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We narrowed to 630 results for: proc

Showing: 281 - 300 of 630 results

  2. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...still express ccdB and therefore would die. This procedure dramatically reduces the number of clones that...recombinant plasmid and therefore makes the cloning process much more efficient, as one does not have to thoroughly...

  5. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ... she could “get exposure to the drug discovery process.” The ClonTracer library originally came about ...that these are selected for during the treatment process. The authors went on to show that combination treatment...

  7. Working with Nuclear Receptors

    Type
    Blog Post
    ...self-propagation by reproduction. Both physiological processes are controlled by deliberate and compulsory actions...goals is to promote an understanding of how these processes are regulated at the cellular, tissue and organ...

  8. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    .... Dry nitrocellulose membranes are brittle, so proceed with caution. Unlike PVDF, they can be returned...remains, you may want to repeat your stripping procedure, perhaps using longer incubation times, or try...

  9. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...important roles in many cellular and regulatory processes, but much of their functionality is yet to be ... of microRNA lead to its depletion and Drosha processing retardance. RNA Biol. 2014;11(10):1243-1249. ...

  11. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...interference (DIC) microscopy and computer imaging processing. The combination of these methods provides unique...transgene control in Caenorhabditis elegans." Proceedings of the National Academy of Sciences 115.15 (2018...

  12. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ... the nucleotide sequence during the DNA repair process. The addition of small molecules that promote homologous...Also, because non-homologous repair is a random process, zygotes edited using the same nuclease may not...

  14. 6 Tips for Grant Writing

    Type
    Blog Post
    ...feedback. They’ve most likely been through the process themselves many times and will have useful advice...agency’s investment. 6. Failure is part of the process, keep your head up! It’s very likely that at first...

  16. Transferable Skills: Negotiation

    Type
    Blog Post
    ...an offer but even as I did (successfully!), the process felt unnatural and intimidating. I felt unprepared...unhappy.   Negotiation as a creative problem-solving process Although at first glance it may appear that there...

  17. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...components of Cascade for maximum efficiency of the process. Various labs have designed all-in-one Cascade ...needs to remain anchored to Cascade throughout the process. The reeling of ssDNA and unwinding of dsDNA occur...

  18. Common Injection Routes in Mice

    Type
    Blog Post
    ...delivery is also common. Due to the intubation, this procedure is considered quite invasive. Restraint: anesthesia...similar to its intratracheal counterpart, as both procedures target the lungs. The main differences are that...

  19. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...Gene targeting takes advantage of this natural process to replace a targeted genetic locus with homologous... locus of interest. To give you an idea of the process, we’ll walk through an experiment designed to knock...
Showing: 281 - 300 of 630 results