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We narrowed to 712 results for: STO

Showing: 281 - 300 of 712 results

  1. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...college I studied development and evolution of histone genes in Strongylocentrotus purpuratus, the spiny...Discovering ways to culture, propagate, transform, store, and share all of these new organisms continues ...promiscuous and the gene that is responsible is understood. It’s possible to actually silence this gene ...

  3. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...frustrating it can be to get a “bad batch”); Customizable sensitivity and detection - since they frequently...changes after target binding, aptamers are highly customizable in terms of how they actually “report” as biosensors...a higher melting temperature than its Spinach ancestors, and has a lower magnesium dependence. Though ...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ... Commons license.  Histograms  For analysis of a single marker, a histogram showing frequency of events... “true” signal.  Figure 8: A series of histograms from Alyamani et al., 2018, showing number of...

  5. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...like the cold or flu) Overuse of antibiotics in livestock farming Lengthy courses of treatment with antibiotics...those bacteria that are partially resistant. If you stop a treatment early, you may be un-wittingly selecting...used in the clinical laboratory. What can we do to stop the emergence of antibiotic resistance?  The problem...

  6. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...a room, but still have control over focus, zoom, stop/start record, and allow me to see an image.  Remote...control some basic functions, including starting and stopping recording).  Test: It was unbelievable how well...the beating heart of Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need...

  7. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...modification with fatty acyl chains. For instance, myristoylation requires an N-terminal consensus sequence (...variants and check the functionality of the fusion (Mastop, 2018). Summary GFP is an all-round useful genetically...21619590. PubMed Central PMCID: PMC3129320.  18. Mastop, Marieke, et al. "A FRET-based biosensor for measuring...

  8. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...to capture ideas, write down certain notes or brainstorm, but we have to admit that paper-based record... and included hundreds of researchers. The full story is available here. Our questions focused on researchers...all types of data, which enables researchers to store everything in one place. From pictures to excel ...

  9. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...at the Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...integrin antibodies comes from IPI’s work with the Boston Children’s Hospital and Harvard-based lab of Tim...

  10. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... in your specific experimental context (Pillai-Kastoori et al., 2019). Tissues or cell lines other than...and references References Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory...://doi.org/10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache ...

  11. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...the recombined product, you’d microinject into blastocysts to be implanted in pseudopregnant female mice...s a guide that directs cleavage right before the stop codon!  Figure 3: How to design a C-terminal...guide would be selected, since it cleaves at the stop codon TAG. Design your repair template with ...

  12. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...2006): 703-9. PubMed PMID: 16582903. Potter, Christopher J., et al. “The Q system: a repressible binary...from this publication at Addgene. Wei, Xing, Christopher J. Potter, Liqin Luo & Kang Shen. “Controlling...s41598-022-07852-7 De Boer, Herman A., Lisa K. Comstock & Mark Vasser. “The tac promoter: a functional...

  13. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...and whose structures are just beginning to be understood. Therefore, in addition to their utility in the... strain that you can use for long-term library storage. After overnight growth, scrape up, pool and extract...during one of these steps is also a convenient stopping (ie. going home and eating and sleeping) point...

  14. Finding Your Perfect Job After University

    Type
    Blog Post
    ...adapt. By the end of my three months, I fully understood why parasitology roles in the field require experience... available at a small science non-profit doing customer service (recruitment agencies often don’t tell...I was also surprised to learn that I enjoy the customer service side of my job. Because of my background...

  15. Antibodies 101: Validation

    Type
    Blog Post
    ...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....mean it’s a bad antibody but more that it’s misunderstood and it could work great in another context! ...

  17. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...specific base sequences, but are sensitive to distortions in helix shape (22). Large purine:purine mismatches...enzymes.     References 1. Shuman, Stewart, and Christopher D. Lima. "The polynucleotide ligase and RNA capping...Jayakrishnan, Pravin A. Nair, and Stewart Shuman. "Last stop on the road to repair: structure of E. coli DNA ...

  18. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ... viruses were produced in high-titer retroviral stocks generated by the transfer of a retrovirus into ...gene (packaging plasmid), and 3) the vesicular stomatitis virus G protein (VSV-G) that improves the stability...24h after transfection. This supernatant can be stored at 4 °C or can be directly added to your target...

  19. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d ...performing quality control, and providing technical customer service. Team members are highly trained, and ...Jefferson dined alone.” There was a time in human history when an intelligent, motivated person really could...

  20. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...Antibodies can be used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry). This could...bind to and inhibit the function of a protein to stop its effect within the body, or they can be used ...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...
Showing: 281 - 300 of 712 results