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We narrowed to 335 results for: c rel

Showing: 281 - 300 of 335 results

  1. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...References and Resources References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning...into larger units or circuits with functional relevance following a set hierarchy. MoClo originally developed...technique. Though rarer, you can find MoClo that relies on Gibson and other non-Golden Gate cloning methods...libraries depend on many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed...identify the best cocktail of genes when the relationship between genotype and phenotype is best described...

  2. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...their monovalent nature. They are stable up to 80°C and can refold correctly after denaturing.  Designed...antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an alternative affinity...either re-develop or re-optimize your assay for an entirely new affinity reagent.    Fig. 1: A comparison... These are antibody mimics and are not closely related to antibodies. Instead, they are derived from a...

  4. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ... effect related to an experimental manipulation. Common inbred strains include C57BL/6, Balb/c, and 129...Mice are fed or injected with tamoxifen, which relieves natural inhibition of the Esr1 domain that blocks...

  5. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...course, many types of teams. Some teams may be relatively permanent; some teams may only need to exist ... my senior year isolating pheromones from various C. elegans mutants by myself, I would have slowly gone...

  6. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ... PubMed Central PMCID: PMC3622210. Schek N, Cooke C, Alwine JC. Definition of the upstream efficiency ... (such as a gene) and initiate the process of releasing the newly synthesized RNA from the transcription... is also known as intrinsic termination, and it relies on the formation of a GC-rich hairpin in the RNA...terminates differently. Polymerase III, for example, relies on a specific sequence and RNA secondary structure...downstream GU-rich region (shown in the figure below) releases the mRNA from the polymerase and creates a free...

  7. Antibodies 101: Conjugation

    Type
    Blog Post
    ...doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister C, Stephenson W, et al (2017) Simultaneous epitope and... involve modifying the antibody sequence or that rely on strong protein-protein interactions with other...

  8. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...genOway press release for more information. References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2 Annealing oligos C.3 Digesting...Virus may be stored at 4°C for a few days, but should be frozen at -20°C or -80°C for long-term storage...., R is a purine (A,G), and Y is a pyrimidine (C,U). G-C content should be 36-52%. Sense 3’ end should ...annealed oligo from step C.2 20 ng digested pLKO.1 TRC-cloning vector from step C.3 (If you were unable ...Store at 4°C. m. Add 5 mL of fresh media containing antibiotics to the cells and incubate at 37°C, 5% CO ...at 37°C, 5% CO 2 overnight. Day 2: b. The target cells should be approximately 80-90% confluent. c. Dilute...solution is stable at 4°C for up to one year. The powder form of polybrene is stable at 4°C for several years...

  10. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...//doi.org/10.1016/s0091-679x(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits... choose primary antibodies from more distantly related species and/or use narrowly specific secondary ... antibodies raised in different, and distantly related, species, and secondary antibodies raised in the...

  11. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...islands (regions of the DNA with a high frequency of C and G nucleotides). The conversion of cytosine to ... and methylated states. Thus, this system is a reliable tool in reporting methylation status of these ...

  12. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ... Cas9 to generate the mutant cells, respectively (C). Mutant cells are screened (D) and "hits" are identified... for which the underlying genetic cause is not entirely known. Identifying which genes are important for...

  13. Pooled Library Amplifications

    Type
    Blog Post
    ...media, reducing the incubation temperature (e.g., 30 °C), and limiting your growth time (e.g., 16-18 hours...individually count 500,000 colonies, you’ll likely rely on serial dilutions to estimate transformation efficiency... anywhere from 10 ng to 1 μg of DNA. It often correlates with the size of the library, but generally needs...

  14. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...extract light from a single, thin plane within a relatively thicker three dimensional sample (see Figure ...920-931 (2005). PubMed PMID: 16299477. 3. Eggeling C, Willig KI, Sahl SJ, Hell SW. Lens-based fluorescence...great image is contrast. The human visual system relies primarily on contrast to identify individual objects...single camera exposure. This is much faster than relying on raster scanning as is done in confocal or 2-... possible. Therefore, 2-photon microscopy which relies on deeply penetrating infra-red excitation light...scanning confocal and 2-photon microscopes which rely on building an entire image pixel by pixel are unable...

  15. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...between 1,000 nm and 1,200 nm, where there is relatively little tissue absorption, weak tissue scattering...system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging reveals an essential role...understanding of pathogen-immune cell interaction relied on in vitro studies where it was difficult to apprehend...experiments, experience has shown that some are more reliable and more efficient at tracking cells. Good two-photon...

  16. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...involve a huge group getting from point A to B to C to D, etc. 7. Don’t let a tiny [or non-existent] ... Chen co-founded Addgene in 2004. They worked tirelessly to get Addgene from a concept to an up and running...job were important parts of team buidling - they relieved some of the pressure of start up life and maintained...“work hard, play hard.” Part of the reason we sincerely like spending time together is that when it’s ...employees. The teambuilding culture helps us build relationships and thereby work better together.I realize I...encourage everyone to make an effort to build relationships with their colleagues. It can be easy to get...done everything from kayaking, to field day, to relay triathlons, to 5Ks. Many of these examples we’ve...

  17. Genetic Code Expansion

    Type
    Collection
    .... coli p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 62599 pKPY197 phenylalanyl-tRNA synthetase...C.elegans p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 63177 pMarsL274G methionyl-tRNA synthetase...coli azi-donorleucine (Anl) Bacterial ATG David Tirrell 62598 pKPY514 phenylalanyl-tRNA synthetase subunit...musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY tyrosyl-tRNA synthetase E. coli...musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 91705 pSupAR-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers...using genetic code expansion without buildup of prematurely truncated protein. Ryan Mehl 197656 BL21(DE3)... by tolC, and tyrU gene replaced by GentR) and release factor 1. Expresses archaeal MjTyrRS/tRNA pair ...

  18. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...PubMed Central PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with...Several different methods have been developed, relying on established reagents such as CRISPR/Cas9, Tol2... that coordinate development of the body plan (Farrell et al., 2018). This study also showed that in the...Nature 568.7751 (2019): 193. PubMed PMID: 30944477. Farrell, Jeffrey A., et al. "Single-cell reconstruction...

  19. Hot Plasmids February 2024

    Type
    Blog Post
    ...mChe), with co-incubation with assist peptide (AP). C) Workflow for Cas-RNP-PAGE editing of primary T cells... and minimal toxicity, including in clinically-relevant lines such as CAR-T cells. Plus, this method is...
Showing: 281 - 300 of 335 results