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We narrowed to 581 results for: arl

Showing: 281 - 300 of 581 results

  1. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...major effect on scientists within academia, most particularly postdocs working in the U.S., whose current ...thing. Reliance on a cheap trainee workforce has clearly not been a sustainable approach to biomedical research...

  2. The Challenges of Cell Culture

    Type
    Blog Post
    ... is still a problem today. It is now known that nearly 500 cell lines suffer from contamination with HeLa...out on low passage cells and this means you can regularly discard the cells you are working with because...

  3. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...American Type Culture Collection and freeze as many early passage vials as possible. One problem that often...can make this assumption incorrect. One of the earliest steps in lentiviral transduction is the interaction...

  5. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...between cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...

  6. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...while requiring a lower concentration of sample — nearly ten times less protein. While the addition of LEAs...Methyltransferase (CHARM), with several advantages over the earlier system.  Rather than overexpressing an exogenous...

  7. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... glycosylation patterns. Surface labeling is particularly useful for classifying and sorting cells by ...Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects ...

  8. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...vehicles for episomal gene expression and are particularly useful for gene delivery to the nervous system...is composed of single stranded DNA, which is particularly well suited for strand invasion, a key step ...

  9. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...how much time you can possibly handle doing a particularly annoying task and then dedicating that much ...dinner plans at 6 pm, you had better get started early enough to finish everything you need to do by 6 ...

  10. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...other videos. For starters, I hadn’t anticipated nearly every webcam recording only 1280x720 resolution... to labs that need them. The lab re-opened very early in the pandemic with some incredibly strict safety...

  12. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...conducted a large scale sequencing and concluded that nearly all disease-linked human genes have counterparts...the most popular model amphibians in science with early research providing insight into some of the most...

  13. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...distancing. With countries like South Korea, one of the earlier epicenters of the outbreak ultimately flattening... is a one-pot, one-temperature reaction, and is nearly single-molecule sensitive. In the case of HIV-1...

  15. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...the use of bright red fluorescent proteins (e.g. mScarlet) or infrared fluorescent proteins (Chernov et ...interfere with the function of the protein. This is clearly the case when proteins undergo post-translational...

  16. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... under CC-BY 4.0 license.   Building off their earlier work on Rab11, the team used optogenetic clustering...constructs to the plasma membrane or lysosomes (the earlier article also provided CIB constructs for targeting...

  17. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... interviews but no job offers, is a failure to clearly articulate one's talents and ideas. Some key points... matter how many times I say this, candidates regularly try to convince me that their search committee...

  19. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...into the parental plasmid (or other). This is particularly relevant in cases where a large plasmid with...introduce a point mutation in a large construct. Particularly, if a pair of unique restriction site is located...

  20. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...using the same expression plasmid. This is a particularly important control when creating a phosphoserine-incorporation...Institute for Research in Madison, Wisconsin. She is particularly interested in the intersection of cell signaling...
Showing: 281 - 300 of 581 results