...of other analysis scripts available, some associated with specific libraries, but any analysis script should...hundreds of thousands if not millions of reads, this analysis must be done in an automated manner. Addgene commonly...

...as LEDs can be engineered to deliver precise, consistent, and controllable pulses of light. Complicated... heading of two-component systems (TCSs). TCSs consist of a histidine kinase that phosphorylates a response...Leakiness was also an issue; some promoter activity persisted in the inactive state. The dynamic range, defined...

...since become a gold standard in gene expression analysis and a luciferase gene (one of many available to...photon emission is measured resulting from the catalysis of a chemical reaction requiring luciferin, ATP...light-producing reaction. Many luciferase assays require cell lysis as the most efficient means to disrupt the cell ...

...upsteam sample collection process or the downstream analysis process. And ChIP itself begins at...  Crosslinking...1000 bp. This is to ensure that your downstream analysis focuses on the DNA your protein is actively interacting...complexes. Your DNA is now ready for downstream analysis via any methods suitable for isolated and purified...

...Soon you’ll figure out what prompts you need to consistently and accurately run a protocol.   Figure...hasn't. Developing simple habits like these can consistently reduce errors as you work, and the possibilities...maybe especially) suggestions you are initially resistant to. Just like any other experimental approach,...

...monoclonal Cas9 background because you’ll have more consistent levels of Cas9 expression between experiments...pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs ... References 1. Aschauer DF, Kreuz S, Rumpel S. Analysis of transduction efficiency, tropism and axonal...

...molecular switches in signal transduction pathways and consist of a trimer of three subunits: alpha (Gα), beta...ligand-bound GPCRs of interest. The TRUPATH plasmid kit consists of 20 plasmids encoding 14 alpha, 2 beta, and ..., GPCRs were first mutated via site-directed mutagenesis to bind non natural ligands. These non natural...

...way to narrow down candidates for more specific analysis, like PCR or restriction digest. Make sure you...colonies that not only have your plasmid (antibiotic resistance), but also confirm those plasmids have your insert...plasmid-containing cells without using antibiotic resistance. These rely on cell lines susceptible to or dependent...

...Cell and Regenerative Biology is to understand the basis for cardiovascular and metabolic human diseases.... activator-like effector nuclease (TALEN) kit consisting of 834 plasmids. The researchers showed they ... or using a homologous template provided by the sister chromosome. As cells take those two ends and put...

...plasmids articles. These brief articles provide a synopsis of a plasmid or group of plasmids' functions and... used a combination of deliberate and random mutagenesis to isolate the best RFP. They targeted known ...-induced mutations in ATP1A1 can render cells resistant to the small molecule ouabain - and editing at...carefully designed to allow for easy removal of the resistance cassette via Cre recombination. Each knockout...

...bumble bee and honey bee microbiome. The toolkit consists of modular parts that are assembled with Golden...plasmids and suitability of various antibiotic resistance markers, promoters, and inducible gene expression...into how the microbiome responds to infection, dysbiosis, and antibiotic treatment. To facilitate these...

...in vivo, they have higher reproducibility and consistency in the final product. Because the constant (non-epitope... as ankyrin repeat proteins. DARPins typically consist of 3-4 repeats, have high specificity and binding...Targeting HER2/neu and CD16 Exhibit Improved Tumor Lysis When Placed in a Divalent Tumor Antigen Binding ...

...this problem, gRNAs can be chemically modified to resist degradation while still effectively serving their...using commercially available kits or ordered as synthesis modifications when purchasing your gRNA. Pro tip... mismatch discrimination and is more nuclease-resistant, resulting in less off-target events. While both...

...your barcoded gRNA library sequences via oligo synthesis using the format indicated below and pool-clone... Wong et al 2015. Alan Wong is currently an Assistant Professor at the School of Biomedical Sciences...Timothy K. Lu. "Enhanced killing of antibiotic-resistant bacteria enabled by massively parallel combinatorial...

...stem cells isolated from two patients with cystic fibrosis (Schwank et al). They were able to demonstrate...SCD in 2022 as well. Hereditary transthyretin amyloidosis (hATTR) hATTR is caused by a variety of single...Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. 13, 653-658 (2013)....

...fragments for bimolecular fluorescence complementation analysis under physiological conditions. Shyu YJ, Liu H... EYFP Gateway vectors with EYFP split at 175 Arabidopsis homolog of the yeast TREX-2 mRNA export complex...

...Polyclonal antibodies are produced in animals and consist of a mixture of antibodies recognizing many different...hybridomas.  First, the long-term stability, consistency between batches, and molecular definition of ...10.7554/elife.43322 Baker M (2015) Reproducibility crisis: Blame it on the antibodies. Nature 521:274–276...

..., or other. Bacterial Resistance Enter bacterial antibiotic resistance encoded in the plasmid that...Cloning Method Choose from: Gateway cloning, gene synthesis, Gibson cloning, ligation-independent cloning,...

...Performance When targeting single genes, SAM consistently shows the highest levels of gene activation ...which are relatively large and are not expressed consistently throughout cells. VPR Description VPR fuses ... dCas9 to activate transcription. This complex consists of the VP64 activator used in other CRISPR activation...

...categories: probes and editors. A probe array may consist of at least two sensor modules targeting a specific... which can be easily visualized.  The tool-kit consists of highly useful vectors compatible with Gibson...dCas9 expression and contain different antibiotic resistance markers. New CRISPR pooled library from Keith...