the researchers was able to edit hiPSCs... stimulation, respectively. Primary cell type Gene targeted by guide Editing efficiency hiPSCs  EMX1...cells when using a gRNA targeting the Fto gene, while editing was not detected in cells electroporated with... used Nanoblades to disrupt the tyrosinase (Tyr) gene in mouse zygotes, which results in albinism in mice....  Nanoblades can also be used to non-invasively gene edit the liver of adult mice. When mice were IV ...

  14. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...toolkits available at Addgene.  C. elegans open resources The C. elegans Neuronal Gene Expression Map & Network...expression of your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire...-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans." Genetics 202.4 (2016): 1277-1288... The CeNGEN goal is to establish a comprehensive gene expression atlas of every C.elegans  neuron. Scientists... C. elegans embryo when all essential embryonic genes are individually depleted via RNA interference (...based bipartite expression system that can regulate gene expression in C.elegans. In this system a promoter...that can bind to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In ...

  15. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...Plasmids designed to express genes in a given host cell type are generally broken down into two broad categories...discussed the promoter's role in the initiation step of gene transcription; today we'll provide an overview on...define the end of a transcriptional unit (such as a gene) and initiate the process of releasing the newly...machinery. Terminators are found downstream of the gene to be transcribed, and typically occur directly ...studies focus on promoter strength as a determinant of gene expression levels, the terminator also plays an ... to variability in RNA half-life, and ultimately gene-expression. Polyadenylation is the post-transcriptional... in a Promoter-Dependent Manner. Hum Gene Ther. 2008. PMID: 18627247 Zufferey R, Donello JE, Trono D, ...

  16. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...Repair is Used to Edit Genes Read How Non-Homologous End Joining is Used to Delete Genes Additional Resources... CRISPR gene therapy, it’s important to realize that DMD is a simpler case than other genetic diseases...there is still no good treatment. The dystrophin gene is very large (79 exons), but much of the sequence...Dystrophin’s size makes it difficult to deliver via gene therapy, so researchers have set their sights on... cells expressed functional dystrophin. Editing genes in vivo is of course much more difficult than in...cells, where precision editing of the dystrophin gene would take place, with minimal risk of off-target...clinically with CRISPR. Many obstacles remain for CRISPR gene editing, but given the speed at which the technology...

  17. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...through this challenging sequence. Transgene The transgene is the gene that will be delivered by the AAV..., or your gene of interest. Promoter The promoter drives the expression of the AAV transgene. On a plasmid...plasmid which contains the structural and packaging genes, the adenoviral helper plasmid which contains the...plasmid map, promoters are upstream or 5’ of the gene they control. Promoters can be organism or tissue...for the successful and specific expression of your gene of interest. References Haery L, Deverman BE, ...Terminal Repeats of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089...control transgene expression. Lox sites are directional 34 bp sequences that can flank the transgene and ...

  18. RUBY-Red Siliques

    Type
    Blog Post
    ...fluorescent proteins. Selection using resistance genes usually involves killing the untransformed seedlings...individual. For example, with CRISPR-Cas9 gene editing, transformation may be identified by resistance ...continued production of new alleles or off-target gene editing. Antibiotic selection would only then kill...requires specialized equipment. Reporters for studying gene expression have similar drawbacks, often requiring...Res. (2020).   I can do this by expressing a gene that rescues the mutations, using RUBY as a selection.... et al. A reporter for noninvasively monitoring gene expression and plant transformation. Hortic Res ...presence of a transgene instead, which can’t be done if your selection method kills the transgene free individual...

  19. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...repression of your target gene, activation and repression of different genes in a single cell, and localization...replication and transcription as well as reporter genes. To learn how you can use minigenomes in your research... them in action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated viruses (... (AAVs) are commonly used to deliver a genes of interest to a variety of cell types in-vivo. These are...localization of a fluorescent signal to your target gene. Read this post to find out more about how the Casilio...are widely used by the optogenetics and chemogenetics communities to activate neurons and neuronal pathways...guest writers. Your contributions make Addgene and the Addgene Blog useful resources for the biological...

  20. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...cross-cassette interactions in gene expression, and to generate multi-gene knockouts. Mammalian and fly ... to generate anything from individual transcription units (TUs) used to express your favorite gene-of-...While a generic MoClo system for any given species can be repurposed for CRISPR applications (gene insert...DNA assembly tool used to build single- and multi-gene constructs for a variety of applications. MoClo ...several additional capabilities, including conditional gene expression, multiplexed CRISPR, and chromosomal ...). Those looking to express high levels of their gene of interest in yeast should also take a look at ...elements for S. cerevisiae chromosomal integration and gene expression, the system includes a toolkit to express...
Showing: 281 - 300 of 1144 results