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  2. New Viral Vectors - March 2024

    Type
    Blog Post
    ... serotype pAAV-hDlx-Flex-dTomato-Fishell_7 AAV2 Controls Fishell New serotype pAAV-mDlx-NLS-mRuby2...

  4. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...In the late 1980s, another researcher, Douglas Prasher, got the idea that this new green glowing protein...reported its sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous...

  5. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...labels! Reusing plastic Historically, scientists washed and reused materials such as glass pipettes or ...some of these plastics are high grade and can be washed and reused. This might sound risky in terms of ...

  6. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot of time...proteins for your imaging needs. If you need an FP refresher, see our FP blog posts.     References Sattarzadeh...

  7. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ... of Human Life at the MOS. (If you want a fun refresher on the Central Dogma of Biology, I would stop ...cocktail hour, we feasted on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as ...

  8. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...that there’s a new CRISPR advance or technique published every week! One of the newest applications is ...fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~...

  11. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... to keep at your desk? We’ve got a CRISPR cheat sheet for that! Resources Further reading Wang, J. Y....Addgene's CRISPR 101 eBook  Addgene's CRISPR Cheat Sheet  Resources on the Addgene blog A Needle in a Base-Stack...

  15. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can’t ...While all viruses have a genome inside a protein shell known as a capsid, some also have a lipid bilayer...

  16. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...sensitivities can now be applied to dozens of other published FRET-based sensors. For example, his team is now... improve upon an indicator they developed and published in 2010 for visualizing the transcription factor...

  19. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...analysis script should return the same output: a spreadsheet of read counts. You can use these counts to confirm...and maintenance of good representation. Addgene wishes you luck in your future library use and screening...

  20. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...do just this.  In under a month’s time, we’ve published an article on BioRxiv describing a comprehensive...tracking their fulfillment manually in a shared spreadsheet and it takes time away from our research. For...
Showing: 281 - 300 of 595 results