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Showing: 281 - 300 of 1091 results
  1. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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    Blog Post
    ...academia and industry. As a result over 90% of Addgene’s COVID-19 resources are now available to scientists...these groups were willing and able to listen to Addgene’s new approach of working with the industry-based...in the background became a normal occurrence as Addgene and our university partners quickly assessed how...allowed them to synthesize new plasmids quickly and Addgene’s quality control and archiving system allowed us...COVID-19 plasmid collection Beyond COVID-19: Addgene’s plasmid collections available to industry scientists... this alone. If you have deposited plasmids at Addgene and would like to make them available to industry... us know below! Additional resources on the Addgene blog Browse blog posts related to COVID-19 Learn...
  2. New Viral Vectors - Fall 2024

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    Blog Post
    ...preps available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...the full viral services collection or check out Addgene's Data Hub to see viral vector reports from other... the link below to keep up with the latest in Addgene's viral vector services and resources!   This blog...
  3. Build Your CRISPR Vocabulary

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    Blog Post
    ... resources on addgene.org  Addgene's CRISPR Guide Addgene's CRISPR 101 eBook  Addgene's CRISPR Cheat Sheet...looser PAM requirements, which are available from Addgene!   RNA crRNA: “CRISPR RNAs” are transcribed from...CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable guide sequence...Conclusion Ready for some more details? Check out the Addgene CRISPR guide or our CRISPR 101 ebook. We also ... Sheet  Resources on the Addgene blog A Needle in a Base-Stack: Cas9 Structural Biology...
  4. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post
    ...terms in an Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...Additional resources on the Addgene blog Learn more about other features on Addgene's website ... better way to search First, check out the new Addgene homepage. You may have noticed the search field...indicate that their plasmids are available from Addgene in the Methods & Materials or elsewhere in their...from the James Trimmer Lab published in eLife. Addgene is currently distributing 94 plasmids expressing...someone has already created and deposited it with Addgene. Combining search terms with filters lets you drill...to apply a wide range of filters isn’t new to Addgene’s plasmid search. The new design of the plasmid ...
  5. Call for Guest Bloggers

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    Blog Post
    ... Sign up to write for the Addgene blog! To sign up to write for the Addgene blog simply email us. We can...write for us! Click Here to Contribute to the Addgene Blog Possible guest topics Some topics you might...Exposure - With over 100,000 views each month, the Addgene Blog is a great way to get your name out there!...include on CVs and Resumes. An Expanded Network - Addgene will promote your blog on a variety of social media...
  6. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post
    ...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog Cas9 Activators: A Practical...specific genomic loci is possible using CRISPR, and Addgene has a number of tools for this purpose. Epigenetics...CRISPR-based epigenetic modifiers available from Addgene. For most of these constructs, catalytically dead...epigenetic modifiers, and is available as a kit from Addgene.   Find Ready-to-Use Viral Preps for Epigenetic...nbt.3199 Find plasmids from this publication at Addgene. Klann TS, Black JB, Chellappan M, Safi A, Song...nbt.3853 Find plasmids from this publication at Addgene.  Lei Y, Zhang X, Su J, Jeong M, Gundry MC, Huang...ncomms16026 Find plasmids from this publication at Addgene. Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna...
  7. How Addgene Creates Content

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    Blog Post
    ...everything else we do! The goal of Addgene’s educational content Addgene truly believes that sharing speeds...you ever wondered how we choose topics for the Addgene blog? Or why we make certain videos? In this post...You probably won’t be surprised to learn that Addgene’s mission statement extends beyond plasmids, AAVs...topics. What better space to do that than in the Addgene blog?  Fig. 1: One of our favorite content...sure things are clear, accurate, and in line with Addgene style.        Fig. 2: Reference image (...already comes from you and the questions you ask Addgene and your research community! We work to provide...with your help.     Additional Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors...
  8. With Our New Viral Service, We're Taking CRISPR Further!

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    Blog Post
    ... are members of the Addgene Advisory Board. Additional Resources on the Addgene Blog Get the Most from...plasmids for that particular organism available at Addgene. Percentages represent the fraction of that total...available to the academic research community. At Addgene, we’ve helped distribute many of these technologies...Biologically Inspired Engineering and member of the Addgene Advisory board, on the difficulty of producing ...John Doench (Broad Institute and member of the Addgene Advisory Board) says: "When you're setting up to...Getting these quality-controlled reagents from Addgene can greatly expedite a researcher's ability to ...anytime you order ready-to-use lentivirus from Addgene, you’ll also receive transfer plasmid DNA that ...
  9. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post
    ...versions of 15 different disease-associated genes. Addgene spoke to Musunuru about how the new kit works, ...and how it compares to his CRISPR/Cas9 system. Addgene: For people unfamiliar with this new TALEN kit,...either knock out a gene or knock in a mutation. Addgene: How do you use this kit to target a particular...cells. (See the complete protocol at StemBook). Addgene: How are you now using this tool? Musunuru: We ...one needs to do disease modeling in stem cells. Addgene: Tell me about the history. How had investigators...genetically modified cells in less than a month's time. Addgene: What are the implications of this kit in terms...becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN kit to CRISPR/Cas9? ...
  10. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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    Blog Post
    ... biology tools.  Addgene Data Hub: A data repository for validation data on Addgene/CLOVER viral vectors.../ protein database search and analysis tools Addgene's analyze sequence tool: Annotates features, BLASTs...restriction enzyme cut sites Open reading frame finder  Addgene vector database: Find sequences for plasmids assembled...available sources, as well as those deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences...tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence manipulation... Rachel Leeson.  Additional resources on the Addgene blog: Learn how the CRISPR software matchmaker...time and track your experiments  Resources on Addgene.org: Read our Molecular Biology Reference Find molecular...
  11. 10 Great Guest Posts We're Thankful For

