We narrowed to 437 results for: ena

1. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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   ... Listen to Our Interview with Previous Awardee Kwabena Duedu Resources at Addgene.org Check out our ...

2. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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   ...linked to these metadata and publications help to enable reproducibility of experiments. Additionally, proper...

3. Hot Plasmids - January 2023

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   ...only brilliantly bright, but it also withstands denaturing conditions, high temperatures, and other chaotropic...

4. Learning Technical Skills at a Lab Central Workshop

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   ...accounts. When I heard about how LabCentral aims to enable emerging biotech companies, I was excited to go...

5. Visualizing Translation at the Single Molecule Level

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   ...appear yellow, and translated RNAs appear red. To enable this color switching, TRICK requires the following...

6. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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   ...together Combining in silico and in vitro approaches enables researchers to develop safer and more effective...

7. Plasmids 101: NGS Quality Control for Pooled Libraries

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   ...proper use requires careful amplification and maintenance of good representation. Addgene wishes you luck...

8. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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   ...Image from Richardson et al., 2016.   dCas9 enables sequence replacement without cutting Our in vitro...

9. Components of CRISPR/Cas9

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   ... which will be described in more detail below, enables Cas9 to cut a specific genomic locus of many possible...

10. Synthetic Photobiology: Optogenetics for E. coli

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    ...as with other plasmid-based systems, which will enable the assembly of larger systems. The work of Schmidl...

11. Plasmids 101: Mammalian Vectors

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    ...is typically more (double) than that used for maintenance of a transfected cell line.  Keep these tips ...

12. AAV Q&A with Tim Miles

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    ...considering the need for some manner of polyA. In this scenario, you may be best served by a dual vector, split...

13. Antibodies 101: ChIP

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    ...native proteins (so antibodies validated only for denatured proteins may not be a good choice) and the amount...

14. Hot Plasmids - February 2022

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    ...enzyme activity using an enhanced Cas12a variant (enAsCas12a), minimizing off-target effects by identifying...

15. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    ...unsorted cells (Wen et al., 2018). Thus SRIRACCHA enables scientists to both quantify Cas9 activity and sgRNA...

16. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    ...researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and CP1041...

17. Targeted Mutagenesis with EvolvR

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    ...Shakked O., et al. "CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable...

18. 15 Years of Addgene: The Top 15 Plasmids

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    ...a virus can infect. The VSV-G envelope protein enables this virus to infect many different cell types ...

19. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    ...variant, Phoenix-gp, contains only Gag-Pol and enables additional flexibility in pseudotyping.   ...

20. Antibodies 101: Flow Cytometry Controls

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    ...isotype control for that particular marker. This enables you to identify any non-specific binding to know...