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TypeBlog Post...you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed...to set-up emails to alert you when papers are published on your favorite topic. If you have any other...
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New Viral Vectors - March 2024
TypeBlog Post... serotype pAAV-hDlx-Flex-dTomato-Fishell_7 AAV2 Controls Fishell New serotype pAAV-mDlx-NLS-mRuby2... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog Post...A newly established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering.... “However, there had been no systematically established way of making an all-in-one vector for multiplex... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog Post...In the late 1980s, another researcher, Douglas Prasher, got the idea that this new green glowing protein...reported its sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog Post...labels! Reusing plastic Historically, scientists washed and reused materials such as glass pipettes or ...some of these plastics are high grade and can be washed and reused. This might sound risky in terms of ... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot of time...proteins for your imaging needs. If you need an FP refresher, see our FP blog posts. References Sattarzadeh... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog Post... of Human Life at the MOS. (If you want a fun refresher on the Central Dogma of Biology, I would stop ...cocktail hour, we feasted on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as ... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...that there’s a new CRISPR advance or technique published every week! One of the newest applications is ...fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...irreversible protein-peptide interactions. In a study published in Proceedings of the National Academy of Sciences...Proceedings of the National Academy of Sciences paper [published online on March 17] should cover it. Addgene: ... -
Build Your CRISPR Vocabulary
TypeBlog Post... to keep at your desk? We’ve got a CRISPR cheat sheet for that! Resources Further reading Wang, J. Y....Addgene's CRISPR 101 eBook Addgene's CRISPR Cheat Sheet Resources on the Addgene blog A Needle in a Base-Stack... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...stabilize DNA-protein interactions for subsequent sheering of DNA by sonification. Though it helps capture... your own lab Very recently, the Henikoff lab published a preprint paper outlining improvements to the... -
Adenovirus Guide
TypeGuide... you're new to viral vectors or simply need a refresher, we've included a glossary at the end! Browse ...remain the most popular choice due to their well-established methods, relative simplicity of production, and... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...serotype of our AAV preparations. We recently published a description of the VGS process and use cases...doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao R, Maurer A, Mevel M, ... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...video of her singing about the paper recently published by her lab and Mustafa Khammash's lab at ETH Zürich...UriAlonWeizmann, here my song about our paper recently published in @nchembio Title: Bye bye L-arabinose drive.... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can’t ...While all viruses have a genome inside a protein shell known as a capsid, some also have a lipid bilayer... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog Post... in synthetic biology and DNA synthesis. They published their results in a paper in Nature Communications... Nielsen & Christopher A. Voigt also recently published a tool (trained on Addgene plasmid datasets) to... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...sensitivities can now be applied to dozens of other published FRET-based sensors. For example, his team is now... improve upon an indicator they developed and published in 2010 for visualizing the transcription factor... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post...using the power of light. In a recent article published in Nature Methods, Boyden and his team describe...can figure out what they are necessary for. We published our first paper on optogenetic tools for neuroscience... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...do just this. In under a month’s time, we’ve published an article on BioRxiv describing a comprehensive...tracking their fulfillment manually in a shared spreadsheet and it takes time away from our research. For... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...Additionally, proper citation of these Addgene IDs in published work allows readers to quickly track down the ...Resource Identifiers (CURIEs) combined with well-established prefix resolution services such as Identifiers.org...