We narrowed to 600 results for: arl

...What is an aptamer? Nearly 30 years ago, two independent groups, led by Jack Szostak and Larry Gold, ... Assays that require recognition of ions or particularly small molecules that do not generate an immune...

... is still a problem today. It is now known that nearly 500 cell lines suffer from contamination with HeLa...out on low passage cells and this means you can regularly discard the cells you are working with because...

...American Type Culture Collection and freeze as many early passage vials as possible. One problem that often...can make this assumption incorrect. One of the earliest steps in lentiviral transduction is the interaction...

...and I met scientists from 21 different labs who clearly also share this wonder of the organism.   It’s...songs like humans learn speech. Finches must learn early, use auditory feedback to learn, babble as baby ...

...segmentation during embryogenesis. Typically, regularly spaced denticles (bristles) outline the segments... of cookie monster mutant flies are filled with early mitotic cells as well as necrotic or dead cells ...

...the introduction of this mutation can be any site early in the coding region of the gene of interest. This...Repressing target genes using dCas9-based repressors Early experiments using dCas9 in bacteria demonstrated...

... to keep up with the latest tools. In Addgene’s early days, we would ‘spot-check’ critical regions of ...us to keep pace with our current need to analyze nearly 300 sequences each week. Our internal software ...

... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, ...

...CRISPR Cas variant that can also cleave dsDNA similarly to Cas9 (Zetsche et al., 2015 ). Cas12a however...DETECTR. How does DETECTR work? DETECTR works similarly to SHERLOCK. Cas12a is targeted to a specific ...

...conferences should strive to provide support to early career scientists, students, and those from historically...Juliet Johnston, Kat Milligan-Myhre, Kim Dill-McFarland, Lisa Gorski, Mark O. Martin, Maureen Berg, Michael...

... determine this is to examine expression of the early response gene c-fos. For example, using Chr2 stimulation...and a lot of room for error, optogenetics is not nearly as difficult as it seems. Like every other advanced...

...between cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...

...BioRender.com. This kind of normalization is a nearly ubiquitous tool, but proceed with caution! Loading...chain (blue) to infer or calculate things about earlier ones. In practice, multiple sources of error (red...

...while requiring a lower concentration of sample — nearly ten times less protein. While the addition of LEAs...Methyltransferase (CHARM), with several advantages over the earlier system.  Rather than overexpressing an exogenous...

... glycosylation patterns. Surface labeling is particularly useful for classifying and sorting cells by ...Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects ...

... under CC-BY 4.0 license.   Building off their earlier work on Rab11, the team used optogenetic clustering...constructs to the plasma membrane or lysosomes (the earlier article also provided CIB constructs for targeting...

... interviews but no job offers, is a failure to clearly articulate one's talents and ideas. Some key points... matter how many times I say this, candidates regularly try to convince me that their search committee...

...into the parental plasmid (or other). This is particularly relevant in cases where a large plasmid with...introduce a point mutation in a large construct. Particularly, if a pair of unique restriction site is located...

...using the same expression plasmid. This is a particularly important control when creating a phosphoserine-incorporation...Institute for Research in Madison, Wisconsin. She is particularly interested in the intersection of cell signaling...

...cars become more technologically advanced leaving earlier versions obsolete, the techniques we use in lab...researchers at a time when sequencing was still in the early days of development. Today, researchers use both...