Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 1,140 results for: Gene

Showing: 281 - 300 of 1140 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...found in the same gene. If we only have small, directed editing abilities, any gene editing treatment ...functional copy of the entire gene, allowing a one-size-fits-all approach to gene replacement therapy for most...insertions, leaving a several kilobase-sized gap in the gene editing field. The AbuGoot lab has now developed...to work. (It was also found to be independent of gene repair.) This lowers the chance of the insert being...activity. They now had a system that could integrate genes up to 36 kb in length, with an integration rate ...system.     Fig. 2: Schematic of programmable gene insertion with PASTE. The PASTEsystem involves insertion...primary cells, and can be easily programmed for new genes. In comparison with other large insert techniques...

  2. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...Versatile Viral Tool for Gene Expression in Mammals Additional Resources on Addgene.org Find Ready-to-Use ...the cell on their way to the nucleus where their gene cargoes can be expressed. The bottom line is that...successful transduction. In another example, neuronal gene expression by an anterograde AAV was shown to drop...cell, you may not be able to detect AAV-mediated gene expression in a target cell. Practical considerations...experimental setup. Some experiments require higher gene expression and therefore higher titer than others... to such a high titer when looking for effective gene expression. How do you achieve high titers in the...therapy : the journal of the American Society of Gene Therapy. 2008;16(7):1189-1199. doi:10.1038/mt.2008.103...

  3. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...EGFP can be used as positive controls for gene editing or gene activation, while the BRAF and MAP3K4 gRNAs...provide the Brunello (targeting human genes) and Brie (targeting mouse genes) pooled libraries from the labs...editing. You can also enhance expression of your gene of interest with a variety of Cas9 activators available...pooled gRNA libraries that can be used to screen for genes involved in a wide variety of cellular processes...80,000 gRNAs to target around 20,000 individual genes. We provide enough lentivirus for you to both perform...and member of the Addgene Advisory Board) says: "When you're setting up to do a genetic screen, you need... are members of the Addgene Advisory Board. Additional Resources on the Addgene Blog Get the Most from...

  4. Synthetic Biology - Sensing and Signaling

    Type
    Collection
    ...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter Tags PI Publication ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  5. Synthetic Biology - Strains

    Type
    Collection
    ...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...available from this depositor's lab. Plasmid Description Gene/Insert Antibiotic Resistance PI Publication Back...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  6. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...alternative method for generating transgenic mice strains and for non-invasive in vivo gene editing. The bottom...repression, transgenic mouse line creation, and in vivo gene editing of mice, with the potential to be used for... Primary cells are often refractory to gene delivery methods, but the researchers was able to edit hiPSCs... stimulation, respectively. Primary cell type Gene targeted by guide Editing efficiency hiPSCs  EMX1...cells when using a gRNA targeting the Fto gene, while editing was not detected in cells electroporated with... used Nanoblades to disrupt the tyrosinase (Tyr) gene in mouse zygotes, which results in albinism in mice....  Nanoblades can also be used to non-invasively gene edit the liver of adult mice. When mice were IV ...

  7. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...toolkits available at Addgene.  C. elegans open resources The C. elegans Neuronal Gene Expression Map & Network...expression of your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire...-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans." Genetics 202.4 (2016): 1277-1288... The CeNGEN goal is to establish a comprehensive gene expression atlas of every C.elegans  neuron. Scientists... C. elegans embryo when all essential embryonic genes are individually depleted via RNA interference (...based bipartite expression system that can regulate gene expression in C.elegans. In this system a promoter...that can bind to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In ...

  8. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...Plasmids designed to express genes in a given host cell type are generally broken down into two broad categories...discussed the promoter's role in the initiation step of gene transcription; today we'll provide an overview on...define the end of a transcriptional unit (such as a gene) and initiate the process of releasing the newly...machinery. Terminators are found downstream of the gene to be transcribed, and typically occur directly ...studies focus on promoter strength as a determinant of gene expression levels, the terminator also plays an ... to variability in RNA half-life, and ultimately gene-expression. Polyadenylation is the post-transcriptional... in a Promoter-Dependent Manner. Hum Gene Ther. 2008. PMID: 18627247 Zufferey R, Donello JE, Trono D, ...

  9. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...Repair is Used to Edit Genes Read How Non-Homologous End Joining is Used to Delete Genes Additional Resources... CRISPR gene therapy, it’s important to realize that DMD is a simpler case than other genetic diseases...there is still no good treatment. The dystrophin gene is very large (79 exons), but much of the sequence...Dystrophin’s size makes it difficult to deliver via gene therapy, so researchers have set their sights on... cells expressed functional dystrophin. Editing genes in vivo is of course much more difficult than in...cells, where precision editing of the dystrophin gene would take place, with minimal risk of off-target...clinically with CRISPR. Many obstacles remain for CRISPR gene editing, but given the speed at which the technology...

  10. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...a diverse set of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based...interactions. Plasmids A diverse set of wild-type MDH genes from several species and mutant clones from watermelon..., or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs ...

