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We narrowed to 797 results for: Tec

Showing: 281 - 300 of 797 results

  1. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... (reviewed in Shcherbakova et al., 2018). For detection of protein-protein interactions, we recommend ...Multiplexing and Optogenetics across Scales. Trends Biotechnol 36, 1230-1243 (2018). Shcherbakova, D.M. et al...Sivanesan, D. & Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions...encoded calcium indicator for in vivo imaging. Nat Biotechnol 39, 368-377 (2021). Shcherbakova, D.M. Near-infrared... fluorescent protein for in vivo imaging. Nat Biotechnol 29, 757-761 (2011).   ...

  2. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef...protein-protein interactions in proximity labeling techniques such as BioID (Roux et al., 2012) and APEX (Rhee...tools, such as gene manipulation and mutagenesis techniques, are available to the scientific community.  ... as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent Advances on Physiology...

  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...RNA production can be detected by using a MS2 coat protein that specifically detects a specific RNA stem...instance, pleckstrin homology domains can be used to detect specific phosphoinositides (Varnai and Balla, 2008...Cas9 is a protein, a fusion with GFP allows for detection of genomic locations (Chen et al., 2013). GFP...fluorescent proteins (Chernov et al., 2017) may improve detection. Since longer wavelengths have better tissue penetration...

  4. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...Site directed mutagenesis is a highly versatile technique that can be used to introduce specific nucleotide...naturally occuring SNPs in a plasmid context). The technique is also highly relevant in this age of CRISPR;...plasmid digestion (blue). Digestion with enzyme “B” detects primer multimerization (purple) which is evident...~3%). Note that primer duplication will evade detection in the initial RFLP analysis simply because the...on the Addgene Blog Brush up on Your Cloning Techniques Learn More about Plasmids in Our Plasmids 101...

  5. 15 Hot Plasmids from 2017

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    Blog Post
    ...authors use PhoCl in combination with this caging technique to beautifully demonstrate light-dependent Gal4... base editors The popular CRISPR base editing technique uses dCas9/Cas9 nickase fused to a cytidine deaminase... PubMed PMID: 28585549 Kim Y, et al. Nature Biotechnology. 2017. PubMed PMID: 28191901 Listen to Our ...a fully sequenced genome, many applications in biotech, and is considered the most studied Gram-positive...vivo. Utilizing CRISPR interference (CRISPRi) technology, the arrayed knockdown library targets 289 genes...In Narayan et al., they used this now classic technique to elucidate the mechanism by which SOX2 acts ...

  6. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...understanding the brain’s function is knowing its architecture, in particular the connections between different...nervous system With the advent of viral vector technology, new methods have been developed to study neuronal...consistent which may be caused by the diversity of techniques, species, and brain regions used. Evidence for... Gradinaru Lab at the California Institute of Technology generated novel AAVs (Chan et al., 2017) that...lower toxicity, higher specificity and reduced technical variability compared to chemical tracers. rAAV2...targets neonatal neurons and adult astrocytes. Nat Biotechnol 27, 59–65. PubMed PMID: 19098898. PubMed Central...

  7. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...pathways. Trends in Biotechnology, 33(2), 92–100. https://doi.org/10.1016/j.tibtech.2014.11.007...activate the pathway than traditional pharmacology techniques allow. Multiple photoswitches can be used in ...reading Abreu, N., & Levitz, J. (2020). Optogenetic Techniques for Manipulating and Sensing G Protein-Coupled...References Abreu, N., & Levitz, J. (2020). Optogenetic Techniques for Manipulating and Sensing G Protein-Coupled...

  8. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...their hot-off-the-presses papers, the awesome new technology they’re utilizing, and the tricks they’ve used...plasmids in your own lab. Summaries of the latest technologies and breakthrough research. Insights about ..., post-doctoral fellow, lab manager, research technician, or Principal Investigator. How-to guides for...a special focus on cloning and plasmid-based technologies. Commentary about scientific sharing and the...

  9. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ... Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption of the UBMTA Chapter 4... and Diplomacy, with a focus on international technology management, from the Fletcher School at Tufts...investment banking analyst at Credit Suisse’s Technology Group in Palo Alto, CA.  Benjie Chen is a co-founder...and networks. Benjie was Addgene's Director of Technology for 7 years, and is now Chief Software Engineer...

