We narrowed to 388 results for: nans

1. CRISPR 101: Non-Homologous End Joining

   Type

   Blog Post

   ...Ruis B, Oh S, Tomkinson AE, Hendrickson EA, Giovannangeli C, Jasin M, Brunet E (2014) Chromosomal Translocations...

2. Plasmids 101: Yeast Vectors

   Type

   Blog Post

   ... mainly on the elements required for plasmid maintenance within an E. coli cell, but vectors can be widely...

3. Protocol - How to Ligate Plasmid DNA

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   Protocol

   ...Information The final step in the construction of a recombinant plasmid is connecting the insert DNA (gene or...

4. Immunocytochemistry

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   Protocol

   ...Blog Posts Antibody Guide Western Blot Protocol Recombinant Antibody Purification Protocol Introduction Immunocytochemistry...

5. Lighting Up Cell Signaling with Photoswitchable Kinases

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   Blog Post

   ...the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, suggesting PP1...

6. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   ...oligomerize in cells? This question is largely unanswered. Ideally, fluorescent proteins should act as ...

7. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

   Type

   Blog Post

   ...information about parental channelrhodopsins to create recombinant channelrhodopsin sequences. The model first removed...

8. Summer SciComm: Preprints

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   Blog Post

   ...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...

9. What's New in CRISPR - November 2021

   Type

   Blog Post

   ... the cellular MMR machinery is disrupted via a dominant negative mutation in MLH1 (PE4 = PE2+MLH1dn and...

10. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    ...against mCherry, and secondary antibodies coupled to nanogold particles, that allow a high-spatial resolution...

11. Plasmid Tools for Microbiome Studies

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    Blog Post

    ...that lacks genetic tools is the bee microbiome. Nancy Moran’s and Jeffrey Barrick’s labs at The University...

12. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    Blog Post

    ...series of plasmids to study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or...

13. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    ...about the stewardship of this in the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board...

14. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    ... concentration of all the samples by gel or by nanodrop to assemble the  fragments based on their molar...

15. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    ...Other information may also be critical to the maintenance and use of certain plasmids, such as special ...

16. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    ... mutants do not cause off-target effects at unanticipated sites and, as predicted, decrease off-target...

17. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    ...want to program a cell to identify and target a malignant cell, then you’d need the ability to turn on genes...

18. Career Coaching for Scientists: Why and Where Do I Find One?

    Type

    Blog Post

    ...sensitive to this and have coaching plans that are financially accessible. Sometimes only a few hours of coaching...

19. Working with Nuclear Receptors

    Type

    Blog Post

    ...of all organisms depends on efficient energy maintenance - through acquisition, storage, and utilization...

20. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    ...Astromoff, A., Liang, H., Anderson, K., Andre, B., Bnangham, R., Benito, R., Boeke, J.D., Bussey, H. et al...