  11. Synthetic Biology - Metabolism

    Type
    Collection
    ...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication Back ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  12. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...through this challenging sequence. Transgene The transgene is the gene that will be delivered by the AAV..., or your gene of interest. Promoter The promoter drives the expression of the AAV transgene. On a plasmid...plasmid which contains the structural and packaging genes, the adenoviral helper plasmid which contains the...plasmid map, promoters are upstream or 5’ of the gene they control. Promoters can be organism or tissue...for the successful and specific expression of your gene of interest. References Haery L, Deverman BE, ...Terminal Repeats of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089...control transgene expression. Lox sites are directional 34 bp sequences that can flank the transgene and ...

  13. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...repression of your target gene, activation and repression of different genes in a single cell, and localization...replication and transcription as well as reporter genes. To learn how you can use minigenomes in your research... them in action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated viruses (... (AAVs) are commonly used to deliver a genes of interest to a variety of cell types in-vivo. These are...localization of a fluorescent signal to your target gene. Read this post to find out more about how the Casilio...are widely used by the optogenetics and chemogenetics communities to activate neurons and neuronal pathways...guest writers. Your contributions make Addgene and the Addgene Blog useful resources for the biological...

  14. RUBY-Red Siliques

    Type
    Blog Post
    ...fluorescent proteins. Selection using resistance genes usually involves killing the untransformed seedlings...individual. For example, with CRISPR-Cas9 gene editing, transformation may be identified by resistance ...continued production of new alleles or off-target gene editing. Antibiotic selection would only then kill...requires specialized equipment. Reporters for studying gene expression have similar drawbacks, often requiring...Res. (2020).   I can do this by expressing a gene that rescues the mutations, using RUBY as a selection.... et al. A reporter for noninvasively monitoring gene expression and plant transformation. Hortic Res ...presence of a transgene instead, which can’t be done if your selection method kills the transgene free individual...

  15. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...cross-cassette interactions in gene expression, and to generate multi-gene knockouts. Mammalian and fly ... to generate anything from individual transcription units (TUs) used to express your favorite gene-of-...While a generic MoClo system for any given species can be repurposed for CRISPR applications (gene insert...DNA assembly tool used to build single- and multi-gene constructs for a variety of applications. MoClo ...several additional capabilities, including conditional gene expression, multiplexed CRISPR, and chromosomal ...). Those looking to express high levels of their gene of interest in yeast should also take a look at ...elements for S. cerevisiae chromosomal integration and gene expression, the system includes a toolkit to express...

  16. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...Cre-dependent gene of interest in neurons. This ability to be paired with any Cre-controlled gene of interest...neuroscientists because they efficiently deliver genes to various brain regions. You can partially control...occasionally you may want to further control where your gene of interest is expressed.  A cell-type specific ...and what do they mean? AAV: A Versatile Tool for Gene Expression in Mammals Subscribe to Viral Vector ...! To find more viral vectors available through Addgene, be sure to check out our viral service page and...Vector Blog Posts Additional Resources on Addgene.org Check out our Viral Service Page Browse Additional ...

  17. TREAT-AD Plasmid Collection

    Type
    Collection
    ...this table based on plasmid name, gene/insert, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations...of drug targets in Alzheimer’s disease (AD) by generating validated resources for emerging targets in AD...IUSM-Purdue TREAT-AD centers will be deposited at Addgene and available to request. Plasmids Browse the table...

  18. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ... allowing for the gene on the toehold switch to be expressed. In this case, the gene encodes the LacZ𝛼...interfere with other genetic pathways making it difficult to link a phenotype to the gene being targeted via...need for mutant genetic backgrounds. The standard RNAi vector, L4440, places a target gene between two inverted..., highly efficient RNAi construct for C. elegans gene knockdown Article contributed by Alyssa Cecchetelli...extensively used in C. elegans research to study gene function. RNAi is commonly achieved by feeding E... from each promoter progresses past the specific gene producing long unspecific RNA fragments that are... most scientists use mutant animals that are genetically altered to overexpress endogenous RNAi pathways...

  19. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...stably integrate a reporter gene that contains a puromycin resistance gene followed by the target site...that targets your desired gene (whose sequence is also found in the reporter gene). A control RFP expression...donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP. This visual read ... express RFP while cells containing the reporter gene that was cleaved by Cas9 and repaired via HDR using...unedited cells. In an experiment that targeted the gene NFE2L2, the Madison lab found that H2B-GFP expressing...desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein...stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems...

  20. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...inducible yet permanent gene excision. Essentially, LoxP sites are knocked in flanking a gene of interest (or ... the gene for your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift...? Is the regulation of an exogenously introduced gene or over the native locus? Let’s explore each of...Cas9 fused to a transcriptional repressor to your gene of interest. It will inhibit transcription instead...cinch. Stably introducing the coding region of a gene (cDNA) can be done by either plasmid or virus. A...Cas9. The system is led, via a custom gRNA, to your gene of interest to temporarily increase expression. ...of action Check out Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene vector. Degron tags...
Showing: 281 - 300 of 1140 results