We narrowed to 365 results for: Pol;

1. Viral Vectors 101: Producing Your rAAV

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   ...rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution...

2. Plasmids 101: Degron Tags

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   ...endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein...

3. Hot Plasmids and Viral Preps - May 2021

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   ...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...

4. Tips for a 1st time AAV user (by a Rookie AAV user)

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   ... (cap) genes. AAV replication uses the host’s polymerase, but requires Rep proteins to process a double-stranded...

5. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   ...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...

6. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   ...Chemical transfection   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines...

7. Light Sheet Fluorescence Microscopy

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   ...Praparaten. Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson, M.W. Klymkowsky. (1989) Development, 105.61-74...

8. An Introduction to Adenovirus

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   ...initiating the whole process – such as the viral DNA polymerase – it makes sense that vectorization strategies...

9. Uncovering Ancestral Bias in CRISPR Research

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   ...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...

10. Droplet Digital PCR for AAV Quantitation

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    ...resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts related ...

11. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts...

12. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ... these fluorophores are often bulky and contain polar groups (Figure 2b), which can preclude their use...

13. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    ...has four layers of location which can all be extrapolated from the 2D barcode and LIMS: Barcoded Tube...

14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    ...interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ...

15. Deciphering the Mysteries of Behavior with Viral Vectors

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    ... psychiatric diseases such as schizophrenia or bipolar disorder. In both diseases, animals have reduced...

16. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ..., including investigations into environmental pollutants and health, embryo growth, brain function, and...

17. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    ...Wieschaus. "Mutations affecting segment number and polarity in Drosophila." Nature287.5785 (1980): 795-801...

18. Lab to Office Culture Shock

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    ...of the causes of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list...

19. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post

    ...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates...

20. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post

    ...engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence...