Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 849 results for: HA

Showing: 601 - 640 of 849 results

  2. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...technology and rapid improvements in the field, it has recently become possible to introduce DSBs and concomitant...efficiency across different stem cell types. This has facilitated easy generation of clonal knock-in lines...or try out an alternative tag (3xFLAG, Myc, V5, or HA). Tag-specific PCR genotyping can tell if HDR completely...

  3. AAV ddPCR Titration

    Type
    Protocol
    ...sealer and press the ‘Seal’ button. After the plate has been sealed, proceed to thermocycling. Thermal Cycling...

  4. Science Guides

    Type
    Guide
    ... Science Guides Addgene has created the following guides to help you start learning...

  6. Editor's Choice, October 2016

    Type
    Blog Post
    ... of excitement at Addgene. Our new viral service has begun to pick up steam (we already have over 70 orders...

  11. Typing CRISPR Systems

    Type
    Blog Post
    ...these adaptive bacterial and archaeal immune systems has led to extreme sequence divergence that requires ...landmark publication in 2013. Since that point, it has become the most engineered Cas enzyme, with adaptations... CRISPR type that exclusively targets RNA. Cas13 has become a natural successor to traditional RNA interference...

  12. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...bridge helix. Created with BioRender.com.   Cas9 has two major lobes, called NUC (for nuclease) and REC...gray). Target DNA strand, light gray.   Once it has found a PAM site, Cas9 needs to check for complementarity...orange) and HNH (red) domains, while the REC lobe has been hidden for visibility. A RuvC loop caps the ...

  14. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...high for both plasmids, so each plasmid “thinks” it has a higher copy number than it actually does, and neither...large, low copy virulence plasmids. This strategy has been successful in displacing, or “curing” the virulence...

  16. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...instances where it does. 1) When the backbone vector has multiple BbvCI sites. If these sites have opposite...example like this. 2) When your gene of interest has a BbvCI site(s). If so, the nicking mutagenesis protocol...

  17. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...lab to split my cells later. Eating with friends has a somewhat immediate deadline - even the best of ...can be quite difficult. In my experience, planning has 3 components: A mechanism for blocking off time ...

  18. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...target is known to be absent naturally or in which it has been knocked-out/knocked-down. “No primary”, pre-... reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique...

  19. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...Cytotoxicity can also be cell type dependent: if a cell has few receptors for a specific envelope protein, it...lentiviral vector to be transported retrograde and also has higher neuronal transduction efficiency and a better...

  20. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...editing systems in bacteria. Our pCas/pTargetF system has been one of the most popular genomic editing systems...Yang lab.   In conclusion, the MUCICAT system has the following advantages: Fast and site-specific...

  21. An Introduction to Adenovirus

    Type
    Blog Post
    ... virulence (Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation...the infection to sometimes result in cell death, has led to interest in using adenoviral vectors for vaccine...

  22. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...eBook, How to Get a Faculty Position. This edition has substantial expansions on the chalk talks and Zoom...I also incorporated example documents. This part has generated a lot of positive feedback. Many of these...

  23. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...applicable in DSB-induced genome engineering, it has biological importance for the repair of breaks that...directed repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made...

  25. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...mycoplasma contamination. Mycoplasma contamination has been shown to arise from a variety of sources such...line for mycoplasma and discard it if the culture has become overly confluent or too old. Ensuring that...

  27. Addgene's Website Update

    Type
    Blog Post
    ...soon as the homepage opens. You’ll notice it now has two options: “Search Repository” and “Deposit Materials...

  35. CRISPR Library Amplification

    Type
    Protocol
    ... evenly with a sterile spreader until all liquid has been absorbed by the agar. This usually takes 1-2...

  36. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...is important to determine how much phosphorserine has actually been incorporated into the full-length protein...protein. Up to 80% p-Ser incorporation into E17TAG GFP has been achieved with the Rinehart system (6); however...

  37. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...tough job! Kamel Khalili’s lab at Temple University has demonstrated two potential strategies for CRISPR-...approach might be more successful. “Shock and kill” has the advantage of killing cells infected with HIV-...

  38. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target activity and decreased...
Showing: 601 - 640 of 849 results