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We narrowed to 318 results for: idua

Showing: 281 - 300 of 318 results

  10. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing, for example...

  13. Plan Your Experiment

    Type
    Guide
    ...DNA, subcloning into a plasmid, and screening individual clones. PCR amplification and next-generation...

  14. CRISPR Guide

    Type
    Collection
    ...vectors. In most cases, each plasmid contains an individual gRNA targeting a single gene in a given genome...

  15. Adenovirus Guide

    Type
    Guide
    ...provided in trans . Seroprevalence Proportion of individuals within a population that present antibodies in...

  16. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...sorting (Link opens in a new window) or isolating individual cells with cloning cylinders. Workflow Timeline...medium (see below for more details) Day 1: Seed individual cells in a 96-well plate Day 2–14: Monitor cells...Some cell lines tend not to grow well in sparse/individual cultures due to lack of secreted growth factors...can be used to enhance the growth of cells in individual cultures. Other methods for enhancing single ...trypsinization and break up any cell clumps into individual cells by passing several times through a serological... microscope later when scanning the plate for individual cells, which can be difficult to find. Leave ...microscope, then you can easily scan the plate for individual cells, which should be on the same focal plane...

  18. CRISPR Library Amplification

    Type
    Protocol
    ...recombination can all impact the representation of individual plasmids in the pooled library during amplification...designed to be as general as possible but note that individual libraries may require modifications dictated ...satisfactory results for libraries up to 200,000 individual plasmids and can be scaled according to the actual...cells is sufficient for libraries up to 200,000 individual plasmids. For larger libraries scale the number...but this dramatically increases the chances of individual plasmids being lost from the pool and/or increasing...like Glycerol or DMSO solutions. Quantify the individual Maxipreps by Nanodrop, Picogreen, or Qubit. Each...

  19. CRISPR Guide

    Type
    Guide
    ...vectors. In most cases, each plasmid contains an individual gRNA targeting a single gene in a given genome...

  20. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...Carbenicillin plated over-agar Plate shows several individual colonies and effective selection. 150 µL of 2...Carbenicillin plated over-agar Plate shows less individual colonies than the 1 mg/mL plate and effective...Carbenicillin plated over-agar Plate shows several individual colonies with smaller size than the 1 mg/mL and...
Showing: 281 - 300 of 318 results