We narrowed to 599 results for: arl
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  TypeBlog Post... Janelia, are now available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into...
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  pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCsTypeBlog Post...reprogramming from 0.001% to nearly 0.1% [7]. In terms of hiPSC colonies, nearly 16 hiPSC colonies can now...
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  Career Insights: Technical Support SpecialistTypeBlog Post... your technical knowledge and judgement. For particularly tricky cases, team discussions can often lead...doing a lot of science communication, and is particularly excited to hear about novel developments in ...
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  Binning Singletons: Tackling Conference Networking When You Don’t Know AnyoneTypeBlog Post...conferences should strive to provide support to early career scientists, students, and those from historically...Juliet Johnston, Kat Milligan-Myhre, Kim Dill-McFarland, Lisa Gorski, Mark O. Martin, Maureen Berg, Michael...
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  Using Video to Share Your Science: We Share ScienceTypeBlog Post...article is an interactive chart where you can see the yearly interdisciplinarity index of research for single...and case study." Journal of Librarianship and Scholarly Communication 2.2 (2014): 2. Additional Resources...
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  Changing Labor Laws Bring Increased Postdoc WagesTypeBlog Post...major effect on scientists within academia, most particularly postdocs working in the U.S., whose current ...thing. Reliance on a cheap trainee workforce has clearly not been a sustainable approach to biomedical research...
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  The Challenges of Cell CultureTypeBlog Post... is still a problem today. It is now known that nearly 500 cell lines suffer from contamination with HeLa...out on low passage cells and this means you can regularly discard the cells you are working with because...
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  Getting the Most from Your Lentiviral TransductionTypeBlog Post...American Type Culture Collection and freeze as many early passage vials as possible. One problem that often...can make this assumption incorrect. One of the earliest steps in lentiviral transduction is the interaction...
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  Important Considerations in Optogenetics Behavioral ExperimentsTypeBlog Post... determine this is to examine expression of the early response gene c-fos. For example, using Chr2 stimulation...and a lot of room for error, optogenetics is not nearly as difficult as it seems. Like every other advanced...
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  Plasmids 101: The Promoter Region – Let's Go!TypeBlog Post...between cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...
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  Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell BehaviorTypeBlog Post...animals only permits visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila...PMID: 21205846. PubMed Central PMCID: PMC3082469. Tarlow, Branden D., Milton J. Finegold, and Markus Grompe...
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  Five Popular Model Organisms, Part 2TypeBlog Post...conducted a large scale sequencing and concluded that nearly all disease-linked human genes have counterparts...the most popular model amphibians in science with early research providing insight into some of the most...
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  SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/CasTypeBlog Post...distancing. With countries like South Korea, one of the earlier epicenters of the outbreak ultimately flattening... is a one-pot, one-temperature reaction, and is nearly single-molecule sensitive. In the case of HIV-1...
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  Filming Science Videos in the Age of Social DistancingTypeBlog Post...other videos. For starters, I hadn’t anticipated nearly every webcam recording only 1280x720 resolution... to labs that need them. The lab re-opened very early in the pandemic with some incredibly strict safety...
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  Fluorescent Proteins 101: When GFP lets you downTypeBlog Post...the use of bright red fluorescent proteins (e.g. mScarlet) or infrared fluorescent proteins (Chernov et ...interfere with the function of the protein. This is clearly the case when proteins undergo post-translational...
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  Adeno-associated Viruses (AAVs) for Genome EditingTypeBlog Post...vehicles for episomal gene expression and are particularly useful for gene delivery to the nervous system...is composed of single stranded DNA, which is particularly well suited for strand invasion, a key step ...
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  3 Tips to Improve HDR Efficiency for CRISPR Editing in Human CellsTypeBlog Post... undergoing two rounds of gene-editing enables scarless introduction of an intended mutation while taking...4. Kwart, Dylan, et al. "Precise and efficient scarless genome editing in stem cells using CORRECT." Nature...
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  New Optogenetic Tools for Cytoskeleton and Membrane ControlTypeBlog Post... under CC-BY 4.0 license. Building off their earlier work on Rab11, the team used optogenetic clustering...constructs to the plasma membrane or lysosomes (the earlier article also provided CIB constructs for targeting...
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  Tips for Getting a Faculty PositionTypeBlog Post... interviews but no job offers, is a failure to clearly articulate one's talents and ideas. Some key points... matter how many times I say this, candidates regularly try to convince me that their search committee...
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  Antibodies 101: Normalization and Loading Controls for Western BlotsTypeBlog Post...BioRender.com. This kind of normalization is a nearly ubiquitous tool, but proceed with caution! Loading...chain (blue) to infer or calculate things about earlier ones. In practice, multiple sources of error (red...