  10. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...current forming membrane pores) transfection. These techniques are still used and efficiently transfer plasmid...not been tested for HIV.  If not using proper technique, there’s always a small chance that a researcher...transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly after transfection...for virus titration: the p24 ELISA dosing method detects the expression of viral capsid antigen p24, the...Therapy, Dr. Ke Xu (Ed.), ISBN: 978-953-307-539-6, InTech. 4. Walter, W., and U. Stein. "Viral vectors for...

  11. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... itself was relatively easy. Figure 4: Detection of edited BRAF genomic loci in Cas9-expressing...dsDNA) at basepair mismatches. To use this tool to detect a BRAF gene edit, simply PCR-amplify the BRAF locus...be in the lab. Based on the power of available techniques and how quick and easy it is to share information...Sullender M, Ebert BL, Xavier RJ, Root DE. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. 10.1038/nbt...Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PubMed PMID...

  12. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

    Type
    Blog Post
    ... modular, standardized, disciplined, and open techniques. Chapter 4: Research projects underway in the...Synthetic Biology. Wide adoption of synthetic biology techniques in biology research as can already be seen with...University of Cambridge before spending time in biotech screening direct interactions between drugs and...investigates bacteria and extremophiles for biotechnology and has been directly involved in supervising...

  13. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...research.     Improved nucleic acid sequence detection Feng Zhang’s lab recently developed a platform...high-sensitivity enzymatic reporter unlocking) to detect single molecules of RNA or DNA. Now they have developed...developed this further to provide multiplexed detection, higher sensitivity, and more. Read the paper...and cleaves ssDNA which they have adapted for detecting SNPs. Learn more from this Addgene blog post...

  14. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ...backgrounds! Show off your favorite reagent, scientific technique, or plasmid repository at your next meeting.   ...blog topic to learn more about FRET and other techniques using FPs! Otherwise, check out our plasmid and... browse plasmids by field, organism, research technique, and more. Download the green DNA background. ...those backgrounds representative of your favorite technique? Our lab-themed background is a great fit for ...

  15. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...depositors who quickly shared their reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein...reagent sharing even broader and began working with tech transfer offices to make reagents available to scientists...extracting RNA without a kit. 3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas by Shravanti Suresh...University detailed five methods for using CRISPR to detect SARS-CoV-2, including many with plasmids deposited...

  16. Typing CRISPR Systems

    Type
    Blog Post
    ...classification system for the array of CRISPR technologies. Learning the general principles of this classification...systems. Type III systems are unique, as they can technically target both RNA and DNA, though it is largely...the first taught when introducing CRISPR as a technology. There are three subtypes, A–C, with the beloved...Y. (2018). Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing. Cell ...Yoshimi, K., & Mashimo, T. (2022b). Genome editing technology and applications with the type I CRISPR system...

  17. Editor's Choice, September 2016

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    Blog Post
    ...this post even more useful to your colleagues. Technique: Probe Phage Genomes for Host Binding Proteins...editor’s choice posts this month. The first, "Technique: Probe Phage Genomes for Host Binding Proteins...from the University of Alberta and describes a technique you can use to find phage proteins that bind to...2) The post describes an ingenious but simple technique to screen for proteins that could be useful for...

  18. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...are swarming to see what can be done with this technology to help patients. Dr. Stoffels and others in ...months compared to a year or greater with older technologies. Addgene is proud to be helping get CRISPR reagents..., the panelists agreed that, for example, the technology is already available today to cure sickle cell...really sophisticated”.     New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History...

  19. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    ...It’s clear that CRISPR-Cas9 technology has really changed the game for anyone looking to quickly and ...question. Additionally, a concurrent Nature Biotechnology article describes the development of a mouse...accessible before are now accessible using this technology.” GeCKO library for genomic screens Using lentivirus... Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs...

  20. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...purification tools (and can have secondary functions as detection aids.) When a single protein is expressed, with...from solution. MBP antibodies are available for detection and/or purification of MBP-tagged proteins. The...alternative to amylose resin and also provides a visual detection mechanism via applications such as western blot...of interest, especially if downstream antibody detection is desired… and yes, we have an antibody for that...
Showing: 281 - 300 of 797 results