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We narrowed to 809 results for: mal

Showing: 281 - 300 of 809 results

  1. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...but it is challenging to monitor because only a small number of glutamate molecules are released in each...CMV promoter drives robust gene expression in mammalian cells. Flexible tagging: Choose untagged or add...expressing tagged proteins, and stay tuned for mammalian expression and AAV plasmid sets launching later...variety of genomic edits — single-base changes, small deletions, and large insertions up to 12 kb — with...SST), vasoactive intestinal peptide (VIP), or lysosomal-associated membrane protein 5 (LAMP5) — as well...

  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...bacterial origins, Tet systems function well in mammalian cells, and TRE-containing promoters can be used...OFF. The cumate switch system functions well in mammalian cells, and can be used in the repressible manner...previous post. While originally developed for use in mammalian cells, this system has also been implemented in...Bujard H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc...-switch: a system for regulated expression in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org...

  3. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    ...allow.” Wang’s library is designed to target a smaller set of genes and it includes more guides per gene...sgRNAs, and can result in a higher titer due to the smaller size of the plasmids. The Zhang lab, on the other...possible to conduct systematic genetic screens in mammalian cells,” David Sabatini, a senior author of one...). Genome-Wide Recessive Genetic Screening in Mammalian Cells with a Lentiviral CRISPR-Guide RNA Library...

  4. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...plasmids are small circular pieces of DNA that replicate independently from the host's chromosomal DNA. They... modify, or construct a plasmid. A plasmid is a small circular piece of DNA found in bacterial cells, ...designed to introduce foreign DNA into another cell. Minimally, lab-created plasmids have an origin of replication...

  5. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...Bacterial transformation protocols require such small amounts of DNA (often on the picogram scale) that...complete a cloning experiment with a relatively small amount of DNA (in the nanograms to micrograms scale...high quantity) – Depending on the experiment, mammalian cell transfections can require quite large amounts.... Aside from the more obvious swaps like using smaller bottles or recycled cardboard packaging, there ...while others have suggested modifications to their normal kit protocols.  Yeast plasmids – If you’re extracting...

  6. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...Then at your preferred time points, sacrifice the animals and fix the samples. When you use AAVs, it typically...typically takes a few weeks to achieve optimal expression of XFPs. Any longer and the cells may start to...to show morphological abnormalities due to the excessive amount of XFPs expressed. Then, clear the samples...suppressing transcription. It is critical to express a minimal amount of tTA to achieve highest expression levels...

  7. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...cellular dynamics with light by modifying EB1. EB1 normally sits on microtubules to attract other proteins... can be used to visualize RNA localization in mammalian cells. These tools consist of cobalamin-binding...in vivo. These new “Riboglow” probes contain a smaller RNA tag, are less prone to photobleaching, and ...your own experiments, mCarmine is available in a mammalian expression vector (pcDNA3) and a bacterial expression...

  9. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...and serotype did you use and what animal subject did you use? For animal subjects it is helpful to report...experimental details (e.g. titer, injection site, animal strain used). Similar to the goodwill of Addgene... Immunohistochemistry? Did you use any relevant animal treatment conditions like drugs (Chemogenetics)...

  10. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...scicomm efforts fall under informal learning; however, many others partner with formal learning to engage students...peers can and do consume science communication.  Formal education is education that is provided through...fully and freely engage or disengage at will.  Informal education is education that is provided, typically...

  11. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...basis of transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into a cell. The...be integrated into the host genome? Expressed episomally long-term? Expressed short-term? Answering these...non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform a lipid-based...transposon system, CRISPR targeted strategy, etc.) and episomal expression will last ~2-7 days at moderate levels...

  12. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...or other affected people, man/animal vs nature, anthropomorphized animals, or even seemingly unrelated ...Summer SciComm Series! We hope you've enjoyed this small glimpse into the world of science communication ...these pieces aren't always appropriate for your formal scientific communications, they are fantastic fodder...

  13. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...Latin for 'it'), as “a generic term for any extrachromsomal hereditary determinant”. His proposal, however...1960s when scientists began to study other extrachromosomal particles, particularly Resistance or R-factors... deli in 1972. Using a deli napkin for paper, a small group of scientists including Stanley Falkow, Stanley...genes from different bacterial (and eventually mammalian) species were cloned into plasmids and new cloning...

  14. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...most of this new relationship? Consider adding formality and active goal setting to your mentoring relationships... Check out Joanne's Reddit AMA Add a little formality to mentoring Just getting together and chatting...phone, etc.)? Make agendas for your meetings, take informal minutes on what was covered and learned, especially...do after I get my PhD) and break them down into smaller, actionable steps (e.g. Meet 10 PhDs with interesting...

  15. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...Pasteur, Paris) that changed genome editing in the malaria community for the better (Nat. Biotechnol., 2014...Plasmodium falciparum (the parasite that causes malaria). I attribute their success to technical expertise...thoughtful single guide RNA (sgRNA) design, and the abnormally low GC content of the Plasmodium falciparum genome...Examples: “organism agnostic”, “model organisms”, “small genome”, “large genome”, and “organism biased scoring...Ghorbal, Mehdi, et al. "Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9...

  16. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ... to test if a type of antibodies from an Andean mammal called alpaca could neutralize the SARS-CoV-2 virus...In the example above, for example, we fostered a formal scientific collaboration between two research groups...transient partnerships where a researcher makes a small but crucial contribution to someone else’s project...find the right expert and the lack of credit for small contributions. We strive to eliminate these two ...

  17. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...to deliver Cas9 and your gRNA of interest into mammalian cells. This vector was the top requested plasmid...) V2.0 - This plasmid from the Zhang lab is a mammalian vector that expresses both SpCas9 and your gRNAs...scrambled shRNA is used as a negative control for mammalian RNAi experiments.  Find the scrambled shRNA vector...2A-GFP (PX461). pSpCas9(BB)-2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your...

  18. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...genetically engineered receptors to interact with small molecules to elicit a response in a certain cell...genetically modified receptors are activated by small molecules to either activate or inhibit neuronal...to respond to specific, pharmacologically-inert, small molecules. These new receptors were termed Designer...muscarinic receptor family and are activated by small molecule ligands such as clozapine-N-oxide (CNO)...

  19. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...same cellular location, all at once! Small molecules What type of small molecules can you monitor with biosensors...thing that you care about within your cellular or animal system. This ‘thing’ could be a neurotransmitter... those which can be applied in cell culture and animal models primarily. Fun fact: Almost all of the biosensors...

  20. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...characterized by the Oakes and Doudna Labs, which is both small and potentially less likely to elicit a strong immune...also shows promise for in vivo research due to its small size and specificity relative to Cas9. As scientists...Teng, Fei, et al. "Repurposing CRISPR-Cas12b for mammalian genome engineering." Cell discovery 4.1 (2018)...Read our CRISPR guide Find CRISPR plasmids for mammalian systems ...
Showing: 281 - 300 of 809 results