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  1. Working with Nuclear Receptors

    Type
    Blog Post
    ...self-propagation by reproduction. Both physiological processes are controlled by deliberate and compulsory actions...goals is to promote an understanding of how these processes are regulated at the cellular, tissue and organ...

  3. 6 Tips for Grant Writing

    Type
    Blog Post
    ...feedback. They’ve most likely been through the process themselves many times and will have useful advice...agency’s investment. 6. Failure is part of the process, keep your head up! It’s very likely that at first...

  4. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...interference (DIC) microscopy and computer imaging processing. The combination of these methods provides unique...transgene control in Caenorhabditis elegans." Proceedings of the National Academy of Sciences 115.15 (2018...

  5. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ... the nucleotide sequence during the DNA repair process. The addition of small molecules that promote homologous...Also, because non-homologous repair is a random process, zygotes edited using the same nuclease may not...

  6. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...important roles in many cellular and regulatory processes, but much of their functionality is yet to be ... of microRNA lead to its depletion and Drosha processing retardance. RNA Biol. 2014;11(10):1243-1249. ...

  7. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    .... Dry nitrocellulose membranes are brittle, so proceed with caution. Unlike PVDF, they can be returned...remains, you may want to repeat your stripping procedure, perhaps using longer incubation times, or try...

  10. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...Protein expression refers to the second step of this process: the synthesis of proteins from mRNA and the addition...Plant systems share many of the same features and processing requirements as mammalian cell expression systems...

  11. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...scientists have been able to study many cellular processes, including mitosis, mitochondrial fission/fusion...insertions can only be detected by labor-intensive processes like PCR or by evaluating visual phenotypes. Improving...

  14. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...Gene targeting takes advantage of this natural process to replace a targeted genetic locus with homologous... locus of interest. To give you an idea of the process, we’ll walk through an experiment designed to knock...

  15. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...components of Cascade for maximum efficiency of the process. Various labs have designed all-in-one Cascade ...needs to remain anchored to Cascade throughout the process. The reeling of ssDNA and unwinding of dsDNA occur...

  16. Common Injection Routes in Mice

    Type
    Blog Post
    ...delivery is also common. Due to the intubation, this procedure is considered quite invasive. Restraint: anesthesia...similar to its intratracheal counterpart, as both procedures target the lungs. The main differences are that...

  17. Transferable Skills: Negotiation

    Type
    Blog Post
    ...an offer but even as I did (successfully!), the process felt unnatural and intimidating. I felt unprepared...unhappy.   Negotiation as a creative problem-solving process Although at first glance it may appear that there...

  18. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...favorite in-house protocol. DNA yields for this procedure are typically in excess of 100 ng/ul, more than... carbohydrates that can inhibit the cell lysis process. To ensure that lysis is complete, use a reagent...

  19. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...genes or entire pathways in a particular disease process. While CRISPR is certainly not the first means ... screen using CRISPR libraries is a multi-step process (see Fig. 2). In most cases, CRISPR libraries are...

  20. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Therefore, these can be used as “timers” for cellular processes following their activation. The four main FTs ...Throwing color lights on molecular and cellular processes” Curr. Protein. Pept. Sci. 9(4):338. Chudakov ...
Showing: 281 - 300 of 618 results