We narrowed to 877 results for: HA

...development for scientists A new plasmid tool your lab has deposited If you’d like to write about a different...

...sealer and press the ‘Seal’ button. After the plate has been sealed, proceed to thermocycling. Thermal Cycling...

...sample with a good A260/280 but a low A260/230 likely has some residual guanidine thiocyanate. Phenol is a ...contamination — just be sure to check that your RNase has not expired (or been forgotten).    Improving read...

...for bimolecular fluorescence complementation BiFC has been used to directly visualize PPIs that regulate... PIPs in which the protein-interaction interface has been compromised (Kodama and Hu, 2012). Such controls...

...purify DNA, but rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the ...chaotropic salt sodium iodine (NaI) solution. NaI has two purposes in this protocol: 1) it solubilizes ...

...we should first note that quantitative PCR (qPCR) has been a powerful tool for quantifying AAV for some... 2 minutes and the denaturation time to 1 minute has been shown to increase droplet separation (Witte ...

...protocols, and look - they’re seeking someone who has, to a t, all the research skills under your belt ...their dream job; They want to know if the candidate has what it takes to thrive at the company and add value...

...? There is a difference. A mentor is someone who has gone where you hope to go, and who will invest in...Joanne Kamens, a strong advocate for career planning, has written a great blog on choosing good mentors for...

...requires. Using the (Last name, Year) format also has the benefit of exposing you to relevant researchers...just gotta do it." At the end of the day, someone has to write the article and that someone is going to...

...invention in the late 1990s, Gateway cloning technology has become very popular as a rapid and highly efficient...attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for many popular...

...coming directly from grad school. The transition has been a bit rough for her (3 weeks in and there's ...Scientist team says, “a well-run meeting where everyone has a voice and the group can come to a mutual agreement...

...native organism.” This is possibly a better idea and has been used successfully in the past (Angov et al.,...functionally analyze the protein produced to ensure that it has folded properly. You may be able to avoid getting...

...bloodstream allows for dispersion of the virus but it has to be able to cross the blood-brain barrier to reach...treatments for neurological diseases). The Gradinaru Lab has a lot going on. Here are the key takeaways: Two...

...deactivate specific genes are needed. Targeted mutation has been demonstrated in zebrafish using zinc-finger ... in all cells of the developing zebrafish embryo has been mapped using single cell RNA sequencing approaches...

...recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and rAb plasmids...plant, or mammalian cells. Each of these systems has their advantages and disadvantages. Briefly, bacterial...

...University of Miami. The beginning of this century has seen some major advances in light microscopy, particularly...unprecedented detail.  One of these advances in microscopy has been light sheet fluorescence microscopy (LSFM). ...

...transcript, you will also need to make sure your plasmid has a promoter suited to working in your host organism... but requires SP6 RNA polymerase. SP6 polymerase has a high processivity. When used for in vitro transcription...

... Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies* may even be open to dancing...

...fact, they’ve been following a set of clues that has led them to their biggest discovery yet: Addgene ...

...at Missouri University of Science and Technology, has been teaching the microbiology lab every year for...

...newest viral vector service, AAV Packaged on Request  has seen a fantastic response from the research community...

...transformation?  Great news. A subset of our collection has been prepared as part of our new cloning grade DNA...

...                     A post shared by Xi Chen (@seleena78) The lab has officially welcomed the Christmas spirit. It's just...

...multiple sites in cultured human cells. While it has not yet been demonstrated, they say the vectors are...

...technology and rapid improvements in the field, it has recently become possible to introduce DSBs and concomitant...efficiency across different stem cell types. This has facilitated easy generation of clonal knock-in lines...or try out an alternative tag (3xFLAG, Myc, V5, or HA). Tag-specific PCR genotyping can tell if HDR completely...

...as they pass our standard QC process and the MTA has been approved. The spreadsheet option is recommended...cloned into the vector backbone). If your plasmid has more than one insert, click "Save and Add Another...primers can be used to verify it. If your plasmid has multiple inserts, enter details for each insert individually...

...combining the function of both editors into one? This has been done by fusing the adenine and cytosine editing...deaminases with a Cas9 nickase (Zhang et al., 2020). It has increased CBE activity and reduced RNA off-targeting...engineering and directed evolution of the TadA domain has yielded base editors that are equally efficient at...which uses a different mechanism than base editing, has allowed scientists to replace entire sequences of...

...therapeutics to treat neurodegenerative diseases. He has earned a B.S. in microbiology and subsequently obtained...

...where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge, MA and...

...Alex is the first MD PhD we've had on the podcast, has worked extensively with CRISPR, and recently became...

... of excitement at Addgene. Our new viral service has begun to pick up steam (we already have over 70 orders...

...takes to make one plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on time...

... and microbe cookies! 🤗 🤗 🤗 We hope everyone has a safe and festive Holiday season. 🙌 🥂 🎄 🎉 😷...

...2004 to today, you can see how plasmid distribution has grown. Additionally, more and more scientists outside...

...these adaptive bacterial and archaeal immune systems has led to extreme sequence divergence that requires ...landmark publication in 2013. Since that point, it has become the most engineered Cas enzyme, with adaptations... CRISPR type that exclusively targets RNA. Cas13 has become a natural successor to traditional RNA interference...

...bridge helix. Created with BioRender.com.   Cas9 has two major lobes, called NUC (for nuclease) and REC...gray). Target DNA strand, light gray.   Once it has found a PAM site, Cas9 needs to check for complementarity...orange) and HNH (red) domains, while the REC lobe has been hidden for visibility. A RuvC loop caps the ...

...target is known to be absent naturally or in which it has been knocked-out/knocked-down. “No primary”, pre-... reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique...

...Cytotoxicity can also be cell type dependent: if a cell has few receptors for a specific envelope protein, it...lentiviral vector to be transported retrograde and also has higher neuronal transduction efficiency and a better...

...editing systems in bacteria. Our pCas/pTargetF system has been one of the most popular genomic editing systems...Yang lab.   In conclusion, the MUCICAT system has the following advantages: Fast and site-specific...

...all the learning and planning, and your MoClo kit has just arrived! MoClo kits from Addgene will most likely...further assemblies. This will help confirm each part has ligated in the right order and direction (which is...