We narrowed to 730 results for: Son

...error rate for long sequence knock-ins by: Lucie Wilson Le Cong’s lab has developed a method using dCas9...error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error rate seen with Cas9 editors...barcodes for live cell clonal multiplexing by: Rachel Leeson Multiplexed images in live cells remains a challenge...

... variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas 12a expression cassettes when...0600-6. The Penn State Protein Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system...mammalian genome regulation and editing by: Rachel Leeson The large size of CRISPR-Cas effectors and fusion...

...Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, ...JoVE. https://doi.org/10.3791/54832  Lipman NS, Jackson LR, Trudel LJ, Weis-Garcia F (2005) Monoclonal ...

... the genome. Mendenhall and Richard Myers of HudsonAlpha (where Mendenhall is also an adjunct faculty ...the function of the protein. Myers: We’ve done comparisons of our new system versus standard ChIP-seq for... for transcription factors, but there are many reasons you might want a system like this. It’s actually...

... Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson EA, Giovannangeli C, Jasin M, Brunet E...P, Burkhalter MD, Oh S, Qaqish BF, Moore DT, Hendrickson EA, Ramsden DA (2014) The fidelity of the ligation...

...want to cover during the year. Suggest that each person bring 3 topics and write them on a board to see... and meet regularly no matter what, even if one person can’t make it. One of the biggest group mentoring...meetings. Experiment with the process. Have each person write a Development Plan. Set concrete goals with...

... on what makes them that way. Is there a senior person whose career path you are interested in following.... Finding potential mentors is one of the many reasons to always be growing your network. If you don’t...need for your current position Understand your interpersonal challenges if they know some of the people you...

...decades to generate knock-out mice, but for various reasons it is not nearly as efficient in human stem cells...genetic background and do an apples-to-apples comparison of all those mutations. If you make induced pluripotent...backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN kit to CRISPR...

... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. Susan Keown’s science...applied and I got it.” Science writing at Fred Hutchinson Cancer Research Center Work as a Science Writer...as you can to get your name on things.” Susan personally found this easier to do while still in school...

...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies... toxic protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded...killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol...

...Abrahrnsen L, Oesterlof B, Josephson S, Oestling M, Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale...

...at chromosome 19q13.4 qtr. (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety level...General viral handling safety   Containment and personal protection are key to lab safety and the same ...reduce the risk of an accident (the most common reason for lab accidents is not following safety protocols...

...to non-scientists and may make your perfectly reasonable paragraph unintelligible to a lay audience. ...HR on the judging panel. It’s important for this person to get a clear and concise explanation as to why...Writing Professional Facilitate Career Planning and Personal Development with an IDP Get Tips on Keeping Up...

... of tea, and you are more of an audio or visual person. Why don’t you record a podcast or put some effort...yourself as an educator for a short, animated TED-Ed lesson. 6) Network – Mingle with pros Where do you meet...Communication. In the US, UC Santa Cruz, MIT, UW Madison, and Boston University offer science communication...

... both NeuroMab and the Allen Brain Atlas. The comparison with two atlases enables us to see the reproducibility...literature suggest, providing an expectation and comparison on the antibody signal’s location. If a distinct... discuss the results together, explaining our reasoning to determine if the antibody is negative (poor...

....34870.  Kaas GA, Zhong C, Eason DE, Ross DL, Vachhani RV, Ming G-L, King JR, Song H, Sweatt JD (2013) TET1...linked to disease has now become much like the 1980s song “Don’t Stop Believin’”; whenever you hear it (whether...

...spike protein, called HexoPro, was developed by Jason McLellan’s lab. This version of the spike protein... Lee AG, Liu Y, Chou C-W, Byrne PO, Hjorth CK, Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, ...Seaman MS, Schmidt AG, Chen B, Neuberg D, Bajic G, Harrison SC, Wesemann DR (2021) Memory B cell repertoire...

...Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, ...piece of music. It was tough to share or remix the songs, the CDs could get scratched over time, and it was...

...PMID: 14723844. 3. Atasoy D, Aponte Y, Su HH, and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin...PubMed Central PMCID: PMC2593125. 4. Saunders A, Johnson CA, Sabatini BL. 2012. Novel recombinant adeno-...Wenbiao Chen Liquin Luo Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham Hongkui...

...adolescence passed on a heritable trait to their grandsons that decreased their likelihood of death from ... Ong-Abdullah, Meilina, et al. "Loss of Karma transposon methylation underlies the mantled somaclonal .... 3. Kaati, Gunnar, Lars O. Bygren, and Soren Edvinsson. "Cardiovascular and diabetes mortality determined...