We narrowed to 728 results for: STO
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TypeBlog Post...technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi is unmatched—unlike...cannot be used to investigate essential genes. Historically, gene silencing methods were thought to be afflicted...
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How to Deposit Your Plasmids with Addgene
TypeBlog Post...conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks We will need information on how to verify... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...generated the first optically active saCas9 protein. Stop light! Photoactive anti-CRISPRs halt Cas9 activity... which would add the PCK upon reaching the amber stop codon, TAG. (To learn more about site-specific incorporation... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...transport in neurons. (b) Genetic cargo can be customized to control transgene expression. Gene regulatory...Chuapoco, M. R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan, K. Y., Scherrer... -
New Antibodies in the Lab Animation!
TypeBlog Post...ELISAs, western blots, immunoprecipitation, immunohistochemistry, immunocytochemistry, and flow cytometry... -
Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!
TypeBlog Post...grade DNA! This is just the first of many milestones Addgene is poised to reach in the near future... -
2022 Depositor Week
TypeBlog Post... of Fame Thursday: 8 Great Depositor Research Stories Friday: Specialized Depositor Shout-Out We'll... -
Antibody Guide
TypeGuide...antibodies can be stored at 4 °C for short-term storage. For long-term storage they should be stored at -20 °C... Antibody Handling Storage Check the manufacturer’s instructions for exact storage requirements. As a ...tips for storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °...define key terms, summarize antibody production and storage techniques, and explain common applications. Science...complete. HRP-conjugated antibodies should not be stored in sodium azide, as it acts as an inhibitor to ...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding... -
It’s CRISPR Week at Addgene!
TypeBlog Post... today, we’ll start with a CRISPR overview and history. Tuesday will focus on generating knockouts using... -
Addgene's 20th Anniversary Party
TypeBlog Post...without recognizing our incredible depositors and requestors. Thank you all so much for an amazing twenty ... -
We’re Going Viral This Week!
TypeBlog Post...we’ll be at SfN2019. If you’re attending SfN2019, stop by booth #2163 to say hi and learn more about Addgene... -
New FLIS Plasmid (April Fools 2022)
TypeBlog Post...tiny creatures do tend to come with a rather astonishing ability to...well, annoy. Fly-related Issues... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog Post... by sharing all the incredible guest posts and stories featured in our blog in 2023. Some of these were... -
#Deckthelab Contest Winners 2017
TypeBlog Post... Although we're storing away all of our holiday decorations, our holiday spirit remains. We would like... -
Twenty Years of Sharing Science!
TypeBlog Post...scientists using materials in our repository, from our customer and science support to our efficient and easy ... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog Post...plasmids containing genes from rabies and vesicular stomatitis virus, you may have noticed it’s been easier ... -
Twelve Amazing Guest Blog Contributors!
TypeBlog Post...Contributors of 2023 8 Great Depositor Research Stories Interested in contributing to the Addgene blog?... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post...Kuhlman, B., Dantas, G., Ireton, G. C., Varani, G., Stoddard, B. L., & Baker, D. (2003). Design of a Novel ... -
DIY DNA Ladders from Penn State University
TypeBlog Post...Digest References 1. Henrici, R. C., Pecen, T. J., Johnston, J. L., & Tan, S. (2017). The pPSU Plasmids for... -
Introducing Addgene's Microbiology Pages
TypeBlog Post...Society for Microbiology Microbe meeting here in Boston next week - be sure to come by booth #851 and say... -
Deck the Lab 2022!
TypeBlog Post...safety regulations. That’s one sticky situation our customer service can’t get you out of. View... -
Bringing Renewable Energy to Addgene
TypeBlog Post... Patrick Stoney... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog Post...instructions and calculations on how to prepare a plasmid stock used to generate a standard curve, visit the AAV... -
Podcast: A Malawian Professor's Path to Biotech Research
TypeBlog Post...research 28:08-END : Kingdom's Thoughts on his time in Boston and Outro We'd like to thank Dr. Kwapata for taking... -
#DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La
TypeBlog Post...decorate your lab in pipette tip snowflakes, hang stockings filled with tube rack “gifts”, or prepare for ... -
Summer Fun at Addgene!
TypeBlog Post...The weather is finally warming up in Boston and that means summer is right around the corner. Here at... -
Modular Cloning Guide
TypeGuide...protein secretion in yeasts S. cerevisiae and P. pastoris (a.k.a. K. phaffii ). POMBOX MoClo YTK Yeast Expression...toolkit for protein expression and secretion in P. pastoris (a.k.a. K. phaffii ). GoldenPiCS Kit Yeast Expression...plasmids for advanced strain engineering in P. pastoris (a.k.a. K. phaffii ), including overexpression...cellulose-producing Komagataeibacter species. Ralstonia-GG-Kit Bacterial Expression Thomas Lahaye Plasmids...knockout and complementation constructs for use in Ralstonia solanacearum . XanthoMoClo Xanthobacter Genetic...MetClo DNA Assembly Kit Bacterial Expression Christopher A. O'Callaghan Toolkit for assembling large DNA...Bacterial Expression Huimin Zhao Plasmids to assemble custom-made RNA-binding proteins based on the Pumilio ... -
Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service
TypeBlog Post...digestions, PCR etc. and/or directly long term storage. Every preparation is quality controlled through... -
Addgene’s Blog Gets a New Look!
TypeBlog Post... us in the comments below. We’ll track down the story, get the inside scoop, and keep you up-to-date. ... -
Addgene’s Top 10 Blog Posts from 2014
TypeBlog Post...more and more scientists offering to share their stories – about their research, their job hunts, and their...