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We narrowed to 509 results for: plasmid dna

Showing: 451 - 480 of 509 results

  1. Plant Plasmids and Resources

    Type
    Collection
    ... Plasmid Collections Plant Plasmids Plant Plasmids and Resources...addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...resources: Find plasmids for use in plants. Plasmid... Background Addgene’s repository contains many plasmid tools that were specifically designed for use in...use in plants. The tables below highlight plasmids spanning a wide variety of molecular biology technologies...fluorescent proteins. Addgene’s collection includes plasmids containing promoters that drive general and species-specific...

  2. Allen Institute for Cell Science Plasmid Collection

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    Collection
    ...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell...

  3. Bacterial Expression Systems

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    Collection
    ...selection of plasmids and tags for protein purification. The pTD Plasmid Series contains plasmids suitable...Expansion Collection Microbiology Plasmids Collection Synthetic Biology Plasmids Collection Empty Backbones ...under the microscope. The plasmids in this collection are clone-in ready plasmids that contain fluorescent...New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for non-toxic protein labeling and...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events and molecules...interactions between proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression ...CRISPR plasmids that have been designed for their use in bacteria in our Bacterial CRISPR Plasmids page ...

  4. Corralling and Cornering the Goldilocks Antibody

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    Blog Post
    ...relatively new technology that takes advantage of DNA sequencing to turn mAb clones into expression constructs...Antibodies 101: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ...

  5. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts...specific compartment. If your proteins are nuclear- or DNA-binding proteins, you may need to sonicate your lysates...

  6. Hot Plasmids - January 2023

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    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ... 2022.   Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting... missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity ...2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP... Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C., et al. Chemically stable...novel RNA targeting system by: Susanna Stroik The Doudna lab’s recent work characterized a new tool for ...

  7. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...packaging plasmids. The cells need three different plasmids to produce AAV: 1) the RepCap plasmid which provides...going to lie, AAV transfer plasmids are kind of a pain to work with. These plasmids contain ITRs which are... AAV transfer plasmids. It’s easy to contaminate a workspace with plasmid, not that I would know about...prep. AAV samples are first DNaseI digested to remove any residual AAV plasmid that was carried over from...sample for the DNAseI digest. Use good sterile technique when handling your AAV plasmid standard and AAV...AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses adenovirus genes which help mediate...mediate AAV viral replication; and 3) the transfer plasmid which encodes a transgene of interest that’s packaged...

  8. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post
    ...We've developed a new plasmid toolkit, the Broad Institute Target Accelerator Plasmid Collection, that we...phenotypes. Broad Institute Target Accelerator Plasmid Collections To overcome this first challenge, we...developed the Broad Institute Target Accelerator Plasmid Collections, now widely available to the scientific...in our recent publications, we used the cancer plasmid collections listed above to demonstrate how gene... hope that by releasing these new and exciting plasmid collections (together with the data generated from...patients. We hope you find these new cancer variant plasmid collections useful for your studies. Many thanks... Addgene.org Fine More Kits at Addgene Browse Plasmids from the Broad Institute ...

  9. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular...need. You will also have to make sure that your plasmid has been incorporated into the host organism, usually...open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional...details and guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ...mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the CMV promoter for ...protein will allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating transiently...

  10. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

    Type
    Blog Post
    ...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...may have noticed it’s been easier to order these plasmids. This is because in 2024 certain export control...controls prescribed by the Australia Group, for these plasmids for the majority of the countries we ship to, ...lasting basis.   Table 1: Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G ... Weinberg pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope...control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster, as ...

  11. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...four different plasmids into the producer cells (two packaging plasmids, an envelope plasmid, and the lentiviral...generation systems consist of an envelope plasmid and a packaging plasmid encoding HIV proteins gag, pol, rev...generation systems require an envelope plasmid, and two packaging plasmids, one encoding gag and pol and a second...transfer plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are ... 30% of the transfer plasmid. Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal...Addgene's most popular lentiviral plasmids Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and ...collection of lentiviral plasmids created for a variety of applications including cDNA expression, shRNA-mediated...

  12. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post
    ...received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in...small collection of plasmids for coronavirus research, including a handful of plasmids that have been in...some highlights of the plasmids shared over the last year and how these plasmids have been used to accelerate...circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited from... labs also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include...These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that ...scientists through plasmid sharing and more. As you’ve read above, some of these plasmids have been in the...

  13. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...comparison of an shRNA plasmid sequence and an miRNA plasmid sequence. (A) Plasmid sequence from pLVUHshp53...between the red boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence....   Expressing shRNAs using plasmids The design of shRNA plasmids requires three important steps: ...shRNA plasmids are being designed using RNA polymerase II promoters. The design of these plasmids is meant...with Cas13a (C2c2) Plasmids 101: The Promoter Region – Let's Go! Browse All Plasmids 101 Posts Additional... occur via transfection or viral delivery of a plasmid. RNAi is an umbrella term for a number of different...Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA machinery ...

  14. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post
    ...encoded on separate plasmids and are co-transfected into cells alongside a plasmid encoding the minigenome...four viral genes in the same plasmid (as opposed to four separate plasmids), there are a few reasons arguing...separate (and thus smaller) plasmids. Finally, having four separate plasmids allows researchers to partially... 2 μg minigenome plasmid per well (in a 6 well plate format). However, since plasmid quality and transfection...Minigenome System). From a more technical standpoint, a plasmid with all four genes and necessary control elements...relatively large. Cloning the viral genes in this plasmid would be more difficult compared to if each gene...Technical tips It is important that all of these plasmids (those containing NP, VP35, VP30, L, and the minigenome...

