Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 990 results for: IGH@

Showing: 601 - 640 of 990 results

  1. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...Biotin protein ligase (BPL) is responsible for this highly specific post-translational protein modification...photoreactive arylazide group. Under strong visible light, the arylazide group becomes reactive and forms ...: 20353954. 5. Kay, B., Thai, S., & Volgina, V. “High-Throughput Biotinylation of Proteins.” Methods Mol...PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled molecules on...

  2. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...donor, you can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This...provided you have some idea of where in the genome you might expect off target editing to occur. Luckily, in ...you to Sanger sequence a manageable number of the higher probability locations. If you’re worried about ...Bombieri N, Giugno R, Pinello L. CRISPRitz: rapid, high-throughput and variant-aware in silico off-target...

  3. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...overview of plant genome engineering using CRISPR, highlight the specific modifications to CRISPR machinery...Lab.  As with all plasmids in the repository, we highly recommend reading the associated publications or...10.1104/pp.15.00636 Ma X, Zhang X, Liu H, Li Z (2020) Highly efficient DNA-free plant genome editing using virally...

  4. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...to make them serve you well. The happy byproduct might just be a career opportunity. The focus of this...content. Create a Google.slides or Powerpoint file right now to capture interesting key results, striking...need to define a term, but are worried that this might sound condescending, consider explaining the term...Additional Resources Joanne's "How to Choose the Right Next Lab or Workplace" On YouTube Joanne's 10 Things...

  5. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... can find his original blog post below! --Ed.  Eight years ago, I decided to write a "how to" manual ... and mentoring to several people that found my insights helpful. All went on to get faculty positions ...potential colleagues study. Think about how you might collaborate or, at the absolute minimum, can appreciate...free!) contains strategies, hints, anecdotes, and insights. After I wrote the first version of my manual,...

  6. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... from a default OFF to an ON state, and how you might use these in your research. Today, we’ll look at...Take a look at all of the options and decide what might work best for your experiments! This post was originally...References and Resources References Gossen M, Bujard H. Tight control of gene expression in mammalian cells by...PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection of VP16, the trans-activator...

  7. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ... site.  T-RL= transposon right-left orientation. T-LR= transposons left-right orientation. Image from ...2009). hySB100x improves on SB100X by having 30% higher transposition activity (Voigt et al., 2016). Sleeping...sequence into the zygote’s genome, a process that’s highly unpredictable. Transposons, however, efficiently...

  8. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...backwards. To publish a submitted manuscript in a high-profile journal typically takes 6-12 months depending...technology may not exist to answer the question or it might be extremely difficult for you to get the needed...for a summer undergrad, rotating grad student or high school student. Learn to teach how to design, perform...improves your perspective like intense exercise. I highly recommend running or biking or something that demands...

  9. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...activated, the protein emits a brief yet bright burst of light, visible to the naked eye, at regular intervals...forever change the fly lab experience. The Frequency Light Interval System, or FLIS, incorporates a bioluminescence...

  10. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...Addgene we couldn’t be more excited! As a small, tight-knit organization we thrive on 3 things: fun, teamwork...trophy among 6 people, but you’ve earned bragging rights for the next 12 months. Your team’s creativity,... favorite lunch... a BonMe noodle meal, crammed right into the box. Which is your favorite team costume...

  11. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...service pAAV_hSyn1_nLightG AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors...of synapses distant to the site of injection with high temporal precision.   Table 1: New ready-to-use...

  12. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    ...targeted destruction.  Antibodies are composed of two light chains and two heavy chains. The ends of the branches...branches contain the antigen binding sites and are highly variable, allowing exquisite specificity. This ...rabbit antibodies that exhibit increased avidity to high profile antigens; and 4) generating mAbs against...

  13. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ...little screening for DNA markers like the presence of high risk (HR) Human Papillomavirus (HPV). As Gladys ...individuals who are infected with HR HPV and therefore at high risk for cervical cancer development and also better...disease severity. Individuals who are shown to be at high risk by these screens can then undergo more routine...

  14. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    ... a few easy and fast things you can start doing right away to reduce your consumption in the lab: Turn...your oven on all day to save time making dinner at night, so why leave your lab equipment on? This applies...fact, some scientists speculate the -80 °C setting might have been a marketing campaign! MIT Green Labs has...

  15. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...accelerate research and discovery by improving access to high-quality scientific materials and information. Our... repository of valuable research materials that might otherwise be lost amid lab closures or project terminations...Providing essential services: We continue to offer high-quality DNA sequencing, viral vector preparations...

  16. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...only two options, but very little data on which might work in your application. It can be quite difficult...materials and methods section. This section will vary slightly depending on the application — flow cytometry,...Antibodies 101: Validation Antibodies 101: Selecting the Right Antibody...

