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We narrowed to 617 results for: TIL;

Showing: 281 - 300 of 617 results

  1. DNA Purification Without a Kit

    Type
    Blog Post
    ...regeneration, trace amounts of DNA (3 femtograms/ul) could still be eluted from the column, as detected by qPCR, ...break-up with your DNA purification kit, you can still reduce the number of spin columns you need to complete...

  4. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs into one AAV, Cas9 must...Cas9. Unfortunately, certain drawbacks limit the utility of these systems: St1Cas9 requires a very specific...

  5. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...transferred into the plant. In the laboratory we can utilize this natural process by replacing the genes on ...genes and speeding up research. Certainly, there is still more to transient leaf transformations, but we hope...

  6. Bioinformatics at Addgene

    Type
    Blog Post
    ...known as channels. Since a Nextflow process can utilize any scripting language that can be executed on ...processing of our sequencing data. However, our work still wasn’t complete. In order for our QC team to smoothly...

  7. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...Sanger sequencing was considered the gold standard until about 2008 when a new, higher-throughput sequencing-by-synthesis...relied on Sanger sequencing for QC. We occasionally still use Sanger sequencing to confirm an insert that ...

  8. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...the adenine transversion editor AYBEv3. This tool still retained considerable A → G editing activity at ...rates ranging from 4.7% to 30% at tested sites. Still, it opened a new avenue for base editor development...

  9. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...one- or two-marker plots, like in Figure 3 above, until the population of interest has been successfully...far messier than biorender plots, quadrants can still help me as a reader if I'm struggling to see the...

  10. How to Be an Excellent Trainee

    Type
    Blog Post
    ...Don’t: Wait to ask questions and/or receive help until you’ve already started a time sensitive experiment...themselves.  Be mindful that your mentor will still have their own deadlines and priorities to attend...

  11. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...enzyme-linked immunosorbent assay (ELISA) is a versatile method used to quantify the level of target antigen...discovery, and food safety.  Part of the assay’s versatility lies in the user’s ability to modify the protocol...

  13. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... however, is fight smarter, not harder. We can utilize tactics like rotating antibiotic treatments, so...antibiotic is minimized, while the infection is still effectively treated. We can prescribe antibiotics...

  15. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ... yourself for potential gRNA sites, or you can utilise several online tools that do it for you. One such... afford to wait, you can hold off on genotyping until the pups are 2-4 weeks old and use the ear tag method...

  16. Centrifugation

    Type
    Protocol
    ...been placed in the centrifuge before you and may still be on the equipment. Before using the centrifuge...

  20. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...GFP-like probes by supplying a single gene to cells. Still in bacteria, which do not produce BV, an additional...fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun 10, ...
Showing: 281 - 300 of 617 results