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  1. Antibodies 101: Validation

    Type
    Blog Post
    ... is all it will bind to - particularly when you start using complex samples such as whole cell lysate ...be aware of any possible alternative translation start sites or alternative splicing that could lead to...

  2. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...breaks Prediction algorithms: A good place to start for biased validation At the moment, many software...sequences of the genome and sgRNA. This is a good starting point for most researchers as it provides a list...

  4. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...popular whole cell therapeutics because they can help start a sequence of immune reactions that aid in the fight...article, cancers differ in practically every facet starting from manifestation to their specific interactions...

  5. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...transversion editor The story of base transversion editors starts with researchers in the Keith Joung lab, who were...as AP sites. To explore this further, the team started engineering a CBE to be worse at its original ...

  6. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...specified by the Cas protein These elements were the starting point towards the development of the first cytosine...DNA before the edit is made. Using ABE7.10 as a starting point, Gaudelli evolved the base editor into 40...

  7. Technical Design of a Western Blot

    Type
    Blog Post
    ...methods and buffers to choose from. If you are starting from tissue, you’ll likely want to use a mechanical... to be read fairly soon after the reaction has started. Types of chemiluminescence enzymes The two most...

  10. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...winning submissions and deliver the prizes at the start of the New Year. Will your lab be a lucky winner...

  12. The 12 Days of CRISPR

    Type
    Blog Post
    ...'s lineup? Right! We're ready to, so let's get started!  On the 12th day of CRISPR, Addgene gave to ...

  13. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...different theme for each day. Beginning today, we’ll start with a CRISPR overview and history. Tuesday will...

  14. We’re Going Viral This Week!

    Type
    Blog Post
    ...going viral” again! #AddgeneGoesViral on Twitter Starting today, our Twitter feed will be full of viral ...

  15. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar más emocionados! Después de recibir la traducción...

  18. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...wanted to mark the occasion by trying.  When we started in 2004, we were the plasmid repository. In 2016...
Showing: 281 - 300 of 562 results