  15. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...about a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids! Light controls...Hahn's lab at the University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 ... the same number of fluorophores. You can find plasmids containing the cobalamin binding riboswitches ...the Reuben Harris lab has deposited a reporter plasmid that allows for rapid fluorescence-based quantification...editing complexes are more efficient than the BE3 plasmid containing rat APOBEC1. Finally, they show that...

  16. Addgene: The First Twenty Years

    Type
    Blog Post
    ...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first...frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan, and Benjie...nonprofit that would facilitate the sharing of plasmids between scientists. The service would allow people... our first major repository milestone — 10,000 plasmids deposited and available — and nearly seven before...possible for scientists to both deposit and request plasmids, was a major focus of our first decade. We did...work In March 2015, Addgene reached the 500,000 plasmids distributed milestone! We were thrilled to see...also made us think of all the depositors whose plasmids we were entrusted with. So in June 2016, we launched...

  18. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...Fusions Plasmids 101: Secondary Nanobody Toolbox Resources on Addgene.org View the Antibody plasmid collection...transcribed into cDNA fragments that are efficiently subcloned into the phagemid pDX_init plasmid, using the...The Seeger lab found a way around this by using plasmids to generate sybodies, synthetic nanobodies against...nanobodies can be synthetically expressed off of plasmids in bacteria, and they can target epitopes that...form of mRNA for academic research. The template plasmids for generating your own libraries are also available...available in the Sybody Generation Toolbox plasmid kit: SB_concave, SB_loop, SB_convex. These scaffolds... subcloned into pBXNPH3 or pBXNPHM3 expression plasmids for the production of tag-free proteins for further...

  19. Neurodegeneration Research Collection

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    Collection
    ... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... species. You can also find our guide to using plasmid pooled libraries . New and Noteworthy: Use a CRISPRi...

  20. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...Resources & Plasmids Plan Your CRISPR Experiment Synthetic Biology Resources & Plasmids  ...poster were impressed to see how the numbers of plasmid genome engineering tools – both deposited and distributed...expressed gratitude for the role Addgene plays in plasmid sharing to those who were anxious to hear about...biggest names in the CRISPR-Cas9 field – Jennifer Doudna, Feng Zhang, and Keith Joung. All Addgene depositors...

  21. CRISPR References and Information

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    Collection
    ...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...HDR design; Deep sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning...PDF, 108 KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode:...

  22. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...you want to give GeoCas9 a try, as always, the plasmids are available from Addgene. Happy CRISPRing! ...Background with Addgene’s CRISPR Guide Page Find CRISPR plasmids at Addgene ...However, the recent discovery of GeoCas9 by the Doudna lab has opened up the field of thermophilic bacteria...

  23. Genetic Code Expansion

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    Collection
    ...Generally, you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and ...activity of a protein (e.g. converting a DNA binding protein to a DNA cleaving enzyme) or to regulate the ...Research Center . Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin... Farren Isaacs Browse Target Plasmids The table below highlights plasmids that contain genes with modified...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian... Plasmid Collections Genetic Code Expansion Genetic Code Expansion You may also like... GCE4All...

  24. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post
    ... [6], plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the plasmids used for...Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. Transdifferentiation...Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka Lab ... A.B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell, 1987. 51...

  25. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors Resources on Addgene.org...Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors ...over- and under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio to the gene...

  26. Zhang Lab CRISPR Page

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    Collection
    ...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described...sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which will reveal...2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice... Cas9 gRNA plasmids from the Zhang lab Genome...

  27. Tetracycline Inducible Expression

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    Collection
    ...Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids Collection Tetracycline.... ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for ...with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...search our collection for all dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...shRNA efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target. rtTA3 Ptet, miniCMV Howard...expression and find plasmids for your experiments using tet- or dox-inducible systems. Plasmid...

  29. Molecular Biology Reference

    Type
    Guide
    ...Genetic Code Recombinant DNA Working with Plasmids Plasmid Elements Types of Plasmids Molecular Cloning Common... Fluorescent Protein Plasmids , Luciferase Plasmids Viral Plasmids These plasmids are modified viral genomes...transfer called conjugation. Like chromosomal DNA, plasmid DNA is replicated upon cell division, and each...fragments of DNA from one context to another, such as from a chromosome to a plasmid or vector DNA backbone...recombinant DNA, in which many identical DNA fragments can be generated. The combination of a DNA fragment...fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule, which can be used to study... structure of a plasmid. Plasmid Element Description Origin of Replication ( ori ) DNA sequence which ...

  30. Molecular Cloning Techniques

    Type
    Guide
    ...ends in the DNA fragments. Given its prevalence, the vast majority of plasmids used for DNA cloning and...Addgene Blogs Plasmids 101: Restriction Cloning Plasmids 101: Gateway Cloning Plasmids 101: TOPO Cloning ...archaea, cleave double-stranded DNA (dsDNA) at specific sequence sites in the DNA. In restriction cloning, scientists...any piece of DNA that already has restriction sites on either side of it into any plasmid that has the...method. During Gateway, moving a piece of DNA from one plasmid into another is done via a single recombination... approach, the fragment of DNA that you would like to clone into a plasmid must already be surrounded ... ends of the DNA sequence. This fragment can then be cloned into a Gateway donor plasmid, which contains...
Showing: 451 - 480 of 509 results