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We narrowed to 616 results for: TIL

Showing: 281 - 300 of 616 results

  5. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...Sanger sequencing was considered the gold standard until about 2008 when a new, higher-throughput sequencing-by-synthesis...relied on Sanger sequencing for QC. We occasionally still use Sanger sequencing to confirm an insert that ...

  6. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...the adenine transversion editor AYBEv3. This tool still retained considerable A → G editing activity at ...rates ranging from 4.7% to 30% at tested sites. Still, it opened a new avenue for base editor development...

  7. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...enzyme-linked immunosorbent assay (ELISA) is a versatile method used to quantify the level of target antigen...discovery, and food safety.  Part of the assay’s versatility lies in the user’s ability to modify the protocol...

  9. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...one- or two-marker plots, like in Figure 3 above, until the population of interest has been successfully...far messier than biorender plots, quadrants can still help me as a reader if I'm struggling to see the...

  10. How to Be an Excellent Trainee

    Type
    Blog Post
    ...Don’t: Wait to ask questions and/or receive help until you’ve already started a time sensitive experiment...themselves.  Be mindful that your mentor will still have their own deadlines and priorities to attend...

  12. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...transferred into the plant. In the laboratory we can utilize this natural process by replacing the genes on ...genes and speeding up research. Certainly, there is still more to transient leaf transformations, but we hope...

  13. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... however, is fight smarter, not harder. We can utilize tactics like rotating antibiotic treatments, so...antibiotic is minimized, while the infection is still effectively treated. We can prescribe antibiotics...

  14. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs into one AAV, Cas9 must...Cas9. Unfortunately, certain drawbacks limit the utility of these systems: St1Cas9 requires a very specific...

  15. Bioinformatics at Addgene

    Type
    Blog Post
    ...known as channels. Since a Nextflow process can utilize any scripting language that can be executed on ...processing of our sequencing data. However, our work still wasn’t complete. In order for our QC team to smoothly...

  16. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ... yourself for potential gRNA sites, or you can utilise several online tools that do it for you. One such... afford to wait, you can hold off on genotyping until the pups are 2-4 weeks old and use the ear tag method...

  17. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ... genome. Without a working integrase, IDLVs can still deliver their genetic payload to the cell but cannot...avoid the risk of insertional mutagenesis while still allowing for stable expression of the transgene....

  18. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...GFP-like probes by supplying a single gene to cells. Still in bacteria, which do not produce BV, an additional...fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun 10, ...

  19. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized by...facilitates the choice of the proper site, although it still is worthwhile to generate several variants and check...
Showing: 281 - 300 of 616 results