We narrowed to 731 results for: Sis;

...your barcoded gRNA library sequences via oligo synthesis using the format indicated below and pool-clone... Wong et al 2015. Alan Wong is currently an Assistant Professor at the School of Biomedical Sciences...Timothy K. Lu. "Enhanced killing of antibiotic-resistant bacteria enabled by massively parallel combinatorial...

...bumble bee and honey bee microbiome. The toolkit consists of modular parts that are assembled with Golden...plasmids and suitability of various antibiotic resistance markers, promoters, and inducible gene expression...into how the microbiome responds to infection, dysbiosis, and antibiotic treatment. To facilitate these...

...in vivo, they have higher reproducibility and consistency in the final product. Because the constant (non-epitope... as ankyrin repeat proteins. DARPins typically consist of 3-4 repeats, have high specificity and binding...Targeting HER2/neu and CD16 Exhibit Improved Tumor Lysis When Placed in a Divalent Tumor Antigen Binding ...

...graduate students who work as teaching or research assistants to join unions. The topic of unionization continues...of Howell Communications Philip Brenner, PhD - Assistant Professor of Sociology. University of Massachusetts...PhD - Biotechnology Communications Specialist at AsisChem Inc. and Social Media Content Specialist at Thermo...

...gene networks for the detection of antibiotic resistance and strain-specific diagnostics for the Ebola...Pardee and Alexander Green! Keith Pardee is an Assistant professor in the Leslie Dan Faculty of Pharmacy...the environment.     Alexander A. Green is an Assistant professor at the Biodesign Center for Molecular...

..., or other. Bacterial Resistance Enter bacterial antibiotic resistance encoded in the plasmid that...Cloning Method Choose from: Gateway cloning, gene synthesis, Gibson cloning, ligation-independent cloning,...

...Performance When targeting single genes, SAM consistently shows the highest levels of gene activation ...which are relatively large and are not expressed consistently throughout cells. VPR Description VPR fuses ... dCas9 to activate transcription. This complex consists of the VP64 activator used in other CRISPR activation...

...fragments for bimolecular fluorescence complementation analysis under physiological conditions. Shyu et al. Biotechniques...EYFP Gateway vectors with EYFP split at 175 Arabidopsis homolog of the yeast TREX-2 mRNA export complex...

...stem cells isolated from two patients with cystic fibrosis (Schwank et al). They were able to demonstrate...SCD in 2022 as well. Hereditary transthyretin amyloidosis (hATTR) hATTR is caused by a variety of single...Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. 13, 653-658 (2013)....

...Polyclonal antibodies are produced in animals and consist of a mixture of antibodies recognizing many different...hybridomas.  First, the long-term stability, consistency between batches, and molecular definition of ...10.7554/elife.43322 Baker M (2015) Reproducibility crisis: Blame it on the antibodies. Nature 521:274–276...

...categories: probes and editors. A probe array may consist of at least two sensor modules targeting a specific... which can be easily visualized.  The tool-kit consists of highly useful vectors compatible with Gibson...dCas9 expression and contain different antibiotic resistance markers. New CRISPR pooled library from Keith...

...silencing of self-genes. Dr. Mello presented a hypothesis that these pathways are involved in RNA based...address the growing clinical problem of antibiotic resistant bacteria. See the picture to the left for his ... vectors are engineered to replace the drug resistantance genes in the bacteria thus making the bacteria...

...living cells. Essentially this reporter construct consists of one substrate (the histone-derived peptide)... of these sites was controlled, the reporter consistently showed significantly different FRET levels between...complex or multifactorial diseases, enabling faster diagnosis and treatment and improving patients lives. References...

...able to study many cellular processes, including mitosis, mitochondrial fission/fusion, nuclear import, ...several groups have designed modular toolkits to assist with tagging genomic loci in other model systems...including mammalian cells. CRISPaint (CRISPR-assisted insertion tagging), developed by the Hornung Lab...

...cloning rings to isolate colonies of antibiotic-resistant cells; during the time required to grease, place... below) is simple: After obtaining antibiotic-resistant colonies, use a Sharpie to circle 12-48 individual...specific wells from the 48-well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have...

...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown...Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed...

...refers to the second step of this process: the synthesis of proteins from mRNA and the addition of post-translational...Overall the general strategy for protein expression consists of transfecting cells with your DNA template of...the infected host cell is eventually lysed. Cell lysis halts protein production, but there are non-lytic...

...-transformed cells yielded rates of targeted mutagenesis similar to those achieved by isogenic AdVs encoding...Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan CA, Rader DJ, Musunuru K (2014...CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep 4:...

...results you need.  Whichever method you choose, resist the temptation to over interpret your results: .... PMID: 15833050. Ma L, Chung WK. Quantitative analysis of copy number variants based on real-time LightCycler...amplification of Plasmodium falciparum multidrug resistance-1 gene (pfmdr1). J Parasitol. 2011 Oct;97(5):...

...one.   SMASh Small molecule-assisted shutoff (SMASh) functions on the basis of both a degron and a viral...Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC) was developed for ...