  17. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....recognizes a simple dinucleotide PAM (N4CC), lending to high target site density (average every 8 bp). Nme2Cas9...

  18. Addgene's Website Update

    Type
    Blog Post
    ...look and feel of addgene.org. Some you’ll notice right away, while others may be less obvious — but every...you can use the links under the search box to go straight to your materials of interest!   Figure 1: The...bar New menu Most common activities can be done straight from the new search bar, but the menu just above...

  19. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ... also selected for other key properties, notably high solubility, limited affinity for other proteins,...interactions, particularly in single-molecule or high-throughput applications. Perhaps IT5 and IT6 will...Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian...

  21. Science Communication for Everyone

    Type
    Blog Post
    ...physicists, chemists, and mathematicians. We hope to highlight every science field as we expand and encourage... This would help you direct your content to the right audience. Avoid using scientific jargons. Someone...

  22. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....identified 67 genera of Proteobacteria that this toolkit might be used in. Read the paper in ACS Synthetic Biology...

  23. Editor's Choice, September 2016

    Type
    Blog Post
    ...pleasure to announce that we once again reached new heights on the Addgene blog: we surpassed 60,000 views ...researcher at Sanofi Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia ossificans progressiva...

  24. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...science accessible in one way or another to those who might be in a position to build on that work. That’s how...to consider where the open access (OA) movement might be going is to consider some of the places it’s ...providers and participation from many well-respected and high-profile universities. We particularly recommend ...

  25. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ...functionally validating the thousands of mutations that might play a role in the progression of cancer are often...mask weaker ones. Thus, the need for a faster and higher-throughput in vitro approach. Using data from The..., they generated lentiviral constructs using the High-Throughput Mutagenesis and Molecular Barcoding (...

  26. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...directory is crucial because it's hard to find the right phage for the job. Phages are viruses that can infect... have someone that really needs it, but then it might to be too late,” Sacher says. As the labs in the...sample is limited, only the labs that have the highest potential to possess the correct phages will test...

  27. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...excitation at 488 nm, photoconverted with 405 nm light, and then captured in the red state after excitation...photoconverted, so this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have...Murphy CS, Hazelwood KL, Davidson MW, Looger LL. A bright and photostable photoconvertible fluorescent protein...

  28. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...within the scientific community The team behind Crowdfight COVID-19 strives to match up scientists across...with different technical knowledge that could help fight COVID-19. They’ve also collated a list of useful...  "We realized that there is now a huge pool of highly skilled scientists, willing to volunteer their ...

  29. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....that controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters...

  31. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....and human cells. Using the structure of CasX at eight different states of assembly from cryo-electron ...

  32. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...research and expanded choice when it comes to ordering high-quality materials from the Addgene repository. Read... be aware that this ordering process will look slightly different from our standard process. We’ll walk... and flexibility; while ensuring we can maintain high standards for both our depositors and requestors...

  33. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ...efficient capture. We wanted to be able to build a highly multivalent assembly connected by covalent bonds...St. Andrews discovered the domain. It is small, highly stable and has this amazing chemistry locking the... the variation in cancer, not just the CTCs with high marker expression level. Labs have been trying different...

  34. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ... has been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive 1,350... Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many more! Baird...know how all the parts go together, we can fix it. Right now, we have no idea." Now, it will be that much...

  35. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    ...Cas9, then with the sgRNAs, and can result in a higher titer due to the smaller size of the plasmids. ...of selection. Shalem and Sanjana say they kept a high representation of library within cells and aimed... construct, but in some biological systems one “might need larger numbers.” Further details and protocols...

  36. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ... available, and have deep tissue penetration and high spatial resolution. By piecing together genes from...are sequentially exposed to acoustic pulses with high enough amplitudes to first collapse arg2 and then...development of ARGs and have already developed a high-throughput screening method for serial acoustic ...

  37. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ... these results, it’s clear that editing is much higher in the PAM-out configuration. In addition, D10A...D10A editing efficiency is consistently higher than that of H840A, especially with smaller nick distances...cuts close to your insertion site, you can’t obtain high HDR efficiency. Nickases create a staggered cut ...

  38. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ... Hill. Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method... purified for subsequent library preparation and high-throughput sequencing. Improvements to the CUT&RUN...challenging to collect large numbers of cells or when a high degree of signal to noise is desired. It also provides...

  39. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ... in signaling processes including smell, taste, sight, inflammation, and neurotransmission. But with approximately...activated downstream of GPCRs that are not involved in sight (Olsen RHJ et al., 2020). A transducer complex consists...Gγ-GFP2 will be in close association, resulting in a high level of GFP2 emission. Following activation in ...

  40. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...protocol, you might have noticed that there are many options to elute your DNA prep. You might see protocols...denature, break, and even change the DNA sequence. High pH favors the breakage of the hydrogen bonds, destabilizing...
Showing: 601 - 640 of 990 results