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    Blog Post
    ...guest writers. Your contributions make Addgene and the Addgene Blog useful resources for the biological...It’s nearing the end of #DepositorWeek at Addgene and we’d like to show our gratitude on the blog by ...highlighting some of the great posts written by Addgene depositors. Below you’ll find brief summaries of...depositors. Interested in writing your own post for the Addgene Blog? Fill out our guest blogger form to get started.... Note: Casilio is no longer available through Addgene.  Thanks again to all of our fantastic depositors... addgene...
  12. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    Blog Post
    ...data Do you use Addgene AAV tools and want to join the ranks of our growing group of Addgene AAV data sharing... way to celebrate open data sharing than with Addgene’s AAV Data Hub! We launched this pilot in 2019 as... an open science platform for scientists using Addgene AAV viral preps to easily find and share useful...animal strain used). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over...sharing champions? If you’ve recently used Addgene AAV tools, we invite you to contribute your data to ...data on AAV data hub! Related resources on the Addgene blog Important Considerations When Using AAVs ...Using AAV for neuronal tracing Resources on Addgene.org Read our AAV guide Browse all  AAV vectors Find...
  13. Running for Rare Disease, Running for FOP, Running for AJ

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    ...FOP until a cure is available. Addgene and AJ's Avengers This year Addgene, where my wife Lianna has made...their plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the ...years. Additional Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease Researchers...helping us fight against FOP in two ways. First, Addgene is distributing ACVR1 Plasmids from a variety of...Potentiall HIV Treatments with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease...
  14. Addgene's Top 10 Blog Posts of 2015

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    Blog Post
    ...2015 has been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array ...have started providing recorded interviews with Addgene community members. In addition we’ve experienced...them a read if you’ve missed them! Breakdown of Addgene's top blog posts from 2015 Our most popular posts...plasmids in our repository use Cas9 to cut DNA, Addgene Science Communications Intern Mary Gearing explains... need them. Happy Reading!   Resources on the Addgene Blog Download the Management for Scientists eBook...101, CRISPR 101, and Career Posts Resources at Addgene Browse Our CRISPR Resources Learn More about Viral... Addgene...
  15. Fluorescent Protein Guide: Biosensors

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    Collection
    ...Browse All More Resources You may also like... Addgene Blog: Biosensors Luciferase Plasmids Optogenetics...localize to cellular compartments or organelles. Addgene’s database includes many fluorescent biosensors ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
  16. Our New Antibody Mascot is....

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    Blog Post
    ... be in touch with you soon with some brand-new Addgene swag!   Last month, we introduced you to Blugene...Blugene and Aavery's newest friend, our Addgene antibody mascot, here to help us with our upcoming affinity...this form or head over to Twitter to vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022...that has led them to their biggest discovery yet: Addgene will soon be distributing affinity reagents. Name... four will receive some rockin’ newly designed Addgene swag. Good luck and happy naming!   Fig....Submissions are open until Feb. 3, 2022.  The Addgenies will choose up to four finalists, and the final...
  17. Addgene’s Blog Gets a New Look!

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    Blog Post
    ... also find information on using Addgene’s website, the latest Addgene news, and awards that we offer there...Say hello to the new Addgene blog! Same URL, same great articles to help you with your experiments, but...articles you want to find without even leaving the Addgene blog! Just click on the search bar at the top of...Be the first to know about new articles on the Addgene blog by subscribing to instant alerts below.   ...author pages, where you can read more about the Addgenie behind the blog post and find more of their work...interview. Get in touch by emailing us at blog@addgene.org or tell us in the comments below. We’ll track...
  18. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    Blog Post
    ...share your lab protocols online Addgene Website: http://www.addgene.org/ If you’re working with plasmids...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I ...protocols. Since we began sharing this protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning...the UK, South Korea, and beyond!   Check Out How Addgene Thinks About Developing Lab Management Software...plasmids and you decide to share them using Addgene’s repository, you can also share your associated protocols...gRNA_Cloning Vector (deposited by George Church’s lab). Addgene has also cataloged a variety of useful molecular... to learn more about sharing your plasmids via Addgene? Check out all the benefits and how you can get...
  19. The time and cost required to make a plasmid

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    Blog Post
    ...Plasmids 101 eBook Resources on Addgene.org Join the community of Addgene depositors here Check out our...intervening five years, inflation has occurred. Here at Addgene, the consumables cost per stab has increased 50%...plasmids. Use repositories Repositories such as Addgene help share materials quickly and cheaply. We save...last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means we virtually always...community engagement with and use of repositories like Addgene provides large-scale savings. How large? Using ...average of 5 hours of hands-on time per plasmid, Addgene, since its founding in 2004, has saved the scientific...not an economist! Additional Resources on the Addgene Blog 5 factors to help you choose the right cloning...
  20. New Tool for Lineage Tracing: The ClonTracer Library

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    ...Resources at Addgene Find the ClonTracer Library at Addgene Find Lentiviral Plasmids at Addgene Check out... al., 2016).  Bhang deposited the library with Addgene because she wanted to make it as easy as possible...and she is excited to deposit the library with Addgene because it is a “really great way of broadly sharing...sharing these kinds of reagents.” Here at Addgene, we’re excited to see the creative new ways you put ...and for providing this powerful resource to the Addgene community! Carrie Bhang is a research investigator...PMCID: PMC4900892. Additional Resources on the Addgene Blog Learn about the Sabatini and Wood Drug Screening...
Showing: 281 - 300 of 1091 results