We narrowed to 569 results for: SHA
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TypeBlog Post...navigate career changes and overcome barriers by sharing broader perspectives and holding one another accountable...perspective. Sometimes it is helpful to find peers that share a similar direction or problem. For example, a group...
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Antibodies 101: Fab Fragments
TypeBlog Post...Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot Abi? ...chain. When assembled, the four chains make a Y-shaped protein that can be divided into the Fragment Crystallizable... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...but they are laborious to generate and costly to share. What is a RANbody? A RANbody is a fusion between.... Staining with multiple HRP-RANbodies is indistinguishable since they produce the same signal, so only... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post... pathological active regions, microenvironments shape a tumor’s evasiveness, evolution, and response to...always interacting with its surroundings and being shaped by them. Another set of highly influential microenvironment... -
Antibodies 101: Affinity Reagents
TypeBlog Post...either simultaneous or independent of the other (Shahied, 2004). They are often used for clinical applications...antib8020028. PMID: 31544834; PMCID: PMC6640713. Shahied, L., et. al. (2004). Bispecific Minibodies Targeting... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...2015; Koike-Yusa et al., 2014; Ma et al., 2015; Shalem et al., 2014; Wang et al., 2014), knock down (Gilbert... 26190106. PubMed Central PMCID: PMC4559080. 8. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout... -
Addgene's Tips for Plasmid Quality Control
TypeBlog Post...One of the best things about sharing plasmids through Addgene is that we provide an added level of confidence...Serial Cloner, and Snapgene), and save these to a shared computer or server. This will ensure that future... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post...recipe like the ones shared by Bio-Techne, ThermoFisher, or Millipore Sigma. We’ll share the basic composition... -
How to Prepare for an Industry Interview
TypeBlog Post...stories to answer interview questions. Story telling shares many of the same elements as a STAR answer: a setting...questions can be hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...have shown that the conditions Sotos-type 2 and Marshall Smith Syndrome are due to mutations in the NFIX...diagnosis of NFIX-related syndromes (Sotos-type 2 and Marshall-Smith) and could be expanded to NFIA-, NFIB- and... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post... readers to more information if they desire it. Shapes. In this type of poster, written and graphical ...trickier to make figures fit nicely into circular shapes rather than a square or rectangular boxes. Background... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...competing mobile genetic elements. Image by Basem Al-Shayeb in the Doudna Lab. Scientists looking for more...your experiments? AAV-flex-taCasp3-TEVp from Nirao Shah's and Jim Well's lab induces cre-dependent apoptosis... -
Tips for Technical Support Calls
TypeBlog Post...help emails and phone calls, I feel compelled to share my years of accumulated wisdom so that you, the ...concern is based on data you have produced, please share the data. Maybe not all, but many bio companies ... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...Vision and Ophthalmology Research (ARVO). Hopefully sharing my experience will show you how you can get started...and benefited greatly by improving my skills and sharing with other more junior leaders what I had experienced... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post...can rely on one another to celebrate good news or share a bottle of wine after a rough day, and we have ...making the switch from academia to entrepreneurship, share your thoughts with us in the comments below. We’... -
Plasmids 101: Protein Expression
TypeBlog Post...been used for protein expression. Plant systems share many of the same features and processing requirements...loves being able to help the scientific community share plasmids. Additional Resources Thermofisher Protein... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post...Like Addgene, the Drosophila community is highly sharing-oriented, and there are many resources to help ...fly community. Drosophila reagents are generously shared among the research community. They maintain many... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...that matures quickly and the other slowly, see Verkhusha et al.), one benefit of the common FP timers is...19889833. PubMed Central PMCID: PMC2801723. 3. Verkhusha, Vladislav V., et al. "An enhanced mutant of red... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post... technology: because they necessarily alter the shared environment, they must never be used without popular...associated ecosystems. Because the environment is shared by everyone, he is deeply concerned with ensuring... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False, autoplay=False, hidden_controls... on the Addgene blog Learn more about plasmid sharing during COVID-19 Resources on Addgene.org Find...