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Addgene

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We narrowed to 567 results for: multi

Showing: 281 - 300 of 567 results

  1. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ... have been tested in vivo, in tissue preps, in multiple species, and in cell culture models. Some of them... them have even been expanded for multiplexed imaging for advanced monitoring of different factors, in...

  4. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...folder: You will often use the same data files for multiple analyses. Rather than copying these files to your...to using dates as labels, since I often work on multiple projects in a given day. For example, instead ...

  6. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is ...used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest...

  9. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...methylation like XbaI, ClaI, etc. If you are doing multiple digestion be sure that the buffers and temperature...molar ratio. Assembly of the fragments There are multiple ways you can assemble the different parts of a...

  10. dTAG - You're it!

    Type
    Blog Post
    ... transcription factors with important roles in Multiple Myeloma), it is important to ensure lack of degradation...system was employed to evaluate degradation of multiple FKBP12F36V-tagged proteins using our lentiviral...

  11. Antibodies 101: Conjugation

    Type
    Blog Post
    ... the visible spectrum, so they are great for multiplexing and can be used in a variety of assay types....detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...

  13. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...elegant system that allows for the assembly of multiple DNA fragments efficiently and accurately in a ...system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins quickly...

  15. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...genome, they are not commonly used to introduce multiple mutations. This is because as more mutations are...challenge that may arise when crossing mice with multiple mutations is if the mutations are on the same ...

  16. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...which time the cells are scraped, and lysed by multiple freeze-thaw cycles. The resultant lysate is your...cytopathic effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale over the...

  17. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your calcium reading with ...you need to free up crowded optical space for multiplexing, GECO sensors are available in green, blue, ...

  18. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...pre-designed sets, so identical searches can return multiple answers. You might want to take advantage of their...SplitSet finds optimal fusion sites to split it into multiple fragments. You can define the number of fragments...

  19. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...fast-growing cell lines (such as HEK293, which multiplies every 16 hours). So if you, for example, split... its passage number. Especially in a lab where multiple people are working on different things, this will...

  20. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...editing efficiency of prime editing, whereby a multitude of configurations can be used to introduce specific...efficiencies for diverse prime editing systems in multiple cell types. Cell, 186, 1–17. https://doi.org/10.1016...
Showing: 281 - 300 of 567 results