We narrowed to 376 results for: bre

1. Gendered Innovations: Why Does Sex of the Cell Matter?

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   ... cases of cardiovascular disease, 126,000 fewer breast cancers, and 145,000 more quality-adjusted life...

2. Plasmids 101: Cre-lox

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   ...proteins to form a tetramer. A double-strand DNA break occurs within the core spacer of each loxP site ...

3. Engaging with science and society at pgEd

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   ...eugenics and research misconduct. Past ethical breaches such as the Tuskegee syphilis experiment, the ...

4. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ...stress level, origin (vendor or in-house mouse breeding colony) Environment Olfaction, temperature, ...

5. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   ...dreamed of becoming a doctor. After taking a year break in between undergraduate school and studying for...

6. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ...modifications). Ready to get started?   Figure 1: Break free from the ball and chain!   Epigenetics...

7. CRISPR in the Clinic

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   ...doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field and the greater...

8. Design Tips for Prime Editing

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   ...Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576(7785), 149–157. https...

9. Plasmids 101: The Wide World of Natural Plasmids

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   ...coli is safe to use in the lab but also causes outbreaks of food poisoning, the difference is virulence...

10. Making CRISPR Plasmids Using Fragmid

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    ... the Fragmid system, the Fragmid website, which breaks the design process down into four easy steps, provides...

11. Antibodies 101: Stripping and Reprobing Western Blots

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    ...Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks disulfide bonds HCl Adjust pH to 6.7–6.8 ...

12. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched...

13. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ....   Confirming tags and fusion proteins. We’ll break down each of these steps below. Aligning the sequence...

14. Hot Plasmids and Viral Preps - January 2021

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    ...Dual-fluorescent DSB Repair) is a double-strand break repair assay for mammalian cells. It's based on ...

15. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    ...Howell -Founder of Howell Communications Philip Brenner, PhD - Assistant Professor of Sociology. University...

16. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    ... Addgene depositor) Craig Mello. Taking his groundbreaking work in RNAi one step further, his lab is now...

17. The Materials Science of Optogenetics Experiments

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    ...location of your region of stimulation (the z-axis bregma coordinate). Because the light that is emitted ...

18. How-to: 5 Steps to a Great Panel Discussion

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    ... follow and gets things rolling. It can make or break the discussion section. And what about those audience...

19. Plasmids 101: 5 factors to help you choose the right cloning method

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    ...methods that make working with multiple inserts a breeze. Among them are some of the homology based methods...

20. Transferable Skills Guide: Managing a Team

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    ...) everything ourselves and it’s a hard habit to break. But as a manager you will be quickly overwhelmed...