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...2014) Low Incidence of Off-Target Mutations in Individual CRISPR-Cas9 and TALEN Targeted Human Stem Cell...

... there are necessary redundancies in skills, individuals also tend to have their own specialties. This...

...nucleotide triplets, the components recognize individual nucleotides. This imparts TALENs more specificity...

...of mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is...

...it is also important to know the brightness of individual fluorophores. Table 1 describes the brightness...

...system relies primarily on contrast to identify individual objects and perceive the world around us. Without...

...knocked out at the population level, but each individual cell only has a maximum of one gene knocked out...

...provided in trans . Seroprevalence Proportion of individuals within a population that present antibodies in...

...adjust the approach to the specific end-use of an individual experiment.   Figure 3: Our recommendations...

...single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing, for example...

...fusion protein retained the PAM properties of the individual Cas9. They also mutagenized the cytidine deaminase...

...provides a more granular level of control where individual bases can be modified. Cytidine deaminase PmCDA1...

...sorting (Link opens in a new window) or isolating individual cells with cloning cylinders. Workflow Timeline...medium (see below for more details) Day 1: Seed individual cells in a 96-well plate Day 2–14: Monitor cells...Some cell lines tend not to grow well in sparse/individual cultures due to lack of secreted growth factors...can be used to enhance the growth of cells in individual cultures. Other methods for enhancing single ...trypsinization and break up any cell clumps into individual cells by passing several times through a serological... microscope later when scanning the plate for individual cells, which can be difficult to find. Leave ...microscope, then you can easily scan the plate for individual cells, which should be on the same focal plane...

...vectors. In most cases, each plasmid contains an individual gRNA targeting a single gene in a given genome...

..., the suitability of a systemic capsid for an individual application is dependent on a number of factors...

...recombination can all impact the representation of individual plasmids in the pooled library during amplification...designed to be as general as possible but note that individual libraries may require modifications dictated ...satisfactory results for libraries up to 200,000 individual plasmids and can be scaled according to the actual...cells is sufficient for libraries up to 200,000 individual plasmids. For larger libraries scale the number...but this dramatically increases the chances of individual plasmids being lost from the pool and/or increasing...like Glycerol or DMSO solutions. Quantify the individual Maxipreps by Nanodrop, Picogreen, or Qubit. Each...

...Carbenicillin plated over-agar Plate shows several individual colonies and effective selection. 150 µL of 2...Carbenicillin plated over-agar Plate shows less individual colonies than the 1 mg/mL plate and effective...Carbenicillin plated over-agar Plate shows several individual colonies with smaller size than the 1 mg/mL and...

...of resistant cells start proliferating and the individual wells become confluent, expand into larger vessels...Generation of monoclonal cell lines from expansion of individual A549 cells stably expressing Cas9. A549 cells...for 9 days. Single cells were then plated in individual wells of a 96-well plate and left undisturbed...

...plasmid DNA from it, you will want to isolate an individual clonal population (single colony) of bacteria... proceed to Recovering Plasmid DNA or use the individual colonies for other experiments....

...different sizes in the lab. Some water baths contain individual slots for heating up tubes while other water ...manual. If you’re filling up a water bath with individual slots for tubes, remember that when you add the...