Skip to main content
Addgene
Showing: 451 - 480 of 810 results

  2. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...has been shown to work in plant, Drosophila and mammalian cells, and it may find wide use in biological ...efficiently photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible with other...

  3. Science as Art

    Type
    Blog Post
    ... is has been featured in Time magazine . Other smaller competitions are now taking place and gathering...moonlighting as artists can compete in the Nikon Small World Photomicrography Competition - a 40 year old...

  4. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ... 50 base pairs of sequence homologous to the chromosomal site of insertion. The linear PCR product is ...Based on their findings, the authors recommend optimal FP combinations for use in yeast imaging, categorized...

  5. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    .... – CRISPR can be used to generate cell line or animal models which have the precise disease genetics.... drug selectively kills the wildtype line with minimal to no toxicity in the knockout line, it is likely...

  7. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ... of interest. You can filter CLASTR results by minimal number of markers and similarity score match. This...allele search preferences to help you identify proximal lines you care about. If you have cell lines without...

  9. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...reduced by using superfolder GFP (sfGFP) with the small solubility tag GB1. In SunTag nomenclature throughout...Fluorescent Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org ...

  10. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...engineering and experimental refinement to determine the optimal localization of the Rluc8 insertion within the ...refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16 non-visual...

  12. Fostering communication in remote collaborations

    Type
    Blog Post
    ...and improves overall communication.  Maintain informal communication In between recurring meetings, you...this casual communication reflects the type of informal communication you’d likely have with members of...

  16. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ... and “exercise” consisted of taking walks in the mall. I took action, joined a gym, and started running...scientific community know, treatments that work well in animal models do not always work as efficiently in humans...

  18. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...decide if they’re alive or not. Yet these simple, small particles have quite the outsized impact — and not...of viral vectors (which many researchers will informally refer to as viruses) are named after the viruses...

  19. What the HEK?

    Type
    Blog Post
    ... is present in this line, making it genetically female. HEKs are generally cultured as an adherent monolayer...blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral Vector Production and...

  24. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...streaked bacteria have higher copy numbers – for optimal results always pick a single colony and do not ...

  27. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...genomic drift or limited material from polyclonal animal preparations (Bradbury et al., 2015). All of Addgene...were tested for specificity in knock-out lines or animals.  Addgene will continue to add more antibodies,...

  28. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...valuable for CRISPR/Cas9 stem cell editing. The small molecule NSDB and Na+ hypertonicity are key elements... Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles. ...

  29. Hot Plasmids - January 2023

    Type
    Blog Post
    ...alternatives - RNAi and Cas13. CRISPR-Csm doesn’t degrade proximal RNAs nor does it have high levels of off-target...changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from...
Showing: 451 - 480 of 810 results