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  1. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...While various Cas9 mutants have been engineered to perform unique editing tasks, classic Cas9 nuclease, which...generating a monoclonal Cas9-expression line prior to performing a screen or even a single gene knockout. Top ...

  2. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...intense student engagement and joy, rather than a perfect lesson plan or outstanding test scores.  As a result...Murray. Murray’s lab uses cellular extracts to perform transcription and translation in vitro, and the...

  3. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...variable heavy (VH) chain sequences. Fusion PCR was performed to create an amplicon that  was cloned into a ...frequency within functional light chain sequences. By performing a BciVI digest of the PCR products prior to cloning...

  4. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...occurs prior to ligation as incompatible DNA ends interfere with that step. Accordingly, NHEJ has a vast toolbox...from the broken end after cleavage, which may interfere with loading of Ku and normal NHEJ activity. Other...

  6. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...determines what secondary antibody you should use when performing indirect immunoassays. The Fab regions, on the...before applying them to samples, such as when performing IHC in a sample where your secondary antibody...

  7. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...proteins reported by other research groups that performed BirA*-based affinity purification mass spec experiments...affect protein binding patterns. Hereditary Hyperferritinemia-Cataract Syndrome (HHCS), a disease characterized...

  8. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...overexpressed from a plasmid but overexpression may interfere with the system. Another method for studying protein... from them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized...

  9. What's New in CRISPR - November 2021

    Type
    Blog Post
    ... the Liu Lab found that mismatch repair (MMR) interfered with the desired resolution of prime editing ...enhanced prime editing systems improve gene editing performance at therapeutically relevant gene targets and ...

  11. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...similar rates to untreated controls. Ma et al. performed sequencing of 23 potential off-target editing ...advancement in our understanding of how CRISPR will perform in embryos but also shows that many more experiments...

  12. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...identification of desired clones.   ...Becomes a powerful cloning tool Molecular biologists first saw the...applications. While these two strains ultimately perform the same function, there is some evidence that ...

  14. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...case, we naively assume that everything will go perfectly and our software simply follows a linear progression... need to be repeated. We also now have a good interface to begin tracking analytics data for lab procedures...

  15. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...concentration, and not all providers are equipped to perform Sanger sequencing from glycerol stocks, so make...Alternatively, you can use DNA-editing software to perform alignments of multiple sequences to visually check...

  16. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...abundance of a specific clone; lineage tracing is performed via barcode sequencing. Using the ClonTracer library...the time to talk with us and for providing this powerful resource to the Addgene community! Carrie Bhang...

  18. Working with Nuclear Receptors

    Type
    Blog Post
    ...transcription factors found in metazoans. The superfamily is comprised of 48 human and 49 murine members...version of which will feature a simplified user interface, expanded numbers of curated molecules, including...

  19. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration....
Showing: 281 - 300 of 631 results