...CTZ) or chemical variations thereof (hCTZ), or furimazine (Fz) or chemical variations thereof (FFz, CFz...GPCRs have been shown to be involved in a wide variety of biological processes, including initiating signaling...κ-opioid G αi SALB Decrease cAMP Neuronal inhibition Vardy et al., 2015 * For a discussion of alternative DREADD...respond specifically to the anti-smoking drug varenicline. This PSAM carries mutations at L131G, Q139L,...and is termed PSAM4 (or PSAM 4 ). Additional varenicline analogs, termed ultrapotent PSEMs (or uPSEMs)...Effect Outcome (in neurons) Reference PSAM4 Gly Varenicline, uPSEM 792, uPSEM 817 Cl - influx Neuronal inhibition...inhibition Magnus et al., 2019 PSAM4 5HT3 Varenicline, uPSEM 792, uPSEM 817 Na + , K + influx Neuronal...

... different proteins and small molecules from a variety of sample types (Engvall 1971). Scientists value... the concentration of antigen in the sample. A variety of chromogenic, chemifluorescent, and chemiluminescent...direct ELISA is the quickest and simplest of all varieties. In this method, users immobilize the target antigen...antibody.  While the precise setup of the method can vary, typically users first coat the multiwell plate ...

...efficiency can vary across cell types and generally requires optimization of many variables, such as seeding...transduction efficiency. Transduction efficiency can vary with titer, cell type, and other factors, so make...libraries. Protocols from the Broad Institute- A variety of protocols and guides regarding pooled libraries...

...co-founded by Kaspar, acquired by Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies...2013). Mutations that affect this interaction to varying degrees determine the clinical severity of different...et al., 2019). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu,...

... et al., 2016). For TREE they engineered a BFP variant that undergoes conversion to GFP after being targeted...changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission spectra. Thus...the test by infecting HEK-293 cells with the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for...the Wang and Brafman lab uses an engineered BFP variant that converts to GFP after a base editing event...

..., scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop simple, portable...targets in one reaction, Cas enzymes, such as variations of Cas13 and Cas12a, are combined with different...CRISPR Trans Reporter Cas12a (Cpf1) is a CRISPR Cas variant that can also cleave dsDNA similarly to Cas9 (Zetsche...Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12...

...however, that is generally not true. Because of the variations in fluorescent signals, and how the machine reads...cell, instead of fluorescent markers. There are various types of SSC and FSC that can be used for this ...SSC, as briefly alluded to above. There’s many variations in how one can gate, select, and analyze data...Jhunjhunwala, S., Aresta-Dasilva, S., Tang, K., Alvarez, D., Webber, M., Tang, B., Lavin, D., Veiseh, O...

...biology for over three decades. Widely used across various research applications, they have become indispensable...manipulating cells and organisms. They can be used for a variety of research purposes, like creating stable cell...Leavitt et al., 1996), and since then, numerous variants have been developed with different mechanisms ...PMC2496964 Leavitt, A. D., Robles, G., Alesandro, N., & Varmus, H. E. (1996). Human immunodeficiency virus type...

...single nucleotide variations. Also, FnCas9 cleaves in a range of temperatures varying between 10-50°C. ...similar to the SHERLOCK diagnostic test, this test varies in the sequence of steps and the Cas protein used...Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12...

...Gram-positive bacteria (Kellenberger et al., 2013). Other variants of Spinach include Baby Spinach, a miniaturized...fluorescent aptamers Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described...short non-coding RNAs (Autour et al., 2018). Three variants of Mango, coined Mango II, III, and IV, were then...high binding affinity to TO1-biotin. Two of these variants exhibit increased brightness compared to Mango...

... other people for a variety of projects. I was therefore forced to learn a variety of ways to manage my...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out...of my favorite podcasts. JUST GET STARTED - One Harvard Business Review article on procrastination recommends...

...state of research became more clear throughout the various sessions of the symposium. Structural problems ...and interact more with people who have taken a variety of career paths and should be able to take steps...we try to do our part in boosting awareness of various career opportunities through our career, networking... in Publishing Panel brought in editors from a variety of publications as well as young PIs and all gave...

...Presentation Once you’ve captured your images, various software programs will allow you to align the images...controls! The necessary control experiments will vary depending on your specific experimental design. ...possibilities, and there are always new and improved variations you could try. We hope this post helped you start... H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M., & Weissgerber...

... in which the plasmid was observed to exist in various multimeric forms (e.g. concatemers, dimers, trimers...detection and can provide data on the quantity of various topologies and multimers present within a plasmid...bacterial population as a mixture of monomers and various forms of multimers (Summers & Sharratt, 1984; Summers...Berg CM, Liu L, Coon M, Strausbaugh LD, Gray P, Vartak NB, Brown M, Talbot D, Berg DE. pBR322-derived ...

...different sectors. Luckily, I have worked for a variety of different organizations, so I tend to provide...commercial company; the pay was good and there was a variety of work. The work was very scheduled and it was...solve experiments over the phone or explain how various plasmids work. Most people who call or email me...your own work schedule.  I really love my highly varied role, it has allowed me to travel and to share ...

... 101: Validating Your Genome Edit, there are a variety of options for CRISPR genotyping. The most common... sensitivity Sanger Sequencing Provided 20% (variable) Sequence-level data Low-throughput, inappropriate... Targeted Amplicon Sequencing Provided 0.01% (variable) Sequence-level data, extremely sensitive Do not...sequencing: a comparison of methodologies for KRAS variant detection." Biotechniques 53.3 (2012): 182-188....

...contributed by Doug Richardson, Director of the Harvard Center for Biological Imaging and a Lecturer on...on Molecular and Cellular Biology at Harvard University. No matter whether you are a sports photographer...Richardson! Doug Richardson is the Director of the Harvard Center for Biological Imaging and a Lecturer on...on Molecular and Cellular Biology at Harvard University.     Additional Resources Online Microscopy Primer...

...Mani and Amy Gilson from Science in the News at Harvard University. Effective science communication is ...Amy Gilson. Vini Mani is a graduate student at Harvard University, former Co-director of Science in the...     Amy Gilson recently received her PhD from Harvard University, is a former Co-director of Science ...

...–10 mg *Can vary from kit to kit, check the manufacturer’s documentation **Varies by plasmid copy ...control information, but the level of detail can vary. If you’re new to using purification kits, or if...3 below contains details regarding kits from a variety of manufacturers. It also includes a rough comparison...

...constructs improved editing in various cell types, although efficacy varied among target sequences. The ...they expanded CGBE target scope by creating BE variants with other Cas9s that recognize different PAMs...capable of predicting editing outcomes of their various CGBEs at different target sequences and in different...