Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 416 results for: plasmids 101

Showing: 281 - 300 of 416 results

  1. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics Guide Optogenetics Plasmids References...module, which are also available as lentiviral plasmids if you want to make a stable cell line. This design...developing embryo is accessible for injection of plasmids and other materials.    Figure 3: Optogenetic...Guide or browse our collections of optogenetics plasmids – and keep an eye out for future updates to the.... PLoS Genetics, 19(5), e1010765. https://doi.org/10.1371/journal.pgen.1010765.  Berlew, E. E., Yamada...Biology, 32(21), 4660-4674.e6. https://doi.org/10.1016/j.cub.2022.09.010.  Ridley, A. J. (2016). Rho GTPase... in Cell Biology, 36, 103–112. https://doi.org/10.1016/j.ceb.2015.08.005.  Tei, R., Bagde, S. R., Fromme...

  2. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid... Plasmid Collections Luciferase...also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled by NanoLuc® Luciferase...

  3. CRISPR Guide

    Type
    Collection
    ...editing CRISPR 101: Homology directed repair CRISPR 101: Validating your genomic edit CRISPR 101: Multiplex...Blog References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...likelihood that any cell containing the CRISPR plasmid will have all desired genomic edits carried out...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...

  5. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...lentiviral plasmids Additional resources on the Addgene Blog  How to design your CRISPR gRNA CRISPR 101: Ribonucleoprotein... Cell 81, 7, 1553-1565 (2021). https://doi.org/10.1016/j.molcel.2021.02.007 Moroz-Omori, E., Satyapertiwi...

  6. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ... about FLEx vectors Download our Plasmids 101 eBook Read our plasmids blog posts Resources on the Addgene...molecular biology reference Find Cre-lox plasmids at Addgene Browse plasmids at Addgene ...stimuli in the mesolimbic dopamine system." Neuron 101.1 (2019): 133-151. PubMed PMID: 30503173. PubMed Central...the nervous system: a clinical reality." Neuron 101.5 (2019): 839-862. PubMed PMID: 30844402. Juarez, ...

  8. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...CRISPR 101 blog series Resources on Addene.org Read Addgene's CRISPR Guide Find CRISPR plasmids for your...Expression. Cell 152:1173–1183 . https://doi.org/10.1016/j.cell.2013.02.022 Sajwan S, Mannervik M (2019)...Fluorescence Imaging. Cell 159:635–646 . https://doi.org/10.1016/j.cell.2014.09.039 Zhou H, Liu J, Zhou C, Gao N...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Blog Read Our CRISPR 101 Posts Read Our Plasmids 101 Posts Read All of Plasmid Technology Posts Resources...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase... can visualize: Cell Structure Plasmids: Cell Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61...luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids have been used extensively...find all of our Hot Plasmid articles on our website. We update the our Hot Plasmids articles every quarter...

  11. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...! More resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and When to Use Them A Control...Antibody collection Addgene's Recombinant Antibody Plasmids collection  ...

  12. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells, and ...Precision and Flexibility to CRISPR Engineering CRISPR 101: RNA Editing With Cas13  CRISPR Cheat Sheet Resources...bioRxiv 2021. 11.01.466786; doi: https://doi.org/10.1101/2021.11.01.466786 Additional Resources on the Addgene...

  14. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ... the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent...from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis...directly purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab...SapTrap Resources on Addgene.org Browse CRISPR plasmids Check out our CRISPR guide pages Find ready-to-use...

  15. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... content. Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion Don’t ...question. I like to ask about how many people use plasmids in their research and then see how many of them...

  16. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...this evolving field! Find prime editing plasmids! Find plasmids from David Liu's lab! This post was originally...the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by function...for treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first demonstration...function Browse our entire plasmid collection ... Cell, 184(22), 5635-5652.e29. https://doi.org/10.1016/j.cell.2021.09.018 Choi, J., Chen, W., Suiter, ... Cell, 186(18), 3983-4002.e26. https://doi.org/10.1016/j.cell.2023.07.039 Lin, Q., Jin, S., Zong, Y., ...

  17. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...doi.org/10.1016/j.bbamem.2013.09.005 More resources from Addgene Fluorescent protein plasmids and resources...cell and protein analysis products. You can find plasmids deposited by Promega here.    References and ...right)est fluorescent protein Fluorescent Proteins 101: A History of Fluorescent Proteins ...

  18. CRISPR Library Amplification

    Type
    Protocol
    ...differences, and plasmid recombination can all impact the representation of individual plasmids in the pooled... ). Lentiviral plasmids can recombine between their LTRs resulting in a smaller plasmid containing only...protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli cells. Protocols... protocol allows the amplification of a pooled-plasmid library in Escherichia coli cells. Pooled libraries...libraries contain tens to millions of different plasmids in a single sample. They are often used for screening...throughput multiplexed experiments. These pooled plasmids differ by only a short region of DNA: guide RNAs...results for libraries up to 200,000 individual plasmids and can be scaled according to the actual number...

  19. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-...to vary the ratio of shRNA plasmid, packaging plasmid, and envelope plasmid to obtain the ratio that gives...shRNA. psPAX2 Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing viral... also be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg....1 Protocol pLKO.1 - TRC Cloning Vector Addgene Plasmid 10878. Protocol Version 1.0. December 2006. Copyright... RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B.1 Determine...Puromycin resistance gene for selection of pLKO.1 plasmid in mammalian cells. sin 3’LTR 3’ Self-inactivating...

  20. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...validation assays are insufficient As discussed in CRISPR 101: Validating Your Genome Edit, there are a variety...Addgene.org View Our CRISPR Guide Pages Find CRISPR Plasmids for Your Experiments Get Ready-to-Use CRISPR Lentivirus...
Showing: 281 - 300 of 416 results