Skip to main content

We narrowed to 577 results for: multi

Showing: 281 - 300 of 577 results
  1. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... will very likely cross-react with each other. Multiple labs have tried to transfer PHP.eB's enhanced ...which may be linked to epigenetic changes, such as multiple sclerosis?  A: Basic science and preclinical researchers...
  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...diversity introduced by lambda red recombineering and Multiplex Automated Genome Engineering (MAGE) will always...progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR to target more than ...
  3. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...folder: You will often use the same data files for multiple analyses. Rather than copying these files to your...to using dates as labels, since I often work on multiple projects in a given day. For example, instead ...
  4. Antibodies 101: Conjugation

    Type
    Blog Post
    ... the visible spectrum, so they are great for multiplexing and can be used in a variety of assay types....detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...
  5. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ... kind of cellular computation, making possible multiple logic-gated steps. Beyond the lab, these systems...these tools are already deposited at Addgene in multiple formats: for use in different organisms, targeted...
  6. dTAG - You're it!

    Type
    Blog Post
    ... transcription factors with important roles in Multiple Myeloma), it is important to ensure lack of degradation...system was employed to evaluate degradation of multiple FKBP12F36V-tagged proteins using our lentiviral...
  7. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...methylation like XbaI, ClaI, etc. If you are doing multiple digestion be sure that the buffers and temperature...molar ratio. Assembly of the fragments There are multiple ways you can assemble the different parts of a...
  8. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is ...used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest...
  9. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your calcium reading with ...you need to free up crowded optical space for multiplexing, GECO sensors are available in green, blue, ...
  10. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...pre-designed sets, so identical searches can return multiple answers. You might want to take advantage of their...SplitSet finds optimal fusion sites to split it into multiple fragments. You can define the number of fragments...
  11. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...elegant system that allows for the assembly of multiple DNA fragments efficiently and accurately in a ...system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins quickly...
  12. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...which time the cells are scraped, and lysed by multiple freeze-thaw cycles. The resultant lysate is your...cytopathic effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale over the...
Showing: 281 - 300 of 577 results