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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 938 results for: Kin

Showing: 151 - 160 of 938 results

  1. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...2 Labs Learn how to best protect yourself when working in BSL-1 and BSL-2 labs Watch the Video! Lab Safety...Description (Link opens in a new window) Link to Video Making LB Agar Plates Create plates to culture bacteria...antibiotic to a pre-poured plate Watch the Video! Streaking Bacteria Isolate single bacterial colonies on ...

  2. General Transfection

    Type
    Protocol
    ...After 2 months, discard the tube and thaw a new working stock. Considerations Before You Start The health...DNA. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate the mixture 15–...DMEM complete. Incubate the cells 24–48 h before checking for protein expression. Sample Data Legend: Lenti-X...

  3. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Automated cell counter 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead bath Vortex ...Millipore Sigma A6279 Before Starting Warm the DNA and working stock of PEI-MAX to room temperature before use...flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm. Pro-Tip Do not use cells...

  4. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC...get PCR amplification. We can start similarly, taking the final 18bases of the ORF, including the stop...

  5. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...Workflow Timeline Day 1: Antigen Coating Day 2: Blocking Day 3: Primary antibody incubation Day 4: Secondary...aspirate the wash buffer from the wells. Prepare the blocking buffer (1% BSA in PBS) as follows: Add 250 mg ...mix. Using a multichannel pipette, add 200 µL of blocking buffer to each well. Cover the plate with a plate...

  7. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...sites, will result in similar sized bands, thus making this simple digest less informative. This is particularly...freezer and you are not 100% sure it is what you are looking for, but you have a map and know exactly what it...

  8. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Liquid Bacterial Culture You may also like... Streaking and Isolating Bacteria on an LB Agar Plate Creating...Incubate bacterial culture at 37°C for 12-18 hr in a shaking incubator. Note: Some plasmids or strains require...

  9. Centrifugation

    Type
    Protocol
    ...appropriate for the lab space in which you are working. Even if your samples may not require specific ...centrifuge is clean and that everything appears to be working smoothly. Place your sample tubes into the rotor...

  10. Gibson Assembly Protocol

    Type
    Protocol
    ... with a two-part Gibson reaction if you're only making a small change in a plasmid (such as point mutations... fine in an assembly if you want to save time. Working on ice, combine segments in the Gibson Assembly...
Showing: 151 - 160 of 938 results