We narrowed to 575 results for: multi

1. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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   ... therapy, however, because the disease affects multiple organ systems. Nevertheless, the findings are ...  Multiple Authors...

2. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   ...Also, if there is a chance you need to combine multiple transgenes in one fly, having the transgenes in...designing experiments where you want to express multiple transgenes, remember that you’ll need to integrate...

3. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   ... The theoretical brightness is calculated by multiplying EC by QY (EC*QY, sometimes normalized to the ...I Use? Choosing Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors...

4. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   ...molecular biology and scientific research. There are multiple ways foreign DNA can be introduced into cells ...References Baltrus, David A., and Karen Guillemin. "Multiple phases of competence occur during the Helicobacter...

5. Targeted Mutagenesis with EvolvR

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   ...diversity introduced by lambda red recombineering and Multiplex Automated Genome Engineering (MAGE) will always...progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR to target more than ...

6. AAV Q&A with Tim Miles

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   ... will very likely cross-react with each other. Multiple labs have tried to transfer PHP.eB's enhanced ...which may be linked to epigenetic changes, such as multiple sclerosis?  A: Basic science and preclinical researchers...

7. Viral Vectors 101: Biosensors

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   ... have been tested in vivo, in tissue preps, in multiple species, and in cell culture models. Some of them... them have even been expanded for multiplexed imaging for advanced monitoring of different factors, in...

8. Viral Vectors 101: Types of viruses

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   ...administration, such as in mouse experiments. AAV also has multiple serotypes (12 naturally occurring and more synthetic...enough for many applications, but in some cases multiple AAVs (co-infection), an alternative virus, or ...

9. Viral Vectors 101: Viruses as Biological Tools

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   ...therapies have already been approved in the US for multiple diseases, including hemophilia B and spinal muscular... Multiple Authors...

10. How to Keep a Lab Notebook for Bioinformatic Analyses

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    ...folder: You will often use the same data files for multiple analyses. Rather than copying these files to your...to using dates as labels, since I often work on multiple projects in a given day. For example, instead ...

11. dTAG - You're it!

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    ... transcription factors with important roles in Multiple Myeloma), it is important to ensure lack of degradation...system was employed to evaluate degradation of multiple FKBP12F36V-tagged proteins using our lentiviral...

12. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ...aberrantly activate the proteasome. Clift et al. tested multiple methods for TRIM-Away delivery. For small cell...FIRE-Cas9, experiments can be designed to target multiple loci at once. In addition, the approach allows...

13. Troubleshooting Your Plasmid Cloning Experiment

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    ...methylation like XbaI, ClaI, etc. If you are doing multiple digestion be sure that the buffers and temperature...molar ratio. Assembly of the fragments There are multiple ways you can assemble the different parts of a...

14. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    ...electric current of ion channels in living cells. Multiple properties of a channelrhodopsin, such as cell... as compared to direct injection. This lower multiplicity of infection (MOI) makes it challenging for ...

15. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ... Type IIS restriction sites to easily assemble multiple DNA fragments. This system has been used to construct...controlled by Cre recombination. Each cell can have multiple copies of the transgene that recombine independently...

16. Antibodies 101: Conjugation

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    ... the visible spectrum, so they are great for multiplexing and can be used in a variety of assay types....detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...

17. The time and cost required to make a plasmid

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    ...That way, you can run one PCR, one gel, etc. for multiple plasmids at once. Over time, this can save you... Multiple Authors...

18. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ...degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is ...used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest...

19. Adenoviral Vector Production and Troubleshooting

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    ...which time the cells are scraped, and lysed by multiple freeze-thaw cycles. The resultant lysate is your...cytopathic effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale over the...

20. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ...tiny DNA cutting ninjas that deliver CRISPR to multiple types of targets: primary cells, embryos, and ...structural proteins, such as the MLV protein Gag, multimerize and spontaneously assemble into